New generation of nitric oxide-releasing porous materials: Assessment of their potential to regulate biological functions

Pinto, R.; Fernándes, Ana C.; Antunes, Fernando; Lin, Zhi; Rocha, João; Pires, João; Pinto, Moisés L.

doi:10.1016/j.niox.2019.05.010

Cited by 16 publications

(13 citation statements)

References 43 publications

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“…Current therapeutic NO applications are limited by pharmacodynamics, pharmacokinetics, or systemic absorption and toxicity [207,326]. To help overcome these challenges, a number of NO-releasing and -containing compounds have been developed that enhance the pharmacodynamic properties of NO as an anticancer agent.…”

Section: Future Directionsmentioning

confidence: 99%

“…Further modification of the NO-drug-releasing compounds to nanoparticles and liposomes has been shown to increase the tumor selectivity, half-life, or wavelength responsiveness of NO-donating drugs combined with macromolecules [207,334]. Other organic and inorganic polymers or porous materials have also been proposed as NO-releasing agents, although their efficacy and biocompatibility are still under study [207,326]. Although preclinical studies of NO-donating macromolecules are promising, few studies have tested the efficacy of these compounds in vivo, suggesting that clinical applications of such compounds are far off.…”

Section: Future Directionsmentioning

confidence: 99%

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Current Advances of Nitric Oxide in Cancer and Anticancer Therapeutics

et al. 2021

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Nitric oxide (NO) is a short-lived, ubiquitous signaling molecule that affects numerous critical functions in the body. There are markedly conflicting findings in the literature regarding the bimodal effects of NO in carcinogenesis and tumor progression, which has important consequences for treatment. Several preclinical and clinical studies have suggested that both pro- and antitumorigenic effects of NO depend on multiple aspects, including, but not limited to, tissue of generation, the level of production, the oxidative/reductive (redox) environment in which this radical is generated, the presence or absence of NO transduction elements, and the tumor microenvironment. Generally, there are four major categories of NO-based anticancer therapies: NO donors, phosphodiesterase inhibitors (PDE-i), soluble guanylyl cyclase (sGC) activators, and immunomodulators. Of these, NO donors are well studied, well characterized, and also the most promising. In this study, we review the current knowledge in this area, with an emphasis placed on the role of NO as an anticancer therapy and dysregulated molecular interactions during the evolution of cancer, highlighting the strategies that may aid in the targeting of cancer.

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Section: Future Directionsmentioning

confidence: 99%

Section: Future Directionsmentioning

confidence: 99%

Current Advances of Nitric Oxide in Cancer and Anticancer Therapeutics

et al. 2021

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“…Zeolites, titanosilicates and metal-organic frameworks (MOFs) are examples of successful carriers capable of storing therapeutic NO amounts [3][4][5]. They release it in its pure form by exposing the material to aqueous biological environments [6].…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Different Approaches to Quantify Nitric Oxide Release from NO-Releasing Materials in Relevant Biological Media

et al. 2020

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The development of solid materials that deliver nitric oxide (NO) are of interest for several therapeutic applications. Nevertheless, due to NO’s reactive nature, rapid diffusion and short half-life, reporting their NO delivery characteristics is rather complex. The full knowledge of this parameter is fundamental to discuss the therapeutic utility of these materials, and thus, the NO quantification strategy must be carefully considered according to the NO-releasing scaffold type, to the expected NO-releasing amounts and to the medium of quantification. In this work, we explore and discuss three different ways of quantifying the release of NO in different biological fluids: haemoglobin assay, Griess assay and NO electrochemical detection. For these measurements, different porous materials, namely zeolites and titanosilicates were used as models for NO-releasing platforms. The oxyhaemoglobin assay offers great sensitivity (nanomolar levels), but it is only possible to monitor the NO release while oxyhaemoglobin is not fully converted. On the other hand, Griess assay has low sensitivity in complex biological media, namely in blood, and interferences with media make NO measurements questionable. Nevertheless, this method can measure micromolar amounts of NO and may be useful for an initial screening for long-term release performance. The electrochemical sensor enabled real-time measurements in a variety of biological settings. However, measured NO is critically low in oxygenated and complex media, giving transient signals, which makes long-term quantification impossible. Despite the disadvantages of each method, the combination of all the results provided a more comprehensive NO release profile for these materials, which will help to determine which formulations are most promising for specific therapeutic applications. This study highlights the importance of using appropriate NO quantification tools to provide accurate reports.

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“…Furthermore, the Al-and Gaexchanged materials exhibited a significant lower toxicity than the Cu-ETS-4 analogs and no metal leaching was observed when incubated in cell culture medium. 31 Although the properties of titanosilicates as NO-storing solids are promising, the assessment of their ability to regulate biological functions is essential toward their evolution to real therapeutic applications. With this aim in mind, Pinto and co-workers studied the biocompatibility, chemical stability and NO-release in biological medium of the above-mentioned materials.…”

Section: Coordination On Open Metal Sitesmentioning

confidence: 99%

“…NO-loaded ETS-4 proved to elicit biological responses due to NO-release by reversibly inhibiting mitochondrial oxygen consumption in HeLa cells and promote cell migration, highlighting its potential application in wound healing. 31…”

Section: Coordination On Open Metal Sitesmentioning

confidence: 99%