Nitric Oxide Levels Total Antioxidant and Oxidant Capacity in Cattle with Foot and Mouth Disease

Abstract

The aim of this study was to investigate total antioxidant (TAC), oxidant capacity (TOC) and nitric oxide levels (NO) in cattle with foot-and-mouth-diseases (FMD). Twenty Swiss Brown cattle aged between 24 and 48 months were used. Animals were divided into two groups as control (n=10) and FMD (n=10). Blood samples were collected from jugular vein and centrifuged. TOC and NO levels were found to be significantly higher in FMD group compared to those of control group. However, no significant differences were present in TAC levels between FMD and control groups. It was concluded that FMD increases serum NO levels and TOC, but do not affect TAC in cattle. Keywords: Cattle, Foot-and -Mouth Disease, Oxidative stress, Nitric oxide Şap Hastalıklı Sığırlarda Nitrik Oksit Düzeyi, Total Antioksidan ve Oksidan Kapasite ÖzetÇalışmanın amacı şap hastalığına (FMD) yakalanmış sığırlarda total antioksidan (TAC), oksidan kapasite (TOC) ve nitrik oksit (NO) seviyelerini araştırmaktır. Araştırmada, yaşları 24 ve 48 ay arasında olan 20 Montofon ırkı sığır kullanıldı. Hayvanlar kontrol (n=10) ve şap hastalıklı (n=10) olmak üzere iki gruba ayrıldı. Kan örnekleri Vena jugularis' ten alınarak santrifuj edildi. Şaplı hayvanlarda TOC ve NO seviyeleri kontrol grubundaki hayvanlara göre önemli düzeyde yüksekti. TAC seviyesinde ise şap hastalıklı ve kontrol grubu arasında önemli bir değişiklik saptanmadı. Sonuç olarak şap hastalığının sığırlarda serum NO ve TOC seviyesini artırırken, TAC seviyesini etkilemediği belirlendi.

Kısa Süreli PRID Uygulanan Düvelerde Bazı Oksidatif Stres Parametrelerinin Değerlendirilmesi

“…A study conducted by Bozukluhan et al [9] found that serum NO levels in healthy animals (control group) were lower than those of diseased animals. In our study, the PRID was administered to healthy animals and because the device caused stress to the animal, we found that NO levels rose after the procedure.…”

“…Nitric oxide (NO) plays a role in many of the body's physiological functions and is produced by nitric oxide synthesis. NO is mostly produced by macrophage, neutrophil and mast cells [2,8,9] . Studies performed on cattle have reported that OS factors increase when the animals become sick [9][10][11] .…”

“…NO is mostly produced by macrophage, neutrophil and mast cells [2,8,9] . Studies performed on cattle have reported that OS factors increase when the animals become sick [9][10][11] . Other studies have shown that the level of OS factors increases in cases of retentio secundinarum, mastitis, subclinical mastitis, udder edema, anestrus, repeat breeders, follicular cysts and metabolic diseases [1,7,[12][13][14][15] .…”

Şap Hastalıklı Boğalarda Serum ve Salya Sialik Asit ve Oksidatif Stres Parametrelerindeki Değişiklikler

“…It is known that NO plays an important role in the primary defense mechanism against several pathogens; bacteria, viruses and parasites and NO production to be induced by various viruses which inhibit virus replication in vivo and [35][36][37] . Increased NO, a gaseous free radical, in this study is in agreement with the study of cellular elements such as lipopolysaccharide and glycolipoprotein, have been reported to activate leukocytes and stimulate the production of pro-inflammatory cytokines which induces production of NO through activation of inducible nitric oxide synthase (iNOS) [38][39][40][41][42] .…”

Nitric Oxide Synthase and Nitric Oxide Involvement in Different Toxicities

“…In recent studies in our laboratory and others, it has been reported that the amount of NO can change in response to various toxicity conditions [12][13][14][15][16][17][18] which are closely associated with animal and human disease conditions. In this chapter, we mention NO 3 − and NO 2 − , the molecules which are naturally found in foods as NO sources, agricultural activities such as the use of artificial fertilizers, polluted water, curing process to give a natural smell and taste to meat.…”