New strategy to surface functionalization of polymeric nanoparticles: one-pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules

Abstract

The one pot synthesis, using the ligand-metal-chitosan-lecithin complex to functionalize the surface of the biodegradable nanocapsules, maintained the active site of the antibody fragment making the device interesting for applications in nanomedicine.

Protective effects of melatonin-loaded lipid-core nanocapsules on paraquat-induced cytotoxicity and genotoxicity in a pulmonary cell line

“…This means that each nanoparticle in the suspension can carry about 1200 molecules of Mel bringing a large load of Mel to the target. In addition, LNC per se showed antioxidant properties [46]; LNC decreased ascorbyl radical formation, reducing in vitro lipid peroxidation of liposomes.…”

Novel therapeutic mechanisms determine the effectiveness of lipid-core nanocapsules on melanoma models

“…[61][62][63] It is beyond question that involvement of reactive oxygen species (ROS) and RNS affects melanoma induction and development, but the complexity of actions has generated conflicting data. [64][65][66] Therefore, we will make efforts to characterize the mechanisms of LNC on ROS and RNS systems and to establish the real role of ROS/RNS balance and its involvement in the toxicity of LNC on melanoma cells.…”

“…Indeed, recent studies have shown the biological activity of drug-free nanoparticles. The first reported the antioxidant effects of LNC in a lipid peroxidation model, 63 and the other showed the action of drug-free chitosancoated poly(iso-butyl cyanoacrylate) nanoparticles against Trichomonas vaginalis. 75 Hence, results herein highlight that the molecular interactions between the constituents of LNC affect the supramolecular structure as well as its physicochemical characteristics.…”

Nanodrug administration routes

“…In the active targeting, the nanoparticle is selectively recognized by receptors on the surface of cells. Since ligand-receptor interactions can be highly selective, which would allow for a more precise targeting of a specific site in the body [76,77,90,[93][94][95][96]. The approaches using active vectorization with nanoparticles are a quite challenging, and are currently one of the main goals of several studies in nanotechnology.…”

“…In another study [94], a ligand metal-CS-lecithin complex was prepared as a new strategy to functionalize the surface of PCL nanoparticles. The results showed that the nanoparticulate complex was able to connect recombinant antibody fragments, known as anti-electronegative LDL single-chain fragment variable [scFv anti-LDL(-)].…”