New findings of Neurospora in Europe and comparisons of diversity in temperate climates on continental scales

Jacobson, David J.; Dettman, Jeremy R.; Adams, Rachel I.; Boesl, Cornelia; Sultana, Shahana; Roenneberg, Till; Merrow, Martha; Duarte, Margarida; Marques, Isabel; Ushakova, Alexandra V.; Carneiro, Patrı́cia; Videira, Arnaldo; Navarro‐Sampedro, Laura; Olmedo, María; Corrochano, Luis M.; Taylor, John W.

doi:10.3852/mycologia.98.4.550

Cited by 57 publications

(38 citation statements)

References 19 publications

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“…Fungal spore killers were fi rst discovered in Neurospora among natural populations of N. sitophila (Sk-1) and N. intermedia (Sk-2 and Sk-3). Sk-2 and Sk-3 have been found in only four N. intermedia isolates from Borneo (Brunei, Sabah), Java and Papua New Guinea among ~2500 isolates of this species from around the world, whereas Sk-1 killer is found in up to 30% of N. sitophila isolates from many parts of the world (Turner and Perkins 1979;Turner 2001;Jacobson et al 2006).…”

Section: Spore Killers As Meiotic Drive Elementsmentioning

confidence: 96%

“…More recent collections in the temperate regions of North America and Europe following forest fi res, however, gave a different population structure: N. discreta, N. crassa and N. sitophila were more common than N. intermedia. Subsequent analyses of wild strains provided insights into the diversity and evolution of this model organism (Jacobson et al 2004(Jacobson et al , 2006. To this day, the wild isolates continue to serve as a valuable resource and studies with them have contributed to several major discoveries: vegetative incompatibility genes, transposable elements, senescence-causing mitochondrial plasmids, recessive sexual phase mutants and meiotic driveinducing spore killers, to name a few.…”

Section: Natural Populationsmentioning

confidence: 99%

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Neurospora as a model fungus for studies in cytogenetics and sexual biology at Stanford

Raju

2009

J Biosci

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Dodge's early work (1927-1940) on Neurospora genetics and sexual biology inspired Beadle and Tatum at Stanford to use N.crassa for their landmark discovery that genes specify enzymes. Neurospora has since become a model organism for numerous genetic, cytogenetic, biochemical, molecular and population biology studies. Neurospora is haploid in the vegetative phase with a transient diploid sexual phase. Its meiotic cells (asci) are large, allowing easy examination of dividing nuclei and chromosomes under a light microscope. The haploid meiotic products are themselves the sexual progeny that grow into vegetative cultures, thus avoiding the cumbersome testcrosses and complex dominance -recessive relationships, as in diploid organisms.The Perkins'laboratory at Stanford (1949-2007) played a pivotal role in advancing our knowledge of Neurospora genetics, sexual biology, cytogenetics and population biology. Since 1974, I have taken advantage of various chromosome-staining methods to examine ascus and ascospore development in wild type and in numerous mutant strains. In addition,I have used GFP-tagged genes to visualize the expression or silencing of unpaired genes in a post-transcriptional gene silencing process (meiotic silencing by unpaired DNA) that operates specifically during meiosis. The genome of N. crassa contains over 10 000 protein- coding genes. Gene knockouts or mutations in specific sequences may now be readily correlated with the observed cytological defects in the sexual stage, thus advancing our molecular understanding of complex processes during ascus and ascospore development.

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Section: Spore Killers As Meiotic Drive Elementsmentioning

confidence: 96%

Section: Natural Populationsmentioning

confidence: 99%

Neurospora as a model fungus for studies in cytogenetics and sexual biology at Stanford

Raju

2009

J Biosci

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“…One isolate from Europe (10866 Italy), however associated with clade A (predominantly representing isolates from the Caribbean Basin and the Ivory Coast; Jacobson et al, 2006). An explanation for this exception may be that isolate 10866 was at some time in history transported from the Caribbean Basin or Ivory Coast to Europe (e.g., by human trade).…”

Section: Resultsmentioning

confidence: 99%

Phylogenic analysis of additional Neurospora crassa isolates

Radic¹,

Gastaldello²,

Diegmann³

et al. 2012

Fungal Genetics Reports

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The ascomycete Neurospora crassa is classical model organisms in biology. So far, a phylogenetic analysis based on genomic sequences of four non-functional nuclear loci has been reported for 44 natural isolates of N. crassa. Three subgroups (clades) with a distinct geographical distribution have been identified: clade A (Caribbean Basin and Ivory Coast), clade B (Europe, Ivory Coast and India), and clade C (India). Here, we report the results of a phylogenetic analysis of 16 additional isolates. Six of these were from the Caribbean Basin, eight from Europe and one from Pakistan and one from Thailand. The previously described clades and their geographical distribution were generally confirmed. All Caribbean isolates belonged to clade A and all European isolates belonged to clade B, with the exception of one isolate from Italy, which also belonged to clade A, suggesting a transport from the Caribbean Basin or the Ivory Coast to Europe. Interestingly, the isolates from Pakistan and Thailand were found in a separate group, basal to all other clades. Their phylogenetic classification is not yet clear as they might belong to N. crassa but as well to N. perkinsii, potentially representing yet undescribed phylogenetic groups or species of Neurospora, or hybrids.

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“…Refl ecting the natural distribution of species, the FGSC collection holds 279 wild-type N. crassa isolates, 611 N. intermedia isolates, 193 N. tetrasperma isolates, 152 N. discreta isolates, 246 N. sitophila isolates and 12 isolates of other Neurospora species. Recent studies have shown that Neurospora can be found in temperate zones including the US and several countries in Europe (Jacobson et al 2006), and many of these temperate isolates have also been deposited in the FGSC collection. The wild strains in the FGSC collection have been used in genome sequencing efforts (table 2), and have allowed the identifi cation of the only transposon ever found in Neurospora (Sewell and Kinsey 1996).…”

Section: Fgsc Holdingsmentioning

confidence: 99%

The Fungal Genetics Stock Center: a repository for 50 years of fungal genetics research

2010

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The Fungal Genetics Stock Center (FGSC) was established in 1960 to ensure that important strains used in early genetics research were available to subsequent generations of fungal geneticists. Originally, only mutant strains were held. At present, any organism that has had its genome sequenced is a genetic system and so the FGSC has added many new organisms. The FGSC is well integrated in its core community and, as research came to depend on cloned genes, vectors and gene libraries, the FGSC included these materials. When the community expanded to include plant and human pathogens, the FGSC adopted these systems as well. Wild isolates from around the world have also proven instrumental in answering important questions. The FGSC holds tremendous diversity of the Neurospora species, which form the core of the collection. The growth in the number of strains distributed illustrates the growth in research on fungi. Because of its position near the centre of the fungal genetics effort, the FGSC is also the first to see trends in research directions. One recent example is the 300% jump in requests for strains of Neurospora crassa carrying a mutation that makes them sensitive to high salt concentration. These strains were seldom requested over many years, but became among our most popular resources following the demonstration of their utility in studying fungicide resistance. This exemplifies why materials need to be preserved without regard to their immediate perceived value and reinforces the need for long-term support for preservation of a broad variety of genetic resources.

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New findings of Neurospora in Europe and comparisons of diversity in temperate climates on continental scales

Abstract: The life cycles of the conidiating species of

Cited by 57 publications

References 19 publications

Neurospora as a model fungus for studies in cytogenetics and sexual biology at Stanford

Neurospora as a model fungus for studies in cytogenetics and sexual biology at Stanford

Phylogenic analysis of additional Neurospora crassa isolates

The Fungal Genetics Stock Center: a repository for 50 years of fungal genetics research

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