NF-Kβ Activation in U266 Cells on Mesenchymal Stem Cells

Zahedi, Sara; Shamsasenjan, Karim; Movassaghpour, Aliakbar; Akbarzadehlaleh, Parvin

doi:10.15171/apb.2016.054

Cited by 5 publications

(7 citation statements)

References 47 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, the effect of hBM‐MSCs on the proliferation of U937 cells was tested. For this purpose, hBM‐MSCs (1 × 10 4 cell/mL, confluency 80%) were co‐cultured in tissue culture flasks with U937 (1 × 10 5 cell/mL) cells for 48 hours . After the incubation, U937 cells were harvested and MTT assay was performed.…”

Section: Resultsmentioning

confidence: 99%

“…For this purpose, hBM-MSCs (1 × 10 4 cell/mL, confluency 80%) were co-cultured in tissue culture flasks with U937 (1 × 10 5 cell/mL) cells for 48 hours. 29 After the incubation, U937 cells were harvested and MTT assay was performed. The MTT assay on U937 cells indicated that hBM-MSCs support U937 cells proliferation, but in high concentration of hBM-MSCs their effects could be suppressive because of probable excess accumulation of different protein molecules ( Figure 1).…”

Section: The Hbm-mscs Supported U937 Cells Proliferationmentioning

confidence: 99%

See 1 more Smart Citation

Bone marrow ‐ mesenchymal stem cells impact on the U937 cells in the presence of staphylococcal enterotoxin B (SEB)

Ejtehadifar

Halabian

Ghazavi

et al. 2018

Clin Exp Pharma Physio

View full text Add to dashboard Cite

The growing resistance against conventional chemotherapy in acute myeloid leukemia (AML) is a noticeable clinical concern. Therefore, many researchers are looking for novel substances to overcome drug resistance in cancer. Staphylococcal enterotoxin B (SEB) is a superantigen (SAg) and a promising compound which has lethal effects on malignant cells. In this unprecedented study, SEB was used against U937 cells in a co-culture system in the presence of human bone marrow-mesenchymal stem cells (hBM-MSCs). The effects of hBM-MSCs on the proliferation and survival of U937 cell line with SEB was assessed using MTT assay and AnnexinV/PI flowcytometry, respectively. Moreover, the expression of IL-6, IL-10, TGF-β, and inhibitor of nuclear factor kappa-B kinase (IKKb) was evaluated by real-time PCR technique. The same experiments were also carried out using hBM-MSCs-conditioned medium (hBM-MSCs-CM). The results showed that SEB reduced the proliferation and survival of U937 cell line, but hBM-MSCs or hBM-MSCs-CM suppressed the effects of SEB. Furthermore, real-timePCR demonstrated that SEB could decrease the expression of IL-6, IL-10, and TGF-β in hBM-MSCs (P < .05), while the production of IKKb was increased in comparison with the control group. These findings help us to have a broader understanding ofthe usage of SEB in the treatment of haematological malignancies, especially if it is targeted against hBM-MSCs to disrupt their supportive effects on malignant cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: The Hbm-mscs Supported U937 Cells Proliferationmentioning

confidence: 99%

Bone marrow ‐ mesenchymal stem cells impact on the U937 cells in the presence of staphylococcal enterotoxin B (SEB)

Ejtehadifar

Halabian

Ghazavi

et al. 2018

Clin Exp Pharma Physio

View full text Add to dashboard Cite

show abstract

“…Quantitative real-time PCR RNA was extracted using an RNA extraction kit (Invitrogen), and cDNA was prepared using a cDNA synthesis kit (Biotech Rabbit, Berlin, Germany). The primer sets used were obtained from previous reports (30)(31)(32)(33)(34)(35)(36) and are shown in Table 1. For PCR, Luna ® Universal qPCR Master Mix (New England BioLabs, Ipswich, Massachusetts) was used at a concentration of 1 mM for forward and reverse primers.…”

Section: Immuno Uorescence Stainingmentioning

confidence: 99%

Human liver organoids are susceptible to Plasmodium vivax infection

Nitaramorn,

Kobpornchai,

Tongkrajang

et al. 2024

Preprint

View full text Add to dashboard Cite

The eradication of Plasmodium vivax malaria iscomplicated due to the presence of hypnozoites, the hidden dormant form of the parasite that is present in the liver. Currently available drug regimens are effective at killing hypnozoites but cause side effects and are difficult to administer. Studies testing drugs for liver-stage malaria remain rare and mainly relyon the use of cancerous or immortalized hepatic cells and primary hepatocytes. Here, we examined the feasibility of using an emerging organoid platform to model liver-stage vivax malaria. Hepatic endoderm cells, endothelial progenitor cells and mesenchymal cells were generated from human induced pluripotent stem cells and self-assembled intoliver organoids. These liver organoids resemble mature hepatocytes in terms of albumin secretion, fat and glycogen storage and cytochrome activity. Following the invasion of P. vivaxsporozoites, the expression levels of Plasmodium 18S RNA transcripts were upregulated. Changes in the transcript levels of genes involved in cholesterol synthesis were also observed. Thus, stem cell-derived liver organoids are susceptible to infection with P. vivax sporozoites, paving the way for studies on the mechanism of hypnozoite formation and testing of possible hypnozoitocidal drugs.

show abstract

“…[52,53] The structures 6-MAPDB (19), 5-MAPDB (20), 6-APDB (21), and 5-APDB (22) all are entactogenic drugs, and the 5-APDB ( 22) is a highly selective serotonin-releasing agent (SSRA). [54,55] Furthermore, the dihydrobenzofuran derivatives (23)(24)(25)(26)(27)(28) have been discovered in various species of higher plants and the Asteraceae family, all with critical biological activities (Figure 3). [56][57][58][59][60][61][62] The 1,3-dihydroisobenzofuran nucleus (2) (Figure 1) can be seen in several natural products and synthetic molecules with a variety of biological functions.…”

Section: Introductionmentioning

confidence: 99%

“…It is also a kind of important medicine intermediate found in the past few years simultaneously, such as anti‐tumor agent anti‐tubercular activity. The activity against the formation of cataracts, [21] and specific targets such as aldose reductase, [22] α‐glucosidase, [23] 5‐LOX, [24] COX‐2, [24] NF‐kβ, [25] and the muscarinic M3 receptor (Aperidine, Darifenacin, and Hordatine A) [26–28] . It also has anti‐oxidants, [29] and insecticidal properties [30,31] .…”

Section: Introductionmentioning

confidence: 99%

Recent Advances Towards the Synthesis of Dihydrobenzofurans and Dihydroisobenzofurans

et al. 2022

View full text Add to dashboard Cite

The 2,3-dihydrobenzofuran heterocyclic systems are found in various biologically active natural and pharmaceutical products of biological relevance such as Phalarine, Rocaglamide, and Furaquinocin A. The interest in the study of 2,3-dihydrobenzofurans is constantly increasing because of their numerous biological properties such as anti-tumor, anticancer, anti-tubercular, and anti-malarial activities. Different methods have been established towards the synthesis of 2,3dihydrobenzofurans involved via intra-and inter-molecular reactions. Besides, the 1,3-dihydroisobenzofuran structure has been identified as a fundamental nucleus in many natural products, bioactive compounds, and functional molecules (e. g., Pestacin, Isopestacin, Citalopram, Xylarinol B, Matriisobenzofuran, Thunberginol F7-O-β-D-glucopyranoside). On the other hand, 1,3-disubstituted isobenzofurans are powerful structural motifs with a wide range of biological activities, like anti-inflammatory, anti-histaminic, and anti-HIV. In the last few years, a decent number of diligent synthetic approaches have been demonstrated toward synthesizing 1,3-disubstituted isobenzofurans. This review focuses on the effective synthetic routes developed to construct 2,3-dihydrobenzofurans and 1,3-dihydroisobenzofurans and their applications in recent years.

show abstract

NF-Kβ Activation in U266 Cells on Mesenchymal Stem Cells

Cited by 5 publications

References 47 publications

Bone marrow ‐ mesenchymal stem cells impact on the U937 cells in the presence of staphylococcal enterotoxin B (SEB)

Bone marrow ‐ mesenchymal stem cells impact on the U937 cells in the presence of staphylococcal enterotoxin B (SEB)

Human liver organoids are susceptible to Plasmodium vivax infection

Recent Advances Towards the Synthesis of Dihydrobenzofurans and Dihydroisobenzofurans

Contact Info

Product

Resources

About