NF1, Sp1 and HSF1 are synergistically involved in sulfide-induced sqr activation in echiuran worm Urechis unicinctus

Liu, Xiaolong; Qin, Zhenkui; Li, Xueyu; Ma, Xiaoyu; Gao, Beibei; Zhang, Zhifeng

doi:10.1016/j.aquatox.2016.04.002

Cited by 9 publications

(5 citation statements)

References 23 publications

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“…11 Furthermore, it has been reported that abnormal expression of DR4 is also correlated with the prognosis of colorectal cancer 10 and lung squamous carcinoma cell patients. 25 Taken together, we speculate herein that NF1 might activate the membrane related DR4, and ultimately promote apoptosis of colorectal cancer cells. 23 Herein, we found that rs13278062 GT/TT genotypes increased DR4 expression by affecting the binding affinity of Sp1 and NF1 to the DR4 promoter in colorectal cancer cells.…”

Section: Discussionmentioning

confidence: 55%

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DR4 mediates the progression, invasion, metastasis and survival of colorectal cancer through the Sp1/NF1 switch axis on genomic locus

Meng

Zhang

et al. 2018

Intl Journal of Cancer

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The single nucleotide polymorphism (SNP), -397G > T (rs13278062) polymorphism, in the promoter of Death Receptor 4 (DR4) had been reported to be associated with a significantly increased risk for bladder cancer. However, the association of this SNP with the risk of colorectal cancer has not been reported. In this study, we performed a case-control study in 1,078 colorectal cancer patients and 1,175 matched healthy controls to evaluate the association of the potential functional genetic variants in DR4 with risk and survival of colorectal cancer. PCR-TaqMan were used to genotype the rs13278062, rs1000294 and rs2235126 polymorphisms. We found that subjects carrying the rs13278062 GT/TT genotypes had a significantly lower risk and increased survival time when compared to the GG genotype. We also constructed the rs13278062 GT/TT genotype in SW480 and SW620 cells (rs13278062 is GG in both cell lines) with the CRISPR/Cas9 system. Flow cytometry experiments showed that the rs13278062 TT genotype promoted apoptosis in colorectal cancer cells. In vitro and in vivo experiments established that the rs13278062 G to T mutation inhibited carcinogenesis and metastasis of colorectal cancer. Chromatin immunoprecipitation (ChIP) assays revealed that the rs13278062 G > T polymorphism altered the binding affinity of the transcription factors Sp1/NF1 to the rs13278062 mutation region. Immunohistochemistry, western blot, and qPCR corroborated that the rs13278062 GT/TT genotypes increased the expression of DR4 protein in colorectal cancer tissues and cells. In conclusion, these findings indicate that DR4 mediated progression, invasion, metastasis and survival of colorectal cancer via the Sp1/NF1 switch axis on genomics locus.

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Section: Discussionmentioning

confidence: 55%

“…24 NF1, however, was reported as a transcriptional activator. 25 Taken together, we speculate herein that NF1 might activate the membrane related DR4, and ultimately promote apoptosis of colorectal cancer cells. Regretfully, we observed an opposite result in DR4 protein levels in followed TMAs ISH assay.…”

Section: Discussionmentioning

confidence: 55%

DR4 mediates the progression, invasion, metastasis and survival of colorectal cancer through the Sp1/NF1 switch axis on genomic locus

Meng

Zhang

et al. 2018

Intl Journal of Cancer

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“…U. unicinctus lives in U-shape burrows in the subtidal zone and has high nutritional and medicinal values, rapidly promoting its aquaculture [ 15 , 16 ]. Existing studies mainly focus on its nutritional components, medical value, and environmental tolerance [ 17 , 18 ]. However, there are few studies on U. unicinctus from the perspective of mechanics, which is the key to revealing the burrowing mechanisms in sands.…”

Section: Introductionmentioning

confidence: 99%

Radial Expansion Favors the Burrowing Behavior of Urechis unicinctus

Li,

Zhang,

Zhang

et al. 2023

Applied Bionics and Biomechanics

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Urechis unicinctus can utilize the ability of large deformation to advance in sands by radial expansion, just using a small force. However, the large deformation of U. unicinctus skin and the discrete nature of the sands make it hard to analyze this process quantitatively. In this study, we aim to uncover the burrowing mechanism of U. unicinctus in granular sediments by combining discrete and finite elements. We observe that U. unicinctus will expand radially at the head, and then the head will shrink to move forward. The radial expansion will collapse the sands and let them flow, making it easy to advance. U. unicinctus mainly relies on the skin’s large deformation and sufficient pressure to achieve radial expansion. Thus, we first establish the large deformation constitutive model of the skin. The stress–strain relationship can be expressed by the Yeoh model. Meanwhile, the pressure required for radial expansion is indirectly measured by the balloon experiment. To study the effect of radial expansion on the burrowing behavior, we use the finite element method–discrete element method (FEM–DEM) coupling model to simulate the expansion process of burrowing. The simulated pressure for radial expansion is very close to the experimental data, verifying the reliability of the simulation. The results show that the expansion can drastically reduce the pressure of sand particles on the head front face by 97.1% ± 0.6%, significantly decreasing the difficulty of burrowing. This unique underwater burrow method of U. unicinctus can provide new ideas for engineering burrowing devices in soft soil, especially for granular sediments.

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“…The Echiura worm Urechis unicinctus is a benthic organism inhabiting U-shaped burrows in the intertidal zone in China, Korea, and Japan, and is a delicious seafood with high nutritional value [ 28 ]. Previous researchers have revealed that U. unicinctus can metabolize and utilize environment sulfide, and exhibits a strong sulfide tolerance as well as surviving well in a sulfide-rich environment [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. To better understand the composition characteristics of the HSP superfamily and the mechanism of HSP function in the sulfide tolerance of burrowing animals, we identified members of the HSP superfamily according to our whole genome data of U. unicinctus , and analyzed the evolutionary relationship of HSPs by comparing the genome data with closely related species.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analyses of Heat Shock Protein Superfamily Provide New Insights on Adaptation to Sulfide-Rich Environments in Urechis unicinctus (Annelida, Echiura)

Liu

Qin

Wei

et al. 2022

IJMS

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The intertidal zone is a transitional area of the land-sea continuum, in which physical and chemical properties vary during the tidal cycle and highly toxic sulfides are rich in sediments due to the dynamic regimes. As a typical species thriving in this habitat, Urechis unicinctus presents strong sulfide tolerance and is expected to be a model species for sulfide stress research. Heat shock proteins (HSPs) consist of a large group of highly conserved molecular chaperones, which play important roles in stress responses. In this study, we systematically analyzed the composition and expression of HSPs in U. unicinctus. A total of eighty-six HSP genes from seven families were identified, in which two families, including sHSP and HSP70, showed moderate expansion, and this variation may be related to the benthic habitat of the intertidal zone. Furthermore, expression analysis revealed that almost all the HSP genes in U. unicinctus were significantly induced under sulfide stress, suggesting that they may be involved in sulfide stress response. Weighted gene co-expression network analysis (WGCNA) showed that 12 HSPs, including 5 sHSP and 4 HSP70 family genes, were highly correlated with the sulfide stress response which was distributed in steelblue and green modules. Our data indicate that HSPs, especially sHSP and HSP70 families, may play significant roles in response to sulfide stress in U. unicinctus. This systematic analysis provides valuable information for further understanding of the function of the HSP gene family for sulfide adaptation in U. unicinctus and contributes a better understanding of the species adaptation strategies of marine benthos in the intertidal zone.

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NF1, Sp1 and HSF1 are synergistically involved in sulfide-induced sqr activation in echiuran worm Urechis unicinctus

Cited by 9 publications

References 23 publications

DR4 mediates the progression, invasion, metastasis and survival of colorectal cancer through the Sp1/NF1 switch axis on genomic locus

DR4 mediates the progression, invasion, metastasis and survival of colorectal cancer through the Sp1/NF1 switch axis on genomic locus

Radial Expansion Favors the Burrowing Behavior of Urechis unicinctus

Genome-Wide Analyses of Heat Shock Protein Superfamily Provide New Insights on Adaptation to Sulfide-Rich Environments in Urechis unicinctus (Annelida, Echiura)

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