Nigella sativa conserved hippocampal oxidative and neurogenic activities to salvage neuro-cognitive integrities in chlorpyrifos insult

Imam, Aminu; Lawal, A.Z.; Oyewole, Lukuman Aboyeji; Ajibola, Musa Iyiola; Williams, Victoria; Chengetanai, Samson; Shittu, ST; Ajao, Moyosore S.

doi:10.1016/j.sciaf.2018.e00008

Cited by 7 publications

(8 citation statements)

References 56 publications

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“…32 Oral ingestion of CPF caused several histopathological changes in the cerebellum, and these can be supported by other studies conducted on CPF and other pesticides in other regions of the brain. 15 Goel et al, administered 13.5 mg/kg chlorpyrifos to rats and it caused biochemical and histopathological changes such as necrosis, vacuolization and dilatation of sinusoids, and these they suggested can be associated with the surged generation of ROS in the exposed rats. 33 In this study, treatment with CPF showed widened spaces, altered distribution of cell masses and dilatation of sinusoids.…”

Section: Discussionmentioning

confidence: 99%

“…Group 1 (control) -were given normal saline (1 ml/ kg orally) daily Group 2-were given CPF (14.9 mg/kg orally) daily Group 3-were given CPF (14.9 mg/kg orally) plus NSO (1 ml/kg orally) daily 14 Group 4-were given NSO (1 ml/kg orally) daily 14,15,18,19,21,22 The experiments were all conducted in the morning (between 07:00 and 09:00 am), and treatments with the experimental substances spanned a period of fourteen consecutive days.…”

Section: Treatment Schedulementioning

confidence: 99%

“…12,13 The oil of Nigella sativa (NSO), is one of the forms in which the pharmacological potency of Nigella sativa has been widely examined. The beneficial qualities of NSO include antioxidant, 10,14,15 anti-inflammatory, 16,17 neuroprotective, 14,15,18 immunomodulatory, 19 efficacy against neurodegenerative disease 20 and memoryenhancing effects. 21,22 In the present study we report the possible mitigating role of NSO in CPF-induced oxidative damage, neurogenic loss, cholinesterase dysfunction in the cerebellum and the resulting implications on motor function and neuronal integrities in the cerebellum and the primary motor cortex, using combined behavioural, biochemical and histochemical methods.…”

Section: Introductionmentioning

confidence: 99%

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Chlorpyrifos impaired cerebellar oxidative and cholinesterase activities in rats: Mitigating efficacy of Nigella sativa Oil

et al. 2021

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Background: Motor dysfunctions are some of the characteristic symptoms of organophosphate (OP) poisoning and they have been associated with decreased levels of cholinesterase inhibition within motor areas of the brain. Objectives: The current study aims to investigate the potential neuroprotective effects of Nigella sativa oil (NSO) in alleviating chlorpyrifos (CPF) induced toxicity in the cerebella and motor cortices of the rat brains using combined behavioural, biochemical and histochemical methods. Methods: Thirty-two rats were randomly divided into four groups (eight rats per group), exposed to 1ml/kg of normal saline, 14.9 mg/kg of CPF, 14.9 mg/kg of CPF plus 1ml/kg of NSO and 1ml/kg of NSO respectively for 14 consecutive days. The rats were each exposed to a single trial of the Open Field Test (OFT) on day 13 of the experiment. This experimental test measured locomotor activity levels (line crossing frequency (LCF)) and exploratory (rearing frequency (RF)) activities in the rats studied. The rats were euthanized on day 15 of the experiment and the brains were subsequently excised. The cerebella cortices of five brains were removed and homogenised to analyse for total reactive oxygen species (ROS), nitric oxide (NO) levels and acetylcholinesterase (AChE) activity. The motor and cerebella cortices from three other brains in each group were processed for histology (Nissl stain) and proliferative activity (Ki67 immunohistochemistry). Results: Rats exposed to CPF experienced a significant increase in cerebella NO and ROS levels, depletion in AChE activity, neurogenic cells loss and subsequent reduction in locomotor and exploratory behaviours respectively (LCF and RF). However, interventional treatment with NSO depleted markers of oxidative damage (NO and ROS), reduced AChE inhibition, preserved neurogenic (Ki67) cells distribution and motor functions. Conclusion: These results demonstrate the potential efficacy of NSO in OP poisoning and the roles of neurogenic and oxidative functions in the pathophysiology and treatment of motor dysfunction in OP neurotoxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Treatment Schedulementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chlorpyrifos impaired cerebellar oxidative and cholinesterase activities in rats: Mitigating efficacy of Nigella sativa Oil

et al. 2021

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“…Several studies revealed that Nigella sativa has pharmacological properties among them are the neuroprotective improvement of neurodegenerative illnesses, as well as memory enhancement ( Imam et al, 2018 ). A previous study by Yuste et al (2015) evaluated the Nigella sativa protective effect against Chlopyrifos which has been linked to the pathophysiology of many neurodegenerative illnesses.…”

Section: Discussionmentioning

confidence: 99%

Green synthesis of silver nanoparticles by Nigella sativa extract alleviates diabetic neuropathy through anti-inflammatory and antioxidant effects

Alkhalaf

Hussein

Hamza

2020

Saudi Journal of Biological Sciences

112

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Green synthesis of silver nanoparticles has gained great interest among scientists. In view of this data, we conducted this study to identify the ameliorative effect of green synthesis of silver nanoparticles using Nigella sativa extract in diabetic neuropathy induced experimentally. In this study, 50 adult male albino rats were used and they were randomly divided into five groups; the first group was the healthy control group, the second group were the diabetic neuropathy diabetic neuropathy induced, Groups (3-6) diabetic neuropathy induced group and treated with silver nanoparticles, Nigella sativa extract and green synthesized silver nanoparticles using Nigella sativa extract respectively. Biochemical parameters including diabetic, inflammatory and antioxidant biomarkers were evaluated. Brain histopathology was also performed. Results revealed substantial rise in glucose, AGE, aldose reductase with insulin reduction in diabetic neuropathy induced group as compared to healthy control. Also, inflammatory markers increased significantly in diabetic neuropathy induced group. A remarkable change in oxidative status was observed in the same group. Furthermore, significant decline in nitrotyrosin level was observed. Regarding gene expression, we found significant down regulation in brain TKr A accompanied by upregulation of nerve growth factor in diabetic neuropathy group comparing with healthy control. Several treatments for diabetic neuropathy remarkably ameliorate all the investigated biomarkers. Histological findings are greatly relied on for the results achieved in this study. Therefore, it can be established that green synthesis of silver nanoparticles in combination with Nigella sativa extract could be a newly neuroprotective agents against inflammation and oxidative stress characterizing diabetic neuropathy through their antidiabetic, anti-inflammatory and anti-oxidants effects.

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“…The role of N. sativa and its components as promising neuropharmacological agents (Beheshti et al, 2016) in facitilating learning and memory have been recently reviewed (Sahak et al, 2016). In rats, 1 ml/kg of N. sativa oil for 14 days depleted reactive oxygen species (ROS)/NO levels, improved neurogenic proteins, acetylcholinesterase (AChE) activities and neuro-cognitive markers depletions in chlopyrifos exposure (Imam et al, 2018). Moreover, N. sativa oil improved neurocognicives indices such as Morris water maze.…”

Section: Neuroprotectionmentioning

confidence: 99%

Nigella Plants – Traditional Uses, Bioactive Phytoconstituents, Preclinical and Clinical Studies

et al. 2021

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Nigella is a small genus of the family Ranunculaceae, which includes some popular species due to their culinary and medicinal properties, especially in Eastern Europe, Middle East, Western, and Central Asia. Therefore, this review covers the traditional uses and phytochemical composition of Nigella and, in particular, Nigella sativa. The pharmacological studies reported in vitro, in vivo, and in humans have also been reviewed. One of the main strength of the use of Nigella is that the seeds are rich in the omega-6 fatty acid linoleic acid and provide an extra-source of dietary phytochemicals, including the bioactive thymoquinone, and characteristics saponins, alkaloids, and flavonoids. Among Nigella species, N. sativa L. is the most studied plant from the genus. Due to the phytochemical composition and pharmacological properties, the seed and seed oil from this plant can be considered as good candidates to formulate functional ingredients on the basis of folklore and scientific knowledge. Nonetheless, the main limations are that more studies, especially, clinical trials are required to standardize the results, e.g. to establish active molecules, dosage, chemical profile, long-term effects and impact of cooking/incorporation into foods.

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Nigella sativa conserved hippocampal oxidative and neurogenic activities to salvage neuro-cognitive integrities in chlorpyrifos insult

Cited by 7 publications

References 56 publications

Chlorpyrifos impaired cerebellar oxidative and cholinesterase activities in rats: Mitigating efficacy of Nigella sativa Oil

Chlorpyrifos impaired cerebellar oxidative and cholinesterase activities in rats: Mitigating efficacy of Nigella sativa Oil

Green synthesis of silver nanoparticles by Nigella sativa extract alleviates diabetic neuropathy through anti-inflammatory and antioxidant effects

Nigella Plants – Traditional Uses, Bioactive Phytoconstituents, Preclinical and Clinical Studies

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