NIR SERS detection of immune reaction on gold colloid particles without bound/free antigen separation

Dou, Xiaoming; Yamaguchi, Yoshihiro; Yamamoto, Hiroyoshi; Doi, S.; Ozaki, Yukihiro

doi:10.1002/(sici)1097-4555(199808)29:8<739::aid-jrs289>3.0.co;2-s

Cited by 34 publications

(11 citation statements)

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“…While the silver staining method can give strongly enhanced Raman signals, the technique itself is somewhat complicated due to the larger number of functionalization steps. In another example, Dou et al (1998) proposed the use of nearinfrared (NIR) SERS detection of anti-mouse IgG on Au nanoparticles regardless of binding between antibodies and antigens. Unlike the general immunoassays which require procedures for the separation of bound and free antigens, the antigen here was minimally involved in yielding a SERS signal, owing to the distancing of the Au nanoparticles from the antigen.…”

Section: Immunoassay-based Detectionmentioning

confidence: 99%

Surface enhanced Raman spectroscopy and its application to molecular and cellular analysis

Huh

Chung

Erickson

2009

Microfluid Nanofluid

199

129

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In this paper, we review the state-of-the-art in surface-enhanced Raman scattering (SERS) based optical detection techniques with an application focus on cancer diagnostics. As we describe herein, SERS has several analytical, biological and engineering advantages over other methods including extremely high sensitivity, inherent molecular specificity of unlabeled targets, and narrow spectral bands. We review advances in both in vitro and in vivo applications of SERS and examine how technical issues with the technology are being addressed. A special technology focus is given to emerging optofluidic devices which aim to merge microfluidic and optical detection technologies into simple packages. We conclude with a brief discussion of some of the emerging challenges in the field and some of the approaches that are likely to enhance their application.

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Section: Immunoassay-based Detectionmentioning

confidence: 99%

Surface enhanced Raman spectroscopy and its application to molecular and cellular analysis

Huh

Chung

Erickson

2009

Microfluid Nanofluid

199

129

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“…22 ' 31 In this review we discuss three examples of biological quantitative assay by SERS selected from our recent studies. 27,30,31 Two of them are concerned with "indirect SERS method" which indirectly analyzes the object by observing SERS spectra of another compound. 27,30 By use of the idea of indirect SERS method, we increased markedly the sensitivity of SERS in enzyme immunoassay (see 3.2).…”

Section: Biological Quantitative Assaymentioning

confidence: 99%

“…We have demonstrated that near-infrared (NIR) SERS spectroscopy holds considerable promise in detecting the immune reaction on the gold on colloid particles without any procedure for the B/F separation. 31 Figure 20 Figure 21 shows a N1R-SERS spectrum of anti-mouse IgG adsorbed on the gold colloid particles. 31 The spectrum has an appearance fairly different from that of a typical protein Raman spectrum.…”

Section: Separation By Near-infrared Surface-enhanced Raman Scatteringmentioning

confidence: 99%

Surface-Enchanced Raman Scattering of Biological Molecules on Metal Colloids: Basic Studies and Applications to Quantitative Assay

Dou¹,

Ozaki²

1999

Reviews in Analytical Chemistry

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“…[15][16][17][18][19][20][21][22][23][24] In the cases of fiber optic nanosensors, tapered fiber optic tips that have been functionalized with various bioreceptor molecules have been developed, that are capable of monitoring specific intracellular species such as nitrite-chloride-, 25 benzo[a]pyrene tetrol, 5 pH, 26 etc. However, these fiber optic nanosensors are relatively large and therefore can result in stress or damage to the cells, making their use limited.…”

Section: Introductionmentioning

confidence: 99%

Implantable SERS nanosensors for pre-symptomatic detection of BW agents

Sun

Alexander

et al. 2005

SPIE Proceedings

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The early detection of biological warfare (BW) agents before any symptoms are present is critical for saving lives and reducing cost of therapy. Protein expression in T-cells represents one of the earliest detectable cellular signaling events to occur in response to the exposure to various toxins or BW agents. In order to fully understand a cellular response to a particular BW agent, it is often necessary to monitor the expression of specific proteins. Therefore, we have developed a novel class of surface enhanced Raman scattering (SERS) immuno-nanosensors for the real-time monitoring of protein expression within individual living cells.In this work, we have developed and optimized novel nanosphere-based silver coated SERS nanosensors for the detection of proteins at cellular levels. SERS nanosensors were optimized in terms of nanosphere size, silver coating methods, number of silver layers, antibody binding and affinity. These nanosensors are capable of being inserted into individual cells and non-invasively positioned to the sub-cellular location of interest using optical tweezers. They were constructed from monodisperse silica nanospheres. These nanospheres were condensed from tetraalkoxysilanes in an alcoholic solution of water and ammonia. Accurate control of the silica nanospheres' diameter was achieved by varying the reaction conditions. Nanosphere-based SERS immuno-nanosensors were then prepared by depositing multiple layers of silver on silica spheres, followed by binding of the antibody of interest to the silver. In binding the antibodies, different cross linker agents were characterized and compared. On one end, each of these cross linker agents contained sulfur or isothiocyanate groups which bound to the silver surface, while the other end contained a carboxylic or primary amine group which reacted readily with the antibodies. In order to improve sensitivity of these nanosensors, optimal silver surface coverage with crosslinkers was determined. Following binding of antibodies, evaluation of the nanosensors was performed by monitoring the SERS spectra of the nanosensors prior to and following exposure to the antigen of interest. These results showed reproducible differences in the SERS spectra upon exposure to the antigens confirming their ability to monitor trace amounts of antigen. In particular, these SERS-based nanosensors were shown successfully detect human insulin at trace levels.

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NIR SERS detection of immune reaction on gold colloid particles without bound/free antigen separation

Cited by 34 publications

References 0 publications

Surface enhanced Raman spectroscopy and its application to molecular and cellular analysis

Surface enhanced Raman spectroscopy and its application to molecular and cellular analysis

Surface-Enchanced Raman Scattering of Biological Molecules on Metal Colloids: Basic Studies and Applications to Quantitative Assay

Implantable SERS nanosensors for pre-symptomatic detection of BW agents

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