Nitrogen-containing phorbol derivatives of Sapium indicum

Tigliane-Type Diterpene Esters fromSynadenium grantii1,5

“…As with 06, the ct-position of 5-OH in 04 was independently proved by NOB experiments. The 12,13-diesters and 12-monoesters of 2a were previously described in an investigation of the constituents of Sapiurn indicurn (19,20) as judged from the chemical shifts of key protons in the 1H-NMR spectra. By comparison 03 and 04 turned out to be 12,13-diesters.…”

Four new phorbol diesters from Croton tiglium and their cytotoxic activities

“…2). These signals suggested that compound 1 possessed the 4-deoxy-4a-phorbol or 4-deoxy-4b-phorbol backbone (Taylor et al, 1981;Chumkaew et al, 2003 (Zhang et al, 2013). The chemical shift values of C-1, C-2 and C-10 at d C 159.7, 136.4 and 54.1 also verified the b configuration of H-4 in compound 1 (Chumkaew et al, 2003).…”

“…For type I skeleton, the chemical shift of H-1is usually in the range of 7.58-7.62 ppm, while the H 2 -5 often appear in the region of 2.5 and 2.6 ppm, respectively (Zhang et al, 2013). For type II and III backbones, the chemical shifts of H-1, H-4 and H-5 are all obviously different (Chumkaew et al, 2003;Taylor et al, 1981). The presence of two signals at around 7.04 (H-1) and 2.7 (H-4) ppm suggests the type II compounds, while the appearance of two peaks at around 7.55 (H-1) and 2.5 (H-4) ppm indicates the type III ones.…”

The stimulation of phosphorylation of intracellular proteins in GH3 rat pituitary tumour cells by phorbol esters of distinct biological activity

“…Penicillin/streptomycin solution, donor horse serum, fetal calf serum and Ham's F10 culture medium were obtained from Gibco. The phorbol esters SAP A, c~-SAP Ac and DOPPA were isolated as reported [17][18][19]. TRH and GH3 cells were kindly donated by A.H. Drummond.…”