New serum biomarkers for detection of tuberculosis using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

Liu, Jiyan; Jin, Lei; Zhao, Mengyuan; Zhang, Xin; Liu, Chibo; Zhang, Yuxiang; Li, Fu-Jian; Zhou, Jian‐Min; Wang, Huajun; Li, Jicheng

doi:10.1515/cclm.2011.634

Cited by 13 publications

(9 citation statements)

References 33 publications

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“…However, the low detection rate of sputum smear‐positive and time‐consuming of sputum culture have restricted the diagnosis of pulmonary TB and discouraged early treatment of the disease. Research in our laboratory on TB biomarkers has demonstrated different peaks between TB and other lung diseases (2024, 8007, and 8598 Da) previously , but further studies are needed to identify these mass peaks. We also found that APOCII, CD5L, HABP2, and RBP4 may be potential pulmonary TB biomarkers, however, the experiment did not set the group for differential diagnosis.…”

Section: Discussionmentioning

confidence: 95%

Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ‐coupled two‐dimensional LC‐MS/MS

Li²,

et al. 2014

Proteomics

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This study aimed to discover the novel noninvasive biomarkers for the diagnosis of pulmonary tuberculosis (TB). We applied iTRAQ 2D LC-MS/MS technique to investigate protein profiles in patients with pulmonary TB and other lung diseases. A total of 34 differentially expressed proteins (24 upregulated proteins and ten downregulated proteins) were identified in the serum of pulmonary TB patients. Significant differences in protein S100-A9 (S100A9), extracellular superoxide dismutase [Cu-Zn] (SOD3), and matrix metalloproteinase 9 (MMP9) were found between pulmonary TB and other lung diseases by ELISA. Correlations analysis revealed that the serum concentration of MMP9 in the pulmonary TB was in moderate correlation with SOD3 (r = 0.581) and S100A9 (r = 0.471), while SOD3 was in weak correlation with S100A9 (r = 0.287). The combination of serum S100A9, SOD3, and MMP9 levels could achieve 92.5% sensitivity and 95% specificity to discriminate between pulmonary TB and healthy controls, 90% sensitivity and 87.5% specificity to discriminate between pulmonary TB and pneumonia, and 85% sensitivity and 92.5% specificity to discriminate between pulmonary TB and lung cancer, respectively. The results showed that S100A9, SOD3, and MMP9 may be potential diagnostic biomarkers for pulmonary TB, and provided experimental basis for the diagnosis of pulmonary TB.

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Section: Discussionmentioning

confidence: 95%

Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ‐coupled two‐dimensional LC‐MS/MS

Li²,

et al. 2014

Proteomics

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“…But the authors did not establish the differential diagnosis groups, and verified two serum markers in a large sample. In our laboratory, Liu et al [18] detected specific protein markers for the diagnosis of TB, and established a model based on the three biomarkers (2024, 8007, 8598 Da) to differentiate between patients with TB and other lung diseases by using surface enhanced laser desorption/ionization - time of flight - mass spectrometry (SELDI-TOF-MS). However, due to poor reproducibility of results, further study is needed for screening pulmonary TB biomarkers.…”

Section: Discussionmentioning

confidence: 99%

Screening and Identification of Six Serum microRNAs as Novel Potential Combination Biomarkers for Pulmonary Tuberculosis Diagnosis

et al. 2013

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BackgroundIt is very difficult to prevent pulmonary tuberculosis (TB) due to the lack of specific and diagnostic markers, which could lead to a high incidence of pulmonary TB. We screened the differentially expressed serum microRNAs (miRNAs) as potential biomarkers for the diagnosis of pulmonary TB.MethodsIn this study, serum miRNAs were screened using the Solexa sequencing method as the potential biomarkers for the diagnosis of pulmonary TB. The stem-loop quantitative reverse-transcription polymerase chain reaction (qRT-PCR) assay was used to verify differentially expressed serum miRNAs. The receiver operating characteristic (ROC) curve and logistic regression model were used to analyze the sensitivity and specificity of the single miRNA and a combination of miRNAs for diagnosis, respectively. Using the predicted target genes, we constructed the regulatory networks of miRNAs and genes that were related to pulmonary TB.ResultsThe Solexa sequencing data showed that 91 serum miRNAs were differentially expressed in pulmonary TB patients, compared to healthy controls. Following qRT-PCR confirmation, six serum miRNAs (hsa-miR-378, hsa-miR-483-5p, hsa-miR-22, hsa-miR-29c, hsa-miR-101 and hsa-miR-320b) showed significant difference among pulmonary TB patients, healthy controls (P<0.001) and differential diagnosis groups (including patients with pneumonia, lung cancer and chronic obstructive pulmonary disease) (P<0.05). The logistic regression analysis of a combination of six serum miRNAs revealed that the sensitivity and the specificity of TB diagnosis were 95.0% and 91.8% respectively. The miRNAs-gene regulatory networks revealed that several miRNAs may regulate some target genes involved in immune pathways and participate in the pathogenesis of pulmonary TB.ConclusionOur study suggests that a combination of six serum miRNAs have great potential to serve as non-invasive biomarkers of pulmonary TB.

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“…Delayed diagnosis results in more extensive disease, complications, and increased mortality. 16 Spinal TB usually presents in a slowly indolent manner with nonspecific clinical presentations, making the diagnosis a great challenge for clinicians. A definite diagnosis of spinal TB depends on the demonstration of M. tuberculosis by culture from biopsy specimens.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-linked immunospot assay response to recombinant CFP-10/ESAT-6 fusion protein among patients with spinal tuberculosis: implications for diagnosis and monitoring of surgical therapy

Yuan

Zhang³

et al. 2013

International Journal of Infectious Diseases

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New serum biomarkers for detection of tuberculosis using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

Abstract: The data suggested a potential application of SELDI-TOF MS as an effective technology to profile serum proteome, and with pattern analysis, a diagnostic model comprising three potential biomarkers was indicated to differentiate people with TB and healthy controls rapidly and precisely.

Cited by 13 publications

References 33 publications

Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ‐coupled two‐dimensional LC‐MS/MS

Serum protein S100A9, SOD3, and MMP9 as new diagnostic biomarkers for pulmonary tuberculosis by iTRAQ‐coupled two‐dimensional LC‐MS/MS

Screening and Identification of Six Serum microRNAs as Novel Potential Combination Biomarkers for Pulmonary Tuberculosis Diagnosis

Enzyme-linked immunospot assay response to recombinant CFP-10/ESAT-6 fusion protein among patients with spinal tuberculosis: implications for diagnosis and monitoring of surgical therapy

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