Next generation crop improvement program: Progress and prospect in tea ( Camellia sinensis (L.) O. Kuntze)

Hazra, Anjan; Dasgupta, Nirjhar; Sengupta, Chandan; Das, Sauren

doi:10.1016/j.aasci.2018.02.002

Cited by 19 publications

(16 citation statements)

References 94 publications

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“…Genotypic variation is one of the key factors by which the plant can differentially respond to a particular environmental stimuli 11 . In tea, challenges remained viable to explore the genetic diversity underlying varying physiological response to environment 12 , 13 . This study aimed to explore natural variations in the ecophysiological traits within seasons and the corresponding multifaceted biochemical responses given by the gene pool of 22 tea cultivars.…”

Section: Introductionmentioning

confidence: 99%

Ecophysiological traits differentially modulate secondary metabolite accumulation and antioxidant properties of tea plant [Camellia sinensis (L.) O. Kuntze]

Hazra

Saha

Dasgupta³

et al. 2021

Sci Rep

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Owing to the diverse growing habitats, ecophysiology might have a regulatory impact on characteristic chemical components of tea plant. This study aimed to explore natural variations in the ecophysiological traits within seasons and the corresponding multifaceted biochemical responses given by the gene pool of 22 tea cultivars. Leaf temperature and intercellular carbon concentration (Ci), which varies as a function of transpiration and net photosynthesis respectively, have significant impact on the biochemical traits of the leaf. Occurrence of H2O2, in leaves, was associated to Ci that in turn influenced the lipid peroxidation. With the increment of Ci, total phenolics, epicatechin gallate (ECG), reducing power, and radical scavenging activity is lowered but total catechin and non-gallylated catechin derivatives (e.g. epicatechin or EC, epigallocatechin or EGC) are elevated. Leaf temperature is concomitantly associated (p ≤ 0.01) with phenolics, flavonoids, proanthocyanidin, tannin content, reducing power, iron chelation and free radical scavenging activities. Increased phenolic concentration in leaf cells, conceivably inhibit photosynthesis and moreover, gallic acid, thereafter conjugated to catechin derivatives. This study shed light on the fundamental information regarding ecophysiological impact on the quality determining biochemical characteristics of tea, which on further validation, might ascertain the genotype selection paradigm toward climate smart cultivation.

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Section: Introductionmentioning

confidence: 99%

Ecophysiological traits differentially modulate secondary metabolite accumulation and antioxidant properties of tea plant [Camellia sinensis (L.) O. Kuntze]

Hazra

Saha

Dasgupta³

et al. 2021

Sci Rep

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“…This increased awareness demand the attention of scientific community for breeding new cultivars with improved yield and quality according to the choice of end users. However, unlike most of other agricultural crops, heterozygous woody perennial nature of tea plant limit the conventional breeding and improvement program in tea (Hazra et al, 2018). The repetitive large genome (~3GB) and polygenic inheritance nature of tea quality traits also hindered in generation of superior tea cultivar.…”

Section: Introductionmentioning

confidence: 99%

EFFICACY OF SEVERAL QTL AND miRNA-SSR MARKERS TOWARDS HEALTH BENEFIT TRAITS IN AN ELITE DARJEELING TEA CULTIVAR

Hazra¹,

Saha²,

Kumar³

et al. 2020

J microb biotech food sci

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Antioxidant and free radical scavenging ability of tea polyphenols are considered as health benefit traits that mainly contributed by tea catechins. Therefore understanding of genetic and molecular basis of this secondary metabolite production is of prime interest to facilitate the crop improvement program in tea plant efficiently. Some QTLs for these quality traits have already been available. In present study, 10 QTL linked SSRs and 14 newly developed microRNA based SSRs have been characterized in two Darjeeling tea cultivars having contrast characters and their progeny clone TS569 for validating possible association with catechin contents and antioxidant activities. Individual catechins and radical scavenging activity have been estimated by high performance liquid chromatography and UV-Vis spectrophotometry. Some of the polymorphic loci are indicated to be co-varied with such biochemical physiognomies. Characterization of microRNA based SSRs polymorphism are being the first time report in tea and discussed as ideal choice for further genotypic studies in tea.

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“…Hence, the selection and utilization of vigor cultivars with desirable phenotypes are crucial toward the prospective crop improvement program in tea. Development of trait-associated molecular markers has been adopted for tea in the recent past (Hazra et al, 2018a;Mukhopadhyay et al, 2016). Due to several bottlenecks in the life cycle, conventional association mapping has been found difficult in tea (Hazra et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

“…Due to several bottlenecks in the life cycle, conventional association mapping has been found difficult in tea (Hazra et al, 2020a). Hence, simultaneous genotyping and phenotyping of contrasting core accessions and subsequent marker-trait association studies were suggested as a convenient choice to overcome the challenges (Hazra et al, 2019;Hazra et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide SNP detection in Darjeeling tea: unravelling genetic structure, functional impact and trait associations

Hazra

Kumar

Sengupta

et al. 2020

Preprint

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Genotyping by sequencing and identification of functionally relevant nucleotide variations in crop accessions are the key steps to unravel genetic control of desirable traits. In the scope of present work, elite cultivars of Darjeeling tea were undergone SNP genotyping by double-digest restriction site associated DNA sequencing method. This study reports a set of 54,206 high-quality SNP markers discovered from ∼10.4 GB sequence data, encompassing 15 chromosomes of reference tea genome. Genetic relatedness among the accessions conforms to the analyses of Bayesian clustering, UPGMA, and PCoA methods. High percent of heterozygous loci in the majority of the cultivars reflect their ‘hybrid’ ancestry as reported earlier. Genomic positions of the discovered SNPs and their putative effect on annotated genes designated a thoughtful understanding of their functional aspects in tea system biology. A group of 95 genes was identified to be affected by high impact variants, most of them are involved in signal transduction, biosynthesis of secondary metabolite, transcriptional and translational regulation. Genome-wide association analyses of 21 agronomic and biochemical phenotypes resulted in trait-linked polymorphic loci with strong confidence (p < 0.05 and 0.001). The selection of significant marker-trait associations with the Bonferroni correction threshold retained a set of 57 SNPs distributed across 14 chromosomes to be linked with eight phenotypic traits. High impact and trait-associated nucleotide polymorphisms perceived in this study can be exploited in worldwide germplasm of contrasting origin to depict their heritability and to unlock their potentiality in marker-assisted breeding.

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Next generation crop improvement program: Progress and prospect in tea ( Camellia sinensis (L.) O. Kuntze)

Cited by 19 publications

References 94 publications

Ecophysiological traits differentially modulate secondary metabolite accumulation and antioxidant properties of tea plant [Camellia sinensis (L.) O. Kuntze]

Ecophysiological traits differentially modulate secondary metabolite accumulation and antioxidant properties of tea plant [Camellia sinensis (L.) O. Kuntze]

EFFICACY OF SEVERAL QTL AND miRNA-SSR MARKERS TOWARDS HEALTH BENEFIT TRAITS IN AN ELITE DARJEELING TEA CULTIVAR

Genome-wide SNP detection in Darjeeling tea: unravelling genetic structure, functional impact and trait associations

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