New processes and actual trends in biotechnology

Gerbsch, N.; Buchholz, Rainer

doi:10.1111/j.1574-6976.1995.tb00173.x

Cited by 36 publications

(4 citation statements)

References 26 publications

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“…Incorporation of microorganisms in biofilms is a natural way for their immobilization. The immobilization of cells in polysaccharide gel matrices, particularly Ca-alginate hydrogels, is the most frequently used method for cell entrapment [212,213]. The high cell density of biofilms, both natural and artificial, gives them substantial biocatalytic potential.…”

Section: Microbial Biofilmsmentioning

confidence: 99%

Application of proteomics in biotechnology – Microbial proteomics

Josić

Kovač

2008

Biotechnology Journal

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The genomes of most economically important microbial cells are already sequenced and proteomic technologies can be applied during various process development steps, starting with the selection and optimization of the functions of the industrial strains, application of the knowledge of cell function in response to the changes of production parameters, validation of the downstream processing, and thorough characterization of the final product. Unfortunately, there are only a few direct examples in the literature that present the optimization of the production process based on proteomics. In this review, we discuss the potential of this technology for the design of future bioprocesses and for optimization of existing ones.

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Section: Microbial Biofilmsmentioning

confidence: 99%

Application of proteomics in biotechnology – Microbial proteomics

Josić

Kovač

2008

Biotechnology Journal

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“…Highly productive devices have enabled the preparation of beads with a controlled, narrow distribution of size and shape from a laboratory scale to an industrial scale [1]. Water-soluble polysaccharides were the most frequently used materials for entrapment due to the simple and nontoxic formation of hydrogel beads by ionotropic gelation using divalent cations such as Ca 2+ [2]. However, the development of thermoreversible gelification of polyvinylalcohol (PVA) in the form of lens-shaped particles, known as LentiKats® [3] allowed many applications due to the higher catalytic efficiency, mechanical stability, as well as the lower mass transfer resistance and cost of these particles with entrapped cells or enzymes [4,5].…”

Section: Introductionmentioning

confidence: 99%

Physical and Bioengineering Properties of Polyvinyl Alcohol Lens-Shaped Particles Versus Spherical Polyelectrolyte Complex Microcapsules as Immobilisation Matrices for a Whole-Cell Baeyer–Villiger Monooxygenase

Schenkmayerová

Bučko

Gemeiner

et al. 2014

Appl Biochem Biotechnol

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Direct comparison of key physical and chemical-engineering properties of two representative matrices for multipurpose immobilisations was performed for the first time. Polyvinyl alcohol lens-shaped particles LentiKats® and polyelectrolyte complex microcapsules were characterised by advanced techniques with respect to the size distribution of the particles, their inner morphology as revealed by fluorescent probe staining, mechanical resistance, size-exclusion properties, determination of effective diffusion coefficient and environmental scanning electron microscope imaging. While spherical polyelectrolyte complex microcapsules composed of a rigid semipermeable membrane and a liquid core are almost uniform in shape and size (diameter of 0.82 mm; RSD = 5.6 %), lens-shaped LentiKats® are characterised by wider size distribution (diameter of 3.65 mm; RSD = 10.3 % and height of 0.341 mm; RSD = 32.3 %) and showed the same porous structure throughout their whole volume at the mesoscopic (micrometre) level. Despite differences in their inner structure and surface properties, the pore diameter of ∼ 2.75 nm for regular polyelectrolyte complex microcapsules and ∼ 1.89 nm for LentiKats® were similar. These results were used for mathematical modelling, which provided the estimates of the effective diffusion coefficient of sucrose. This value was 1.67 × 10(-10) m(2) s(-1) for polyelectrolyte complex microcapsules and 0.36 × 10(-10) m(2) s(-1) for LentiKats®. Recombinant cells Escherichia coli-overexpressing enzyme cyclopentanone monooxygenase were immobilised in polyelectrolyte complex microcapsules and LentiKats® for comparison of their operational stability using model Baeyer-Villiger oxidation of (±)-cis-bicyclo [3.2.0] hept-2-en-6-one to regioisomeric lactones as important chiral synthons for potential pharmaceuticals. Both immobilisation matrices rendered high operational stability for whole-cell biocatalyst with no reduction in the biooxidation rate over 18 repeated reaction cycles.

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“…Immobilization of microorganisms in general has been of interest since approximately 1800, and industrial exploitation has been reported since 1964 [ 20 ]. In principle, the methods can be applied to microalgae, taking into account the requirement for light of the photosynthetically active cells and their sensitivity [ 12 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Silica Hydrogels as Entrapment Material for Microalgae

Homburg

Patel

2022

Polymers

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Despite being a promising feedstock for food, feed, chemicals, and biofuels, microalgal production processes are still uneconomical due to slow growth rates, costly media, problematic downstreaming processes, and rather low cell densities. Immobilization via entrapment constitutes a promising tool to overcome these drawbacks of microalgal production and enables continuous processes with protection against shear forces and contaminations. In contrast to biopolymer gels, inorganic silica hydrogels are highly transparent and chemically, mechanically, thermally, and biologically stable. Since the first report on entrapment of living cells in silica hydrogels in 1989, efforts were made to increase the biocompatibility by omitting organic solvents during hydrolysis, removing toxic by-products, and replacing detrimental mineral acids or bases for pH adjustment. Furthermore, methods were developed to decrease the stiffness in order to enable proliferation of entrapped cells. This review aims to provide an overview of studied entrapment methods in silica hydrogels, specifically for rather sensitive microalgae.

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New processes and actual trends in biotechnology

Cited by 36 publications

References 26 publications

Application of proteomics in biotechnology – Microbial proteomics

Application of proteomics in biotechnology – Microbial proteomics

Physical and Bioengineering Properties of Polyvinyl Alcohol Lens-Shaped Particles Versus Spherical Polyelectrolyte Complex Microcapsules as Immobilisation Matrices for a Whole-Cell Baeyer–Villiger Monooxygenase

Silica Hydrogels as Entrapment Material for Microalgae

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