New oleanane saponins from the roots of <i>Dendrobangia boliviana</i> identified by LC‐SPE‐NMR

Zebiri, Ilhem; Gratia, Audrey; Haddad, Mohamed; Cabanillas, Billy; Harakat, Dominique; Voutquenne-Nazabadioko, Laurence

doi:10.1002/mrc.4619

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Peak 16 showed an [M+HCOO] − ion at m/z 723 and produced fragment ion at m/z 677 [M–H] − and m/z 485 in negative MS 2 mode. Compared with standard and the literature ( 30 ), peak 16 was deduced as zebirioside O. The [M-162-46-H] − ion in the MS 2 spectrum of peaks 17, 20, 21, 22, 23, and 24 corresponding to the presence of glucose group in the compounds.…”

Section: Resultsmentioning

confidence: 90%

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

et al. 2022

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The microfluidic platform is a versatile tool for screening and locating bioactive molecules from functional foods. Here, a layer-by-layer assembly approach was used to fabricate core-shell CdSSe@ZnS quantum dot encoded superparamagnetic iron oxide microspheres, which served as a carrier for matrix metalloproteinase-2. The matrix metalloproteinase-2 camouflaged magnetic microspheres was further incorporated into a homemade microfluidic platform and incubated with extracts of fruits of Rosa roxburghii. The flow rate of the microfluidic platform was tuned. The major influencing parameters on ligand binding, such as dissociate solvents, incubation pH, ion strength, temperature, and incubation time were also optimized by using ellagic acid as a model compound. The specific binding ligands were sent for structure elucidation by mass spectrometry. The absolute recovery of ellagic acid ranged from 101.14 to 102.40% in the extract of R. roxburghii under the optimal extraction conditions. The linearity was pretty well in the range of 0.009–1.00 mg·ml−1 (R2 = 0.9995). The limit of detection was 0.003 mg·ml−1. The relative SDs of within-day and between-day precision were <1.91%. A total of thirteen ligands were screened out from fruits of R. roxburghii, which were validated for their inhibitory effect by enzyme assay. Of note, eleven new matrix metalloproteinase-2 inhibitors were identified, which may account for the antitumor effect of fruits of R. roxburghii.

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Section: Resultsmentioning

confidence: 90%

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

et al. 2022

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“…HPLC-SPE-NMR orientated research is always oriented towards the structural characterization of analytes. Major metabolite classes addressed in recent years were, for example, alkaloids [ 117 , 118 , 119 , 120 , 121 ], diarylheptanoids [ 122 ], flavonoids [ 123 , 124 ], isoflavonoids [ 125 ], chromenes [ 126 ], lignans [ 127 , 128 ], phenanthrenes [ 129 ], terpenoids of different sub-classes [ 130 , 131 , 132 ], steroids [ 133 , 134 ], iridoids [ 135 ], polyphenols [ 136 ], and saponins [ 137 , 138 ].…”

Section: Applications Examplesmentioning

confidence: 99%

NMR-Based Chromatography Readouts: Indispensable Tools to “Translate” Analytical Features into Molecular Structures

Seger

Sturm

2022

Cells

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Gaining structural information is a must to allow the unequivocal structural characterization of analytes from natural sources. In liquid state, NMR spectroscopy is almost the only possible alternative to HPLC-MS and hyphenating the effluent of an analyte separation device to the probe head of an NMR spectrometer has therefore been pursued for more than three decades. The purpose of this review article was to demonstrate that, while it is possible to use mass spectrometry and similar methods to differentiate, group, and often assign the differentiating variables to entities that can be recognized as single molecules, the structural characterization of these putative biomarkers usually requires the use of NMR spectroscopy.

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Dereplication and Identification of Natural Products Using LC-NMR Based Strategies

Brkljača¹,

Wolfender²,

Urban³

2020

Comprehensive Natural Products III

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New oleanane saponins from the roots of Dendrobangia boliviana identified by LC‐SPE‐NMR

Cited by 3 publications

References 10 publications

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

NMR-Based Chromatography Readouts: Indispensable Tools to “Translate” Analytical Features into Molecular Structures

Dereplication and Identification of Natural Products Using LC-NMR Based Strategies

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