NGS Reveals Molecular Pathways Affected by Obesity and Weight Loss-Related Changes in miRNA Levels in Adipose Tissue

Kuryłowicz, Alina; Wicik, Zofia; Owczarz, Magdalena; Jonas, Marta; Kotlarek, Marta; Świerniak, Michał; Lisik, Wojciech; Jonas, Maurycy; Noszczyk, Bartłomiej; Puzianowska-Kuźnicka, Monika

doi:10.3390/ijms19010066

Cited by 40 publications

(76 citation statements)

References 42 publications

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“…Fifty-eight miRNAs were downregulated post surgery, using RNA sequencing analysis in unpaired samples (n = 10 each time point) (12). In line with our observations, miR-223-3p was downregulated (12). However, caveats of comparing our findings with those of the RNA sequencing analysis are that they used unpaired samples and did not specify the bariatric surgery procedure.…”

Section: Discussionsupporting

confidence: 82%

MicroRNA Profiling in Adipose Before and After Weight Loss Highlights the Role of miR‐223‐3p and the NLRP3 Inflammasome

Macartney-Coxson

Danielson

Clapham

et al. 2020

Obesity

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Objective Adipose tissue plays a key role in obesity‐related metabolic dysfunction. MicroRNA (miRNA) are gene regulatory molecules involved in intercellular and inter‐organ communication. It was hypothesized that miRNA levels in adipose tissue would change after gastric bypass surgery and that this would provide insights into their role in obesity‐induced metabolic dysregulation. Methods miRNA profiling (Affymetrix GeneChip miRNA 2.0 Array) of omental and subcutaneous adipose (n = 15 females) before and after gastric bypass surgery was performed. Results One omental and thirteen subcutaneous adipose miRNAs were significantly differentially expressed after gastric bypass, including downregulation of miR‐223‐3p and its antisense relative miR‐223‐5p in both adipose tissues. mRNA levels of miR‐223‐3p targets NLRP3 and GLUT4 were decreased and increased, respectively, following gastric bypass in both adipose tissues. Significantly more NLRP3 protein was observed in omental adipose after gastric bypass (P = 0.02). Significant hypomethlyation of NLRP3 and hypermethylation of miR‐223 were observed in both adipose tissues after gastric bypass. In subcutaneous adipose, significant correlations were observed between both miR‐223‐3p and miR‐223‐5p and glucose and between NLRP3 mRNA and protein levels and blood lipids. Conclusions This is the first report detailing genome‐wide miRNA profiling of omental adipose before and after gastric bypass, and it further highlights the association of miR‐223‐3p and the NLRP3 inflammasome with obesity.

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Section: Discussionsupporting

confidence: 82%

MicroRNA Profiling in Adipose Before and After Weight Loss Highlights the Role of miR‐223‐3p and the NLRP3 Inflammasome

Macartney-Coxson

Danielson

Clapham

et al. 2020

Obesity

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“…This method has been widely used in chicken [18], ducks [19], pigs [20], and cattle [8]. Especially in the study of adipose tissue and adipocytes, RNA-seq was used to identify differentially expressed RNAs including mRNA [21], miRNAs [22], and LncRNAs [23]. In this work, we applied RNA-seq to analyze the adipose tissue transcriptome in Qaidamford cattle, cattle-yak, and Angus cattle, and identified 4167 differentially expressed genes (Angus vs. cattle-yak), 3269 differentially expressed genes (Angus vs. Qaidamford cattle), and 154 differentially expressed genes (cattle-yak vs. Qaidamford cattle).…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Analysis Identifies Differentially Expressed Genes in Subcutaneous Adipose Tissue in Qaidaford Cattle, Cattle-Yak, and Angus Cattle

Huang

Song

et al. 2019

Animals

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In the beef industry, fat tissue is closely related to meat quality. In this study, high-throughput RNA sequencing was utilized for adipose tissue transcriptome analysis between cattle-yak, Qaidamford cattle, and Angus cattle. The screening and identification of differentially expressed genes (DEGs) between different breeds of cattle would facilitate cattle breeding. Compared to Angus cattle adipose tissue, a total of 4167 DEGs were identified in cattle-yak adipose tissue and 3269 DEGs were identified in Qaidamford cattle adipose tissue. Considering cattle-yak as a control group, 154 DEGs were identified in Qaidamford cattle adipose tissue. GO analysis indicated the significant enrichment of some DEGs related to lipid metabolism. The KEGG pathway database was also used to map DEGs and revealed that most annotated genes were involved in ECM-receptor interaction and the PI3K-Akt signal pathway, which are closely related to cell metabolism. Eight selected DEGs related to adipose tissue development or metabolism were verified by RT-qPCR, indicating the reliability of the RNA-seq data. The results of this comparative transcriptome analysis of adipose tissue and screening DEGs suggest several candidates for further investigations of meat quality in different cattle breeds.

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“…Recently there has been a rapidly growing interest in the role of miRNAs in fat cell development and obesity, and there is also evidence that miRNA plays a role in the regulation of sirt activity [18,23,46,72]. Therefore, one can assume that strategies based on modifying the action of sirts by specific miRNAs may also be useful in treating obesity.…”

Section: Sirtuins As Targets For Obesity Treatmentmentioning

confidence: 99%

Role of Sirtuins in Adipose Tissue Development and Metabolism

Kuryłowicz¹

2019

Adipose Tissue - An Update

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Sirtuins (silent information regulators, sirts) via modification of histones, as well as transcription factors and co-regulators, control expression of other genes, particularly those involved in the organism response to stress. Detection of sirtuin expression in adipocytes initiated interest in their role in adipose tissue development and metabolism. This chapter presents how sirtuins control the critical steps of preadipocytes' differentiation and proliferation, as well as the process of adipose tissue browning. Moreover, it shows in vitro and in vivo data proving that sirtuins are involved in the regulation of lipogenesis, lipolysis, and secretory activity of adipose tissue. Due to all these reasons, sirtuins may constitute potential targets in the treatment of obesity and related complications. 1 2. A short review of the sirt system The sirts are highly conserved regulatory proteins present almost in all species. Initially, they have been identified as class III histone deacetylases, nicotinamide adenine dinucleotide (NAD)-dependent enzymes responsible for the removal of acetyl groups from lysine residues in proteins, while some members of this family act also as mono-ADP-ribosyltransferases. Since acetylation and deacetylation are essential mechanisms of posttranslational modifications of proteins determining their activity, sirts were found to be involved in the regulation of distinct cellular pathways including, among others, those related to cell survival, apoptosis, inflammatory and stress responses, as well as lipid and glucose homeostases [2].In human, seven sirt genes (sirts) have been identified that encode seven sirt enzymes of different structure, cellular localization, and tissue expression. All of them share a common conserved catalytic core region consisting of approximately 275 amino acids, forming a Rossmann fold domain (characteristic of NAD + /NADH-binding proteins) and a zinc-binding domain connected by several loops [2]. Outside the catalytic core, sirt enzymes possess variable N-and C-terminal regions that decide about their enzymatic activities, binding partners and substrates, as well as subcellular localization [3]. sirt1, sirt6, and sirt7 localize predominantly in the nucleus where via modifications of transcription factors, cofactors, and histones they participate in the regulation of energy metabolism, stress and inflammatory responses, DNA repair (sirt1 and sirt6), and rDNA transcription (sirt7) [4]. sirt2 is a cytoplasmic sirtuin and plays a role in cell cycle control [5]. sirt3 can be found in mitochondria where it takes part in the regulation of enzymes involved, e.g., in glycolysis, fatty acid (FA) oxidation, ketone body synthesis, and the catabolism of amino acids as well as of apoptosis and oxidative stress pathways. This sirtuin also has as a nuclear full-length form (FL-sirt3) that is processed to the short mitochondrial form. Therefore, sirt3 may regulate cellular metabolism both at the transcriptional and posttranscriptional levels. sirt4 is also localized in mitochondria...

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NGS Reveals Molecular Pathways Affected by Obesity and Weight Loss-Related Changes in miRNA Levels in Adipose Tissue

Cited by 40 publications

References 42 publications

MicroRNA Profiling in Adipose Before and After Weight Loss Highlights the Role of miR‐223‐3p and the NLRP3 Inflammasome

MicroRNA Profiling in Adipose Before and After Weight Loss Highlights the Role of miR‐223‐3p and the NLRP3 Inflammasome

RNA-Seq Analysis Identifies Differentially Expressed Genes in Subcutaneous Adipose Tissue in Qaidaford Cattle, Cattle-Yak, and Angus Cattle

Role of Sirtuins in Adipose Tissue Development and Metabolism

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