New Loci Including Pse‐6 Conferring Resistance to Halo Bacterial Blight on Chromosome Pv04 in Common Bean

Abstract

Halo bacterial blight caused by Pseudomonas syringae pv. phaseolicola (Psp) is a serious seed‐borne disease of common bean (Phaseolus vulgaris L.). The disease can be controlled by major resistance (R) genes effective against specific races of the pathogen. Our objective was to characterize the R gene(s) conferring resistance to Psp Races 1, 5, 7, and 9 in the great northern dry bean germplasm release BelNeb‐RR‐1. A recombinant inbred population consisting of 76 F9–derived lines (RILs) from the cross BelNeb‐RR‐1/A55 was inoculated with Races 1, 5, 7, and 9 and genotyped with 5398 single nucleotide polymorphism markers. Resistance to Races 1, 5, 7, and 9 cosegregated with each other and mapped to the proximal end of Pv04 in a known region of R genes that condition resistance to bean anthracnose and bean rust. Location on Pv04, combined with F2 allelism tests, confirmed the presence of a new gene, Pse‐6, for halo blight resistance. We mapped other unnamed R genes to proximal end of Pv04 that were segregating in Minuette/OSU5630 and Raven/I9365‐31 RIL populations and that conditioned resistance to Psp Race 7 and Race 1, respectively. A second R gene, Pse‐4, conditioning resistance solely to Race 5, was detected in BelNeb‐RR‐1 and tentatively positioned on chromosome Pv10. A sequence‐characterized amplified region marker SB10.550 tightly linked (1.4 cM) with Pse‐6 was generated for potential use in marker‐assisted selection for resistance to halo bacterial blight.

Genome-Wide Linkage and Association Mapping of Halo Blight Resistance in Common Bean to Race 6 of the Globally Important Bacterial Pathogen

“…The severe hypersensitive reaction to Race 3 (conferred by Pse-3 ) co-segregated with the I gene locus on Pv02 (48.398–48.534 Mb) (Figure 2C, right graph; Table S1), which is consistent with previous observations (Teverson, 1991; Miklas et al, 2011, 2014). Linked markers show a pattern of segregation distortion in favor of the parent possessing the resistance allele similar to that observed in the RC population (described below).…”

“…From the combined data, they proposed a gene-for-gene model involving five major-effect, race-specific plant resistance ( R ) genes and corresponding pathogen avirulence ( avr ) genes (Taylor et al, 1996b). Subsequent genetic research based on linkage mapping identified the locations of major-effect R genes ( Pse-1 to Pse-6 ) on three chromosomes of P. vulgaris (Pv02, Pv04, and Pv10), which are effective against one or more Psph races (Miklas et al, 2009, 2011, 2014). …”

Genetic Characterization of Green Bean (Phaseolus vulgaris L.) Accessions from Turkey with SCAR and SSR Markers

“…Among reference cultivars, only MDRK carried 520 bp allele, while the others carrying\500 bp band. Recently, Miklas et al (2014) reported SB10.550 originating from the previously mapped RAPD marker B10.525 tightly linked (1.4 cM) with Pse-6 as a potential marker for resistance to halo bacterial blight.…”

“…Genetic linkage maps in common bean provided the positioning of QTLs and identified markers tightly linked to the target loci (Blair et al 2003;Miklas et al 2014;Keller et al 2015). Reliable markers for resistance against biotic and abiotic stress factors provided successful application of MAS (marker-assisted selection) in common bean (Fourie et al 2004;Kelly and Vallejo 2004;Miklas et al 2014). …”

Detection of Ten Resistance Genes Against P. syringae pv. phaseolicola and X. axonopodis pv. phaseoli in Twelve Local Bean Varieties Using SCAR Markers

“…phaseolicola and six resistance genes (SAP6, BAC6, SU91, BC420, R7313 and R4864) for X. axonopodis pv. phaseoli (Carlos et al, 2009;Perry et al, 2013;Miklas et al, 2014).…”