Nitrogen removal and mechanism of an extremely high-ammonia tolerant heterotrophic nitrification-aerobic denitrification bacterium Alcaligenes faecalis TF-1

Zhang, Qian; Zhu, Yunan; Yuan, Chunbo; Zhang, Chu; Cui, Minglei; Zhao, Tiantao

doi:10.1016/j.biortech.2022.127643

Cited by 16 publications

(3 citation statements)

References 34 publications

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“…Recent studies have shown that NO 2 − -N can be toxic to microorganisms when its concentration exceeds 30 mg/L [42]. However, some HN-AD strains, such as Alcaligenes faecalis WY-01 and TF-1 strains, lacked the capacity to perform independent aerobic denitrification [4,43] and therefore could not effectively remove nitrite nitrogen. Strain L2 was able to remove NH 4 + -N, NO 3 − -N, and NO 2 − -N and showed good HN-AD function, showing great potential for the biological treatment of wastewater.…”

Section: Aerobic Denitrificationmentioning

confidence: 99%

“…Different types of N sources could affect the nitrification performance of heterotrophic nitrifying bacteria. Meanwhile, the accumulation of NO 3 − -N and NO 2 − -N might inhibit the expression of nitrite reductase and nitrate reductase during nitrification [43]. Therefore, experiments were conducted using a mixture of different N sources in a ratio of 1:1 to investigate the denitrification characteristics of strain L2 and to determine its denitrification pathway.…”

Section: Effect Of Mixed Nitrogen Sources On the Nitrification Functi...mentioning

confidence: 99%

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Heterotrophic Nitrification–Aerobic Denitrification by Bacillus sp. L2: Mechanism of Denitrification and Strain Immobilization

Li,

He,

Wang

et al. 2024

Water

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The biological denitrification of low-C/N wastewater is a great challenge in treatment plants due to the lack of microorganisms with heterotrophic nitrification–aerobic denitrification (HN-AD) abilities. In this study, Bacillus sp. L2 was isolated from aeration tank water samples using a nitrification medium and screened for its ability to perform HN-AD in low-C/N wastewater. The strain showed a maximum NH4+-N removal rate of 98.37% under low-C/N conditions. In the presence of a mixed N source, strain L2 was capable of completely removing NH4+-N within 24 h. Furthermore, optimal nitrogen removal conditions for strain L2 were found to be C/N = 9, pH = 9, and sodium acetate as the C source. Under optimal conditions, the strain was able to maintain a high NH4+-N removal rate under 0–3% salinity and an NH4+-N concentration of 200 mg/L or less. The denitrification pathways of strain L2 were NH4+→NH2OH→NO2−(↔NO3−)→NO→N2O→N2 and NH4+→NH2OH→NO→N2O→N2. Furthermore, semi-continuous wastewater treatment was conducted using immobilized technology, which resulted in more than 82% NH4+-N removal after three cycles of reuse. This study demonstrates the great potential of Bacillus sp. L2 in wastewater treatment applications.

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Section: Aerobic Denitrificationmentioning

confidence: 99%

Section: Effect Of Mixed Nitrogen Sources On the Nitrification Functi...mentioning

confidence: 99%

Heterotrophic Nitrification–Aerobic Denitrification by Bacillus sp. L2: Mechanism of Denitrification and Strain Immobilization

Li,

He,

Wang

et al. 2024

Water

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“…Genetic analysis further revealed that this functional gene cluster was phylogenetically conserved in genus Alcaligenes (Hou et al 2022 ). Several bacterial strains belonging to the genus Alcaligenes had been isolated from environmental samples, and their nitrogen performance had been characterized using synthetic (Chen et al 2021 ; Joo et al 2005b ; Zhang et al 2022 ) or real wastewater (e.g., piggery wastewater) (Joo et al 2006 ). However, prior to the identification of Dirammox, these isolates may have been wrongly regarded as bacteria involved in the HNAD process.…”

Section: Introductionmentioning

confidence: 99%

Dirammox-dominated microbial community for biological nitrogen removal from wastewater

Hu,

Wang,

Wang

et al. 2024

Appl Microbiol Biotechnol

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Direct ammonia oxidation (Dirammox) might be of great significance to advance the innovation of biological nitrogen removal process in wastewater treatment systems. However, it remains unknown whether Dirammox bacteria can be selectively enriched in activated sludge. In this study, a lab-scale bioreactor was established and operated for 2 months to treat synthetic wastewater with hydroxylamine as a selection pressure. Three Dirammox strains (Alcaligenes aquatilis SDU_AA1, Alcaligenes aquatilis SDU_AA2, and Alcaligenes sp. SDU_A2) were isolated from the activated sludge, and their capability to perform Dirammox process was confirmed. Although these three Dirammox bacteria were undetectable in the seed sludge (0%), their relative abundances rapidly increased after a month of operation, reaching 12.65%, 0.69%, and 0.69% for SDU_A2, SDU_AA1, and SDU_AA2, respectively. Among them, the most dominant Dirammox (SDU_A2) exhibited higher nitrogen removal rate (32.35%) than the other two strains (13.57% of SDU_AA1 and 14.52% of SDU_AA2). Comparative genomic analysis demonstrated that the most dominant Dirammox bacterium (SDU_A2) possesses fewer complete metabolic modules compared to the other two less abundant Alcaligenes strains. Our findings expanded the understanding of the application of Dirammox bacteria as key functional microorganisms in a novel biological nitrogen and carbon removal process if they could be well stabilized. Key points • Dirammox-dominated microbial community was enriched in activated sludge bioreactor. • The addition of hydroxylamine played a role in Dirammox enrichment. • Three Dirammox bacterial strains, including one novel species, were isolated. Graphical abstract

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Denitrification characteristics and potential application of a novel aerobic denitrifying bacterium, Pseudomonas plecoglossicida, isolated from the Exopalaemon carinicauda pond

Shen,

Zhou,

Qian

et al. 2024

Aquacult Int

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Nitrogen removal and mechanism of an extremely high-ammonia tolerant heterotrophic nitrification-aerobic denitrification bacterium Alcaligenes faecalis TF-1

Cited by 16 publications

References 34 publications

Heterotrophic Nitrification–Aerobic Denitrification by Bacillus sp. L2: Mechanism of Denitrification and Strain Immobilization

Heterotrophic Nitrification–Aerobic Denitrification by Bacillus sp. L2: Mechanism of Denitrification and Strain Immobilization

Dirammox-dominated microbial community for biological nitrogen removal from wastewater

Denitrification characteristics and potential application of a novel aerobic denitrifying bacterium, Pseudomonas plecoglossicida, isolated from the Exopalaemon carinicauda pond

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