Nitrogen-deregulated mutants of Phanerochaete chrysosporium ?a lignin-degrading basidiomycete

Boominathan, K.; Dass, Sairia; Randall, Thomas A.; Reddy, C. A.

doi:10.1007/bf00245259

Cited by 33 publications

(25 citation statements)

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“…However, this sequence was not found in the published sequences of LIP genes. Our inability to find CRE-or AP-2-like sequences in the 5' regions of LIP genes is not entirely surprising since our previous data with the lip mutant (10) indicated that the product of a single genetic regulatory element controls the expression of the LIP gene family. Hence, it is possible that the CRE or AP-2 sequences, if involved in the regulation of expression of LIP gene family, might be located on a regulatory control locus defined by the lip mutation.…”

Section: Discussionmentioning

confidence: 67%

“…Northern blot analyses were performed to determine whether cAMP inhibitors affect LIP and MNP production at the level of transcription. First, we used atropine as the cAMP inhibitor of choice because it is known to directly inhibit cAMP synthesis by inhibiting adenylate C inhibitor-treated and untreated cultures were fractionated by FPLC as described (10,11). The heme protein peaks were designated as in ref.…”

Section: Resultsmentioning

confidence: 99%

“…Cultures grown in low nitrogen medium (9) were harvested on the sixth day of incubation, and the mycelia were separated by aseptically filtering the cultures through four layers of cheese cloth. The filtrate was concentrated 50-fold by using an Amicon PM10 membrane (10-kDa cutoff; Amicon) and analyzed by FPLC as described (10,11).…”

Section: Methodsmentioning

confidence: 99%

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cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium.

Boominathan

Reddy

1992

Proc. Natl. Acad. Sci. U.S.A.

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Lignin peroxidases (LIPs) and manganesedependent peroxidases (MNPs) are major components of the -ignin-degrading enzyme system of Phanerochaete chrysosporium and typically appear during secondary metabolism. The involvement of cAMP in the regulation of production of LIPs and MNPs was investigated in this study. Production of LIPs and MNPs was preceded by a sharp rise in intracellular cAMP concentration. Addition of atropine, theophylline, or histamine to cultures resulted in a drop in intracellular cAMP concentration and a concomitant inhibition of production of LIPs only or of both LIPs and MNPs, depending on the concentration of the inhibitor added. These results were independently confirmed by fast protein liquid chromatographic proffles of the LIPs and MNPs in the extracellular fluid of the inhibitor-treated and untreated control cultures. LIP production was generally more sensitive to the inhibitors than MNP production. Northern blot analyses showed that the inhibitors affect the production of LIPs and MNPs at the level of transcription. Furthermore, LIP and MNP gene expression appears to be differentially regulated depending on the intracellular concentration of cAMP. These results show that cAMP plays a key role in the regulation of production of LIPs and MNPs in P. chrysosporium.The white-rot basidiomycete Phanerochaete chrysosporium has been the focus of intense worldwide research from the standpoint of lignin biodegradation and bioremediation of environmental pollutants (for review, see refs.

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Section: Discussionmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

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cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium.

Boominathan

Reddy

1992

Proc. Natl. Acad. Sci. U.S.A.

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“…In parallel, a decline in ligninolytic and cellulolytic enzyme activities has been observed in the forest floor [19]. Under laboratory conditions, high inorganic nitrogen concentrations can repress transcription of lignocellulolytic genes [53], [54]. This raises the question: might anthropogenic nitrogen deposition elicit a similar effect?…”

Section: Resultsmentioning

confidence: 99%

Fungi Unearthed: Transcripts Encoding Lignocellulolytic and Chitinolytic Enzymes in Forest Soil

Kellner

Vandenbol

2010

PLoS ONE

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BackgroundFungi are the main organisms responsible for the degradation of biopolymers such as lignin, cellulose, hemicellulose, and chitin in forest ecosystems. Soil surveys largely target fungal diversity, paying less attention to fungal activity.Methodology/Principal FindingsHere we have focused on the organic horizon of a hardwood forest dominated by sugar maple that spreads widely across Eastern North America. The sampling site included three plots receiving normal atmospheric nitrogen deposition and three that received an extra 3 g nitrogen m−2 y−1 in form of sodium nitrate pellets since 1994, which led to increased accumulation of organic matter in the soil. Our aim was to assess, in samples taken from all six plots, transcript-level expression of fungal genes encoding lignocellulolytic and chitinolytic enzymes. For this we collected RNA from the forest soil, reverse-transcribed it, and amplified cDNAs of interest, using both published primer pairs as well as 23 newly developed ones. We thus detected transcript-level expression of 234 genes putatively encoding 26 different groups of fungal enzymes, notably major ligninolytic and diverse aromatic-oxidizing enzymes, various cellulose- and hemicellulose-degrading glycoside hydrolases and carbohydrate esterases, enzymes involved in chitin breakdown, N-acetylglucosamine metabolism, and cell wall degradation. Among the genes identified, 125 are homologous to known ascomycete genes and 105 to basidiomycete genes. Transcripts corresponding to all 26 enzyme groups were detected in both control and nitrogen-supplemented plots.Conclusions/SignificanceMany of these enzyme groups are known to be important in soil turnover processes, but the contribution of some is probably underestimated. Our data highlight the importance of ascomycetes, as well as basidiomycetes, in important biogeochemical cycles. In the nitrogen-supplemented plots, we have detected no transcript-level gap likely to explain the observed increased carbon storage, which is more likely due to community changes and perhaps transcriptional and/or post-transcriptional down-regulation of relevant genes.

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“…In a previous study, we developed a potent cellulase mutant of Pleurotus florida, designated as PfCM4, induced by gamma radiation at a dose of LD 99 (0.51 kGy) (Sathesh-Prabu and Lee, 2012) that showed 17.24% more cellulolytic activity than wild type (p < 0.05). Boominathan et al, (1990) observed that gamma-ray radiation can change the genetic diversity of filamentous fungi and induce positive mutants. The genetic similarity of mycelia and basidiospores was altered according to the dose of gamma radiation (Lee and Chang, 1999).…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

Sathesh‐Prabu

Lee

2016

Polish Journal of Microbiology

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A b s t r a c tWe report the genetic similarity changes between a mutant mushroom (Pleurotus florida, designated as PfCM4) having increased cellulolytic activity developed through radiation mutagenesis and its wild type by amplified fragment length polymorphism (AFLP). On average, 23 AFLP fragments were amplified per primer combination, and a total of 286 polymorphic fragments (78.57% polymorphism) with maximal fragment length of 1365 base pairs (bp) were obtained. The genetic similarity between wild type and PfCM4 was found to be 22.30%. In addition, mycelial and secreted protein profiling by 2D-PAGE showed at least three and five different protein spots in the range of 25 kD to 100 kD, respectively, in PfCM4. It seems that the variation in genetic similarity and different expression of both mycelial and secreted proteins in PfCM4 in comparison to the wild type could likely be correlated with its increased cellulolytic activity effected by the irradiation.

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Nitrogen-deregulated mutants of Phanerochaete chrysosporium ?a lignin-degrading basidiomycete

Cited by 33 publications

References 42 publications

cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium.

cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium.

Fungi Unearthed: Transcripts Encoding Lignocellulolytic and Chitinolytic Enzymes in Forest Soil

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

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