New sources of resistance to Phytophthora capsici in Capsicum spp.

Mo, Hwang-Sung; Kim, Soyoung; Wai, Khin Pa Pa; Siddique, Muhammad Irfan; Yoo, Hee-Ju; Kim, Byungsoo

doi:10.1007/s13580-014-0016-7

Cited by 18 publications

(10 citation statements)

References 20 publications

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“…Manitta was used as a susceptible control to compare the resistance levels. The final disease severity was scored at 4 weeks of inoculation based on the disease scale 0–4, where 0 (resistant) = no visible symptoms were observed; 1 (resistant) = a dark lesion visible on the base of the stem, but surviving without wilting; 2 (moderately resistant) = wilting with a dark lesion at the base of the stem; 3 (susceptible) = wilting with a black lesion on the stem’s middle; and 4 (highly susceptible) = wilting and death of the entire plant as described by Mo et al (2014) ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Association Study of Resistance to Phytophthora capsici in the Pepper (Capsicum spp.) Collection

Haile

Geum

et al. 2022

Front. Plant Sci.

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One of the most serious pepper diseases is Phytophthora blight, which is caused by Phytophthora capsici. It is crucial to assess the resistance of pepper genetic resources to Phytophthora blight, understand the genetic resistances, and develop markers for selecting resistant pepper materials in breeding programs. In this study, the resistance of 342 pepper accessions to P. capsici was evaluated. The disease severity score method was used to evaluate the phenotypic responses of pepper accessions inoculated with the KCP7 isolate. A genome-wide association study (GWAS) was performed to identify single nucleotide polymorphisms (SNPs) linked to P. capsici (isolate KCP7) resistance. The pepper population was genotyped using the genotype-by-sequencing (GBS) method, and 45,481 SNPs were obtained. A GWAS analysis was performed using resistance evaluation data and SNP markers. Significantly associated SNPs for P. capsici resistance at 4 weeks after inoculation of the GWAS pepper population were selected. These SNPs for Phytophthora blight resistance were found on all chromosomes except Chr.05, Chr.09, and Chr.11. One of the SNPs found on Chr.02 was converted into a high-resolution melting (HRM) marker, and another marker (QTL5-1) from the previous study was applied to pepper accessions and breeding lines for validation and comparison. This SNP marker was selected because the resistance phenotype and the HRM marker genotype matched well. The selected SNP was named Chr02-1126 and was located at 112 Mb on Chr.02. The Chr02-1126 marker predicted P. capsici resistance with 78.5% accuracy, while the QTL5-1 marker predicted resistance with 80.2% accuracy. Along with the marker for major quantitative traits loci (QTLs) on Chr.05, this Chr02-1126 marker could be used to accurately predict Phytophthora blight resistance in pepper genetic resources. Therefore, this study will assist in the selection of resistant pepper plants in order to breed new phytophthora blight-resistant varieties.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Association Study of Resistance to Phytophthora capsici in the Pepper (Capsicum spp.) Collection

Haile

Geum

et al. 2022

Front. Plant Sci.

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“…Candole et al (2010) manzano, pasilla, cola de rata, and jalapeño peppers. Similarly, genotypes of C. annuum with resistance to P. capsici have been reported in the United States (in New York and Michigan), Laos, and South Korea (Dunn et al, 2014;Foster and Hausbeck 2010;Mo et al, 2014;Su-Jung et al, 2014). A common problem among resistant pepper genotypes is that they show less resistance when assayed under different environmental conditions and P. capsici isolates (Dunn and Smart 2015;Messaouda et al, 2015).…”

Section: Discussionmentioning

confidence: 90%

Tolerance to Virulence Phenotypes of Phytophthora capsici in Pasilla Pepper Cultivars

Reyes-Tena¹,

Rodríguez-Alvarado²,

Luna‐Ruíz³

et al. 2021

horts

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Phytophthora capsici is the most important limiting factor in the production of chile pepper in Mexico. This pathogen presents virulence phenotypes capable of infecting diverse cultivars of this crop. The search and development of resistance in chile pepper is an excellent alternative for the management of P. capsici. The objective of this work was to evaluate the response of four pasilla pepper cultivars to infection with five virulence phenotypes of P. capsici. Pasilla pepper landraces PAS-1, PAS-2, PAS-3, and PAS-4 were inoculated with P. capsici isolates MX-1, MX-2, MX-7, MX-8, and MX-10. Two experiments were conducted under greenhouse conditions from April through June 2017 and April through June 2018. ‘California Wonder’ was included as a susceptible control, and uninoculated plants were included as a negative control. In each experiment, groups of six 56-day-old plants from each pepper cultivar were inoculated with each virulence phenotype. Disease severity was evaluated 20 days after inoculation using an individual plant severity scale. All pepper cultivars were classified as resistant = R, moderately resistant (MR), tolerant (T), moderately tolerant (MT), or susceptible (S), according to the frequency of resistant plants (severity 0–1). ‘California Wonder’ and ‘PAS-4’ were susceptible to all five virulence phenotypes. The rest had different responses to the virulence phenotypes, but ‘PAS-2’ and ‘PAS-3’ were susceptible to only one of the five virulence phenotypes. Pasilla peppers with low severity exhibited a slow rate of infection, which is a mechanism we have called “slow wilting.” The pasilla pepper cultivars PAS-1, PAS-2, and PAS-3 could be used in plant breeding programs as sources of genetic tolerance and moderate resistance against P. capsici.

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“…Based on the plant symptoms severity and the level of resistance when compared with the susceptible cultivar 'Bravo' and the resistant accession 'CM334' considered to have the highest known level of resistance (Candole et al, 2010;Mo et al, 2014), three resistance groups were identified. These results indicate that pepper landraces populations from Mexico could potentially have different genetic background and profiles that could be used in breeding programs.…”

Section: Discussionmentioning

confidence: 99%

Novel Sources of Resistance to Phytophthora capsici on Pepper (Capsicum sp.) Landraces from Mexico

et al. 2020

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Phytophthora capsici Leonian is a major pathogen of pepper worldwide and few resistance sources to this pathogen have been identified so far. The goals of this study were to identify new sources of resistance against P. capsici in Capsicum landraces and analyze the relationship between the resistance indicator of plant symptoms and some plant phenotype parameters of plant height, stem width, leaf length and leaf width. Thirtytwo landraces of pepper were collected from fourteen states in Mexico. From each population, 36 plants were inoculated with 10,000 zoospores of P. capsici under controlled conditions. This experiment was repeated twice. Out of the 32 landraces, six showed high level of resistance, four showed intermediate resistance and five showed low level of resistance when compared with the susceptible control 'Bravo' and the resistant control 'CM334', indicating that these landraces are promising novel sources of resistance to P. capsici. There was no correlation between the symptoms and plant phenotype parameters. However, these parameters were not affected in the group classified as highly resistant, indicating that P. capsici does not affect the growing of these resistant pepper landraces. The other resistant groups were significantly affected in a differently manner regarding their phenotype, indicating that this pathogen reduce their growth in different ways. This study reports novel resistance sources with great potential that could be used in breeding programs to develop new pepper cultivars with durable resistance to P. capsici.

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New sources of resistance to Phytophthora capsici in Capsicum spp.

Cited by 18 publications

References 20 publications

Genome-Wide Association Study of Resistance to Phytophthora capsici in the Pepper (Capsicum spp.) Collection

Genome-Wide Association Study of Resistance to Phytophthora capsici in the Pepper (Capsicum spp.) Collection

Tolerance to Virulence Phenotypes of Phytophthora capsici in Pasilla Pepper Cultivars

Novel Sources of Resistance to Phytophthora capsici on Pepper (Capsicum sp.) Landraces from Mexico

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