New phytochemical constituent and bioactivities ofHorwoodia dicksoniaeandRumex cyprius

Abstract

Background:Many plants growing in Saudi Arabia are used in folk medicine for treatment of several diseases.Objective:Information of the chemical constituents and biological activities of plants is desirable for the discovery of therapeutic agents and discovering the actual value of folkloric remedies.Materials and Methods:The compounds were isolated and purified using silica gel column chromatography and preparative high-performance liquid chromatography-diode array detector (HPLC-DAD) Method. The alcoholic extracts of these plants were evaluated for biological activities.Results:Isolation and characterization of 1-feruloyl-β-D-glucopyranoside (1) as well as new secondary metabolite tryptophan methyl ester (2) were isolated for the 1st time from the Horwoodia dicksoniae. The three flavones were isolated from Rumex cyprius identified as isoorientin (3), vitexin (4), and Cynarosid (5). The structures of these compounds were characterized by nuclear magnetic resonance and mass spectrometry analysis and comparing with literature. The compounds were isolated and purified using silica gel column chromatography and preparative HPLC-DAD Method. The alcoholic extracts of these plants were evaluated for antimicrobial activities against two Gram-positive bacteria, two Gram-negative bacteria, and four pathogenic fungi. Both plants showed good activities against Syncephalastrum racemosum and Streptococcus pneumoniae with minimal inhibitory concentrations (MICs) 0.98 and 1.95 μg/mL, respectively. H. dicksoniae showed good activity against Aspergillus fumigates with an MIC 1.95 μg/mL. The two extracts showed also effective free radical scavenging activities in the 1,1-diphenyl-2-picrylhydrazyl assay. H. dicksoniae exhibited remarkable cytotoxic activity against Human breast cancer mammary cancer cells-7, Human liver cancer human hepatoma carcinoma cells-2, and human lung carcinoma (A-549) cell lines.Conclusions:It was suggested that further work should be carried out to isolate, purify, and characterize the active constituents responsible for the activity of these plants.SUMMARY New secondary metabolite Tryptophane methyl ester as well as 1-feruloyl-β-D-glucopyranoside were isolated for the first time from the HD.Isoorientin, vitexin and Cynarosid were isolated from RC.HD exhibited good activity against Aspergillus fumigates with an MIC 1.95 µg mL-1.HD showed significant cytotoxic activity against Human breast cancer (MCF-7), Human liver cancer (HepG-2) and Human lung carcinoma (A-549) cell lines.

Phenolic Compounds of Rumex roseus L. Extracts and Their Effect as Antioxidant and Cytotoxic Activities

“…Also, Wang et al [33] found that with the significant antioxidant activities exhibited by shikonin, it has shown also a powerful cytotoxic activity with IC 50 equal to 0.83 and 0.53 (μg/mL), respectively, for the two cell lines MCF-7 and HCT-116. Furthermore, these results of R. roseus extracts were higher than those found by other studies done on plants of the same family (Polygonaceae); they showed that the extract from the species Rumex crispus, for example, had no cytotoxic activity (0%) against the MCF-7 cell line [34] and in others as well (Polygonum equisetiforme); a low activity against both MCF-7 and HCT-116 was found, with 11.3 and 14.5%, respectively [35].…”

Phytochemical Composition, Antioxidant, Antiacetylcholinesterase, and Cytotoxic Activities of Rumex crispus L.

“…In addition, for both cell lines, HCT-116 and MCF-7, the DCM extract revealed the highest cytotoxic potential with almost 69.2 and 77.2% of inhibition, respectively. This inhibition may be due to the presence of some phenolic compounds, which are known for their important cytotoxic activities, especially cardamonin with IC 50 equal to 8.1 and 8.6 µ g/mL, respectively, against the two cell lines (MCF-7 and HCT-116) [ 65 ]. Other studies suggested potent activity against cancer cells, including MCF-7 and HCT-116 cell lines, among which shikonin molecule (IC 50 = 0.83 and 0.53 ( μ g/ml)), respectively [ 66 ], which confirms our obtained results.…”

“…In addition, for both cell lines, HCT-116 and MCF-7, the DCM extract revealed the highest cytotoxic potential with almost 69.2 and 77.2% of inhibition, respectively. is inhibition may be due to the presence of some phenolic compounds, which are known for their important cytotoxic activities, especially cardamonin with IC 50 equal to 8.1 and 8.6 µg/mL, respectively, against the two cell lines (MCF-7 and HCT-116) [65].…”

Antioxidant and Anti-inflammatory Activities of Plants Extracts of Israel and Palestine. Unexplored Paradise

“…110 New studies of non-local species reveal the health promoting phytochemicals that they contain. 111 The structures of these compounds are shown in Figure 6.…”