New Tests for Bile and Detection of Bile in Serum and Urine

Abstract

1. New tests proposed for the detection of bilirubin have been described. 2. Application of some of the proposed tests on pathologic and normal serum and urine have been demonstrated. 3. The diazochlorides of certain amines previously reported as failures to detect bilirubin have been shown to be successful and have been applied to bile, serum, and urine. 4. Comparative sensitivity of the proposed tests and the most generally accepted tests for bilirubin in test solutions, pathologic serum, and urine have been given. 5. Spot tests suitable for the detection of bilirubin in pathologic urine have been presented.

Hemoglobin−Superoxide DismutaseChemical Linkages That Create a Dual-Function Protein

“…4-Maleimidobenzoic Acid . 4-Aminobenzoic acid (2.0 g, 1.5 × 10 -2 mol) and maleic acid (1.7 g, 1.5 × 10 -2 mol) were dissolved in 30 mL of dioxane and refluxed for 18 h in the presence of phosphorus pentoxide (3.0 g, 2.2 × 10 -2 mmol) . The reaction mixture was then filtered while hot.…”

Clinical Chemistry

“…Methods for serum and tissue lipide fractionation were described by Delsal (166) Liver Function. The diazochlorides of certain amines were used successfully for the detection of bilirubin in bile, serum, and urine by Barakat et al (41). A high-frequency technique was used by Johansson et al (316) for continuous recording in the chromatographic analysis of bile acids.…”