A number of representative species of obligate methane-oxidizing bacteria were surveyed for their ability to fix N2 by growth experiments and the acetylene reduction test. Although all strains exhibited growth on nitrogen-free plates, only type I1 organisms and the type X methanotroph Methylococcus capsulatus (Bath) grew well in nitrogen-free liquid medium and were capable of active acetylene reduction. N,-fixation in type I1 methanotrophs was less sensitive to 0, than in the type X methanotroph Methylococcus…
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