NIR reflectance spectroscopic analysis of the FA composition in sesame (Sesamum indicum L.) seeds

Abstract

The feasibility of using NIR reflectance spectroscopy to estimate the FA composition of sesame seed (Sesamum indicum L.) samples from the National Institute of Crop Science of Japan and from Myanmar was examined. Multiple linear-regression analyses of NIR spectral data and chemical data for whole seeds were carried out to develop calibration equations for predicting the proportion of each of the four major FA in sesame seeds from the total FA composition. The SE of prediction (SEP) was 0.616% for palmitic acid, 0.348% for stearic acid, 1.051% for oleic acid, and 0.826% for linoleic acid. This NIR method provides a simple, rapid, and nondestructive means of estimating the FA composition of sesame seeds for breeding selection, regardless of the color of the sesame seed coats. However, the proportions of palmitic and stearic acids could not be reliably measured because their SEP were almost as great as the SD of their concentrations in the set of prediction samples. The relationship between NIR spectral patterns and the FA composition of sesame seeds also was examined. The correlation coefficient calculated for the standardized second-derivative NIR spectral readings at 1708 nm and the percentages of linoleic acid was −0.830. A rough estimate of the proportion of linoleic acid in the total FA composition of sesame seeds could be obtained even with single sesame seeds, except for those with a black coat, based on NIR spectral pattern analysis using the wavelength assignments of linoleic acid.

Nondestructive Near-Infrared Reflectance Spectroscopy of Sesame (Sesamum indicum L.) Components by Single Seed Analysis

“…The samples and the methods of chemical measurements were the same as those used in the previous report (Sato et al, 2003;Sato et al, 2004).…”

“…The seeds need to be analyzed nondestructively for use in breeding and cultivation after the analysis and selection. We previously reported that the amounts of the major constituents and the fatty acid (FA) composition in plural sesame seeds could be successfully estimated by simple, rapid and nondestructive analysis using near infrared (NIR) spectroscopy (Sato et al, 2003;Sato et al, 2004). In this report, the nondestructive evaluation of sesame composition by using NIR spectroscopy was investigated for analysis of a small sample, i.e., single seed.…”

Nondestructive Near-Infrared Refl ectance Spectroscopic Analyses of the Major Constituents of Sesame(Sesamum indicumL.) Whole Seeds with Different Coat Color

“…These samples were sent to the National Agricultural Research Center for Kyushu Okinawa Region (KONARC, Kumamoto, Japan) for chemical and NIR analyses. The samples were the same as those used in the previous report (Sato et al, 2003).…”

“…NIR is also used for analyzing the fatty acid composition in sesame seeds (Sato et al, 2003) and for identifying their geographic origin by their contents (Kwon et al, 1998). The major constituents are important indices of seed quality for nutritional classifi cation, and their quantifi cation by NIR analyses would be valuable.…”

In Situ Estimation of the Constituents of Green Soybean (Edamame) Pod using Near-Infrared Transmission Spectroscopy

“…Perez-Vich et al (1998) carried out a methodological study on the seed oil content and fatty acid composition of sunflower, and found that the accuracy when intact seeds were used was less than that for meal, but was sufficiently reliable for prescreening. Sato et al examined the feasibility of NIR spectroscopy for estimating sesame seed fatty acid (FA) composition, and reported that it was reasonably accurate for breeding purposes (Sato et al, 2003). Golebiowski (2004) and Golebiowski et al (2005) used NIR spectroscopy to investigate the oil in intact canola seed and discussed the attributes of the spectrum and the relationship between the principal components and the oil content.…”