2010
DOI: 10.1369/jhc.2010.956870
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Abstract: The transcription factor octamer-binding transforming factor 4 (Oct-4) is central to the gene regulatory network responsible for self-renewal, pluripotency, and lineage commitment in embryonic stem (ES) cells and induced pluripotent stem cells (PSCs). This study was undertaken to evaluate differential localization and expression of two major transcripts of Oct-4, viz. Oct-4A and Oct-4B, in adult human testis. A novel population of 5- to 10-μm PSCs with nuclear Oct-4A was identified by ISH and immunolocalizatio… Show more

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Cited by 102 publications
(121 citation statements)
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References 53 publications
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“…Similar to our results, this antibody showed nuclear and also weak cytoplasmic signals (Warthemann et al, 2012). The authors suggested that this antibody was not capable of distinction between OCT4A and OCT4B isoforms and could also detect an unrelated epitope to OCT4 (Bhartiya et al, 2010;Warthemann et al, 2012). OCT4A is a nuclear protein that is involved in stemness and pluripotency, while OCT4B isoform is mainly present in cytoplasm and it is related to stress response of the cells (Cauffman et al, 2005).…”
Section: Resultssupporting
confidence: 87%
“…Similar to our results, this antibody showed nuclear and also weak cytoplasmic signals (Warthemann et al, 2012). The authors suggested that this antibody was not capable of distinction between OCT4A and OCT4B isoforms and could also detect an unrelated epitope to OCT4 (Bhartiya et al, 2010;Warthemann et al, 2012). OCT4A is a nuclear protein that is involved in stemness and pluripotency, while OCT4B isoform is mainly present in cytoplasm and it is related to stress response of the cells (Cauffman et al, 2005).…”
Section: Resultssupporting
confidence: 87%
“…We have reported for the first time the presence of a distinct population of VSELs with nuclear OCT-4 in adult mouse [48] and human [47] testis, located towards the basement membrane of the seminiferous tubules. Besides, we also detected a progenitor stem cell population with cytoplasmic OCT-4, which was slightly bigger and had abundant cytoplasm.…”
Section: Vsels In Mammalian Testismentioning
confidence: 85%
“…The Umbilical Cord Blood (UCB)-purified VSELs have also been reported to differentiate into neural cells [44] and after co-culture over OP-9 stroma cells were specified into HSCs similar to murine BM-derived VSELs [45]. Apart from umbilical cord blood and bone marrow, VSELs have also been reported in Wharton's jelly and gonadal tissue [46][47][48][49][50][51]. Their presence amongst the MSCs in the Wharton's jelly is in agreement with observations made by other groups that MSCs contain a sub-population of more primitive stem cells [52] or even as postulated by Taichman and group [53] that VSELs are precursors of MSCs.…”
Section: Developmental Origin Of Vselsmentioning
confidence: 99%
“…Although the concept of stem cells in adult ovaries is still not well accepted -the conflict appears to be more technical (Bhartiya 2012, Bhartiya et al 2013a) and hopefully will be resolved in due course. In addition to the seminal contributions made by Jonathan Tilly's group in the field, recently equating ovarian stem cells (OSCs) to spermatogonial stem cells (SSCs) in testis , and their protocols to isolate OSCs , our group has reported an additional population of stem cells (VSELs) in both ovary and testis (Bhartiya et al 2010, 2013b, Parte et al 2011, in addition to the OSCs/OGSCs.…”
Section: R41mentioning
confidence: 99%
“…On comparing POF in women with azoospermia in men, it is indeed intriguing that similar to POF, genetic basis for azoospermia is also not yet deciphered and no significant association is reported with the FSHR gene (or any other) and male infertility. VSELs exist in human (Bhartiya et al 2010) and mouse (Anand et al 2014) testis as in the ovaries and nuclear OCT4 is a specific and sensitive marker for testicular cancers (Jones et al 2004). Studies are currently ongoing in our laboratory to study the effect of FSH on testicular stem cells before we implicate FSH-FSHR3-stem cell interaction in testicular biology and azoospermia as well.…”
Section: R44 D Bhartiya and J Singhmentioning
confidence: 99%