Nickel toxicity in P. lividus embryos: Dose dependent effects and gene expression analysis

Bonaventura, Rosa; Zito, Francesca; Chiaramonte, Marco; Costa, Caterina; Russo, Roberta

doi:10.1016/j.marenvres.2018.05.002

Cited by 23 publications

(17 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over the last 10 years it has been shown that alteration of ChE, PKS and GST activity may cause morphological alterations of the spicules, similar to those shown in Figure 4 [ [50][51][52]. In fact, skeletal malformations and delayed larvae correlated to significant down-regulation of PKS gene was recently found after a 24-and 48-h exposure of P. lividus eggs to nickel [53]. Similarly, CYP-2AI, hsp70 and hsp90 are involved in defense against chemical stressors, and their alteration is able to impact the gastrulation process [54][55][56].…”

Section: Discussionmentioning

confidence: 61%

PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos

Albarano

Zupo

Guida

et al. 2021

IJMS

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) represent the most common pollutants in the marine sediments. Previous investigations demonstrated short-term sublethal effects of sediments polluted with both contaminants on the sea urchin Paracentrotus lividus after 2 months of exposure in mesocosms. In particular, morphological malformations observed in P. lividus embryos deriving from adults exposed to PAHs and PCBs were explained at molecular levels by de novo transcriptome assembly and real-time qPCR, leading to the identification of several differentially expressed genes involved in key physiological processes. Here, we extensively explored the genes involved in the response of the sea urchin P. lividus to PAHs and PCBs. Firstly, 25 new genes were identified and interactomic analysis revealed that they were functionally connected among them and to several genes previously defined as molecular targets of response to the two pollutants under analysis. The expression levels of these 25 genes were followed by Real Time qPCR, showing that almost all genes analyzed were affected by PAHs and PCBs. These findings represent an important further step in defining the impacts of slight concentrations of such contaminants on sea urchins and, more in general, on marine biota, increasing our knowledge of molecular targets involved in responses to environmental stressors.

show abstract

Section: Discussionmentioning

confidence: 61%

PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos

Albarano

Zupo

Guida

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Studies have investigated the effects of various environmental factors, such as light (Belleza et al, 2012), temperature (Byrne et al, 2009;Abdul Wahab et al, 2016;Mejía-Gutiérrez et al, 2019;Ding et al, 2020), and metal content in a waterbody (Bielmyer et al, 2005;Bonaventura et al, 2018;Di Natale et al, 2019), on the embryonic development and behavior of sea urchins. For light, studies have predominantly employed natural light and UV light in their experiments, and few of them have included light with different wavelengths in the experiments.…”

Section: Discussionmentioning

confidence: 99%

Embryo Development and Behavior in Sea Urchin (Tripneustes gratilla) Under Different Light Emitting Diodes Condition

Hsieh

et al. 2021

Front. Mar. Sci.

View full text Add to dashboard Cite

This study aims to evaluate the effect of light-emitting diodes (LEDs) of different wavelengths on the embryonic development, covering behavior, righting behavior, and phototaxis of collector urchins (Tripneustes gratilla). The collector urchins were divided into three groups according to the type of LED illumination they received: full-spectrum (400–750 nm wavelength), red light (630 nm), or blue light (450 nm). The results of the embryonic development experiment indicated that the blue LED group had the highest proportion of embryos reaching the prism stage at the 24th hour and the highest proportion of embryos entering the 4-arm pluteus stage, but it also had the highest death rate at the 48th hour. The full-spectrum and red LED groups exhibited similar speeds of embryonic development. In the experiment on covering behavior performed on adult urchins, our findings indicated that the blue LED group gripped the most acrylic sheets for cover, exhibiting the most covering behavior, followed by the full-spectrum group and then the red LED group. Moreover, behavior varied with coloration, as collector urchins with a lower level of melanin exhibited more covering behavior than those with a higher melanin level. In addition, the righting behavior experiments demonstrated that the blue LED group spent the longest time righting themselves. It is possible that the relatively strong stimulation from the blue LED illumination led to a higher level of stress in the collector urchins and hence slowed their righting. The phototaxis experiment revealed the most significant negative phototactic response in collector urchins when they were under the blue LED light, followed by the full-spectrum light; the red LED light did not induce any positive or negative phototactic response in the collector urchins. This experimental result verified collector urchins’ high sensitivity to and dislike of the blue LED light. The study results confirmed that the blue LED light environment accelerated the embryonic development of collector urchins; however, the relatively strong stimulation from that light also caused them to engage in covering behavior or move away from the light. These results indicate that short-wavelength irradiation significantly affects the embryonic development and behavior pattern of this species.

show abstract

“…In the last years, several studies were performed on the sea urchin innate immune system that reinforced the notion of its use as a model for immunological purpose, due to its evolutionary proximity to chordates and vertebrates (Branco et al., ; Rast, Smith, Loza‐Coll, Hibino, & Litman, ). Moreover, P. lividus was extensively used as a biological indicator of local pollution and of stress (Bonaventura, Zito, Chiaramonte, Costa, & Russo, ; Russo et al., ; Scanu et al., ).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary conserved pathway of the innate immune response after a viral insult in Paracentrotus lividus sea urchin

Chiaramonte

Arizza

Russo

2019

Int J Immunogenetics

Self Cite

View full text Add to dashboard Cite

Despite the apparent simplicity of the body organization of echinoderms, their immune system is competent to perform a complex innate immune response, which is far from being well understood. The echinoderms represent the most advanced invertebrates that form a bridge with the primitive chordates. In fact, they possess numerous receptors and effectors that are used to obtain a fast immune response. After an infection, the humoral and cellular immune response determines a network in which the main protagonists are membrane and endosomal receptors. The recognition of nonself molecules by specific membrane receptors triggers the immune response, stimulating consecutive intracellular events. We have previously shown how the polyinosinic–polycytidylic acid (polyI:C) that mimics a viral infection is able to induce an immune response in the sea urchin Paracentrotus lividus immune cells. It activates a specific membrane receptor belonging to the Toll‐like receptor (TLR) family. Here, we show the activated expression pattern of some genes involved in the downstream cascade of TLR signalling pathway, such as Pl‐Tbk and Pl‐Irf, whose partial sequence was isolated from P. lividus immune cells. Their mRNA expression increases consequentially to the polyI:C stimulation and in a temporal way. In addition, we analysed the expression of Pl‐NF‐kB and we found that its upregulation was time‐dependent, preceding Pl‐Tbk and Pl‐Irf increase. Protein analysis showed that also some cytokine (TNF‐α and IL‐1α) expression increased after polyI:C insult. Therefore, the purpose of this study was to discover the molecular mechanisms of the innate defence strategies, similarly to vertebrates, implemented by the sea urchins in order to cope with viral infection challenge.

show abstract

Nickel toxicity in P. lividus embryos: Dose dependent effects and gene expression analysis

Cited by 23 publications

References 50 publications

PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos

PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos

Embryo Development and Behavior in Sea Urchin (Tripneustes gratilla) Under Different Light Emitting Diodes Condition

Evolutionary conserved pathway of the innate immune response after a viral insult in Paracentrotus lividus sea urchin

Contact Info

Product

Resources

About