Nitric oxide produced by ultraviolet-irradiated keratinocytes stimulates melanogenesis.

Romero-Graillet, Christine; Aberdam, Édith; Clement, M.G.; Ortonne, Jean‐Paul; Ballotti, Robert

doi:10.1172/jci119206

Cited by 270 publications

(215 citation statements)

References 52 publications

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“…Active PKC then phosphorylates tyrosinase, leading to its activation (Park and Gilchrest, 1999). Recently, there are reports that cGMP signal transduction pathway stimulates melanogenesis (Romero-Graillet et al, 1996;Romero-Graillet et al, 1997). The signal transduction pathway in GR-mediated stimulation of melanogenesis requires further study.…”

Section: Discussionmentioning

confidence: 98%

Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells

Jung

Yang²,

Song³

et al. 2001

Exp Mol Med

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Glycyrrhizin (GR), triterpenoid saponin composed of one glycyrrhetinic acid (GA) and two glucuronic acids, is a main constituent of the hydrophilic fraction of licorice (Glycyrrhiza glabra) extracts and is known to have a wide range of pharmacological actions. In this study, we investigated the mechanism of GR effect on melanogenesis in B16 murine melanoma cells. The cellular levels of tyrosinase mRNA, protein, enzyme activities and melanin contents were increased by GR in a dose dependent manner. Expression of tyrosinase-related protein-2 (TRP-2) mRNA was also increased by GR, however, no significant change was observed on TRP-1. No cytotoxicity was observed at the effective concentration range of GR. GA showed no effect on melanogenesis at the equivalent nontoxic concentrations, indicating that glycoside structure is important in the stimulatory effect of GR on melanogenesis. These results indicate that GR-induced stimulation of melanogenesis is likely to occur through the transcriptional activation.

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Section: Discussionmentioning

confidence: 98%

Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells

Jung

Yang²,

Song³

et al. 2001

Exp Mol Med

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“…Among keratinocyte-secreted factors, which induce melanocyte activation, we find prostaglandins PGE2 (32), a-MSH and ACTH (33), endothelin-1 (34,35) and NO (20). a-MSH, ACTH and PGE2 activate the cAMP pathway in melanocytes, while NO activates the cGMP-dependent signaling events.…”

Section: Indirect Effects Of Uvmentioning

confidence: 99%

Cyclic AMP a Key Messenger in the Regulation of Skin Pigmentation

Buscà

Ballotti

2000

Pigment Cell Research

727

640

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In humans, stimulation of skin pigmentation over the basal constitutive level, which we commonly call tanning, is physiologically stimulated by ultraviolet (UV) radiation of the solar light. UV-induced skin darkening involves an increase in the melanocyte number as well as a stimulation of melanin neosynthesis and melanocyte dendricity, a crucial morphological feature required for melanin transfer to keratinocytes. These events, corresponding to the final steps of melanocyte differentiation, play a central role in the tanning response and are subjected to an accurate research which aims to understand the precise mechanisms governing their regulation. In the present review, we will mainly focus our attention on the molecular processes involved in the regulation of melanogenesis.

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“…by various factors, including UV light, oxidation and keratinocytes (31,32). The association between melanocytes and keratinocytes is essential during the pathogenesis of vitiligo; however, few studies have focused on the effects of melanocytes on healthy keratinocytes.…”

Section: Discussionmentioning

confidence: 99%

“…During vitiligo treatment, keratinocytes adjacent to melanocytes contribute to UV-induced skin pigmentation (24,25); however, the precise functional effects of phototherapy and melanocytes on keratinocytes remain unknown. Therefore, the aim of the present study was to investigate the effects induced by clinical doses of BB-UVA, NB-UVB and melanocytes on human keratinocytes in vitro.…”

Section: Introductionmentioning

confidence: 99%

The effects of phototherapy and melanocytes on keratinocytes

Tang

et al. 2018

Exp Ther Med

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Abstract. Phototherapy is widely used in the treatment of vitiligo. Previous studies have focused on the effects of ultraviolet (UV) radiation on melanocytes; however, the biological effects of phototherapy and melanocytes on keratinocytes remain to be elucidated. To investigate and assess the effects of clinically doses of broad band (BB)-UVA, narrow band (NB)-UVB and melanocytes on human keratinocytes in vitro, clinical doses of BB-UVA or NB-UVB radiation and human melanoma cell A375 co-culture were performed as stress divisors to HaCaT cells. Cell proliferation, expression of protease-activated receptor-2 (PAR-2) and nuclear factor E2-related factor 2 mRNA, lipid peroxidation and intracellular antioxidant level of keratinocytes were analyzed. It was demonstrated that UV radiation inhibited the proliferation of cells apart from following exposure to low dose (1 J/cm 2 ) UVA. Medium dose (5 J/cm 2 ) UVA radiation had no adverse effects on lipid peroxidation and increased antioxidant levels in HaCaT cells. Medium (200 mJ/cm 2 ) and high (400 mJ/cm 2 ) doses of UVB radiation induced cellular damage due to increased lipid peroxidation as indicated by levels of malondialdehyde. Furthermore, A375 co-culture treatment induced a similar effect on the lipid peroxidation of HaCaT as with low dose UVB radiation. Therefore, the results of the present study determined that clinical doses of BB-UVA and NB-UVB radiation had varying effects on proliferation and related protein levels in HaCaT cells. Co-culture with A375 had similar effects as those of low dose UVA and UVB radiation, in which the PAR-2 expression was significantly upregulated.

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Nitric oxide produced by ultraviolet-irradiated keratinocytes stimulates melanogenesis.

Cited by 270 publications

References 52 publications

Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells

Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells

Cyclic AMP a Key Messenger in the Regulation of Skin Pigmentation

The effects of phototherapy and melanocytes on keratinocytes

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