NLRC3 attenuates antiviral immunity and activates inflammasome responses in primary grouper brain cells following nervous necrosis virus infection

Krishnan, Rahul; Rajendran, Rahul; Jang, Yo-Seb; Kim, Jong‐Oh; Yoon, Su-Young; Oh, Myung‐Joo

doi:10.1016/j.fsi.2022.06.026

Cited by 10 publications

(2 citation statements)

References 53 publications

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“…Notably, one NLR family member, NLRC3, was found to be significantly downregulated at 10 °C but upregulated at 20 °C post HIRRV. In teleost, the NLRC3 of grouper acts as a host factor that negatively regulated the antiviral immune response to facilitate nervous necrosis virus replication [ 43 ]; however, zebrafish NLRC3-like protein could interact with SVCV, and has positive regulatory function during SVCV infection [ 44 ]. So, the regulatory role of flounder NLRC3 in antiviral immunity remains to be further elucidated.…”

Section: Discussionmentioning

confidence: 99%

Proteomic and Phosphoproteomic Analysis Reveals Differential Immune Response to Hirame Novirhabdovirus (HIRRV) Infection in the Flounder (Paralichthys olivaceus) under Different Temperature

Tang,

Zhang,

Xing

et al. 2023

Biology

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Hirame novirhabdovirus (HIRRV) is one of most serious viral pathogens causing significant economic losses to the flounder (Paralichthys olivaceus)-farming industry. Previous studies have shown that the outbreak of HIRRV is highly temperature-dependent, and revealed the viral replication was significantly affected by the antiviral response of flounders under different temperatures. In the present study, the proteome and phosphoproteome was used to analyze the different antiviral responses in the HIRRV-infected flounder under 10 °C and 20 °C. Post viral infection, 472 differentially expressed proteins (DEPs) were identified in the spleen of flounder under 10 °C, which related to NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, RNA transport and so on. Under 20 °C, 652 DEPs were identified and involved in focal adhesion, regulation of actin cytoskeleton, phagosome, NOD-like receptor signaling pathway and RIG-I-like receptor signaling pathway. Phosphoproteome analysis showed that 675 differentially expressed phosphoproteins (DEPPs) were identified in the viral infected spleen under 10 °C and significantly enriched in Spliceosome, signaling pathway, necroptosis and RNA transport. Under 20 °C, 1304 DEPPs were identified and significantly enriched to Proteasome, VEGF signaling pathway, apoptosis, Spliceosome, mTOR signaling pathway, mRNA surveillance pathway, and RNA transport. To be noted, the proteins and phosphoproteins involved in interferon production and signaling showed significant upregulations in the viral infected flounder under 20 °C compared with that under 10 °C. Furthermore, the temporal expression profiles of eight selected antiviral-related mRNA including IRF3, IRF7, IKKβ, TBK1, IFIT1, IFI44, MX1 and ISG15 were detected by qRT-PCR, which showed a significantly stronger response at early infection under 20 °C. These results provided fundamental resources for subsequent in-depth research on the HIRRV infection mechanism and the antiviral immunity of flounder, and also gives evidences for the high mortality of HIRRV-infected flounder under low temperature.

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Section: Discussionmentioning

confidence: 99%

Proteomic and Phosphoproteomic Analysis Reveals Differential Immune Response to Hirame Novirhabdovirus (HIRRV) Infection in the Flounder (Paralichthys olivaceus) under Different Temperature

Tang,

Zhang,

Xing

et al. 2023

Biology

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“…The NLR family includes three main sub-families: the Nods (nucleotide-binding oligomerization domain containing 1 (Nod1), nucleotide-binding oligomerization domain containing 2 (Nod2) [ 9 ], nucleotide-binding oligomerization domain containing 3(Nod3)/nucleotide-oligomerization domain-like receptor subfamily C3 (Nlrc3) [ 10 ], nucleotide-binding oligomerization domain containing 4 (Nod4), nucleotide-binding oligomerization domain containing 5 (Nod5)/nucleotide-binding domain and leucine-rich repeat-containing protein X1 (Nlrx1) [ 11 ], and Class II major histocompatibility complex transactivator (CIITA), the Nlrps (Nlrp1–14, also known as NACHT-LRR-PYD-containing proteins (Nalps) [ 11 ]), and the Ipaf subfamily (nucleotide-oligomerization domain-like receptor subfamily C4 (Nlrc4) [ 12 ] and neuronal apoptosis inhibitory protein (Naip) [ 13 ]). The inflammasome generally consists of NLRs, adaptor apoptosis-associated speck-like protein(ASC), and caspase-1, which regulate the maturation and secretion of the proinflammatory cytokines IL-1β and IL-18, and initiate inflammatory processes [ 14 ].…”

Section: Nod-like Receptorsmentioning

confidence: 99%

How Gut Microbiota Are Shaped by Pattern Recognition Receptors in Colitis and Colorectal Cancer

Qing

Xie

et al. 2022

Cancers

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Disorders of gut microbiota have been closely linked to the occurrence of various intestinal diseases including colitis and colorectal cancer (CRC). Specifically, the production of beneficial bacteria and intestinal metabolites may slow the development of some intestinal diseases. Recently, it has been proposed that pattern recognition receptors (PRRs) not only recognize pathogens and initiate inflammatory signal transduction to induce immune responses but also influence the composition of intestinal microorganisms. However, the mechanisms through which PRRs regulate gut microbiota in the setting of colitis and CRC have rarely been systematically reviewed. Therefore, in this paper, we summarize recent advances in our understanding of how PRRs shape gut microbiota and how this influences the development of colitis and CRC.

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In vitro analysis of the expression of inflammasome, antiviral, and immune genes in an Oreochromis niloticus liver cell line following stimulation with bacterial ligands and infection with tilapia lake virus

Dinakaran,

Prasad,

Bedekar

et al. 2024

Arch Virol

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NLRC3 attenuates antiviral immunity and activates inflammasome responses in primary grouper brain cells following nervous necrosis virus infection

Cited by 10 publications

References 53 publications

Proteomic and Phosphoproteomic Analysis Reveals Differential Immune Response to Hirame Novirhabdovirus (HIRRV) Infection in the Flounder (Paralichthys olivaceus) under Different Temperature

Proteomic and Phosphoproteomic Analysis Reveals Differential Immune Response to Hirame Novirhabdovirus (HIRRV) Infection in the Flounder (Paralichthys olivaceus) under Different Temperature

How Gut Microbiota Are Shaped by Pattern Recognition Receptors in Colitis and Colorectal Cancer

In vitro analysis of the expression of inflammasome, antiviral, and immune genes in an Oreochromis niloticus liver cell line following stimulation with bacterial ligands and infection with tilapia lake virus

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