New experimental model of exposure to environmental tobacco smoke

Abstract

PURPOSE:To describe a new model to passive smoking for rodents. METHODS:Twenty rats were distributed into two study groups (N=10): control group (CG), that was not exposed to tobacco smoke and used as normal standard for biochemical and histological analysis; Experimental Group (EG), that Animals were exposed to the passive smoking; Euthanasia was performed after 14 days of exposure. The serum level of nicotine and histological analysis were performed. RESULTS:There was a statistical difference on the nicotine serum levels between Experimental and Control group, with level of 286 ±23 nanograma/mL in the EG and undetectable on CG (p<0.01). The histological study suggested the model efficacy producing alveolar destruction and emphysema in the EG compared with the insignificant lesions in the CG`s lung. CONCLUSION:The model of exposure to environmental tobacco smoke for rodents induced easily the changes related to secondhand smoke.

Action of a deproteinized xenogenic biomaterial in the process of bone repair in rats submitted to inhalation of cigarette smoke

“…Exposure of the animal to nicotine can be accomplished in a number of ways, varying both in times of exposure as well as in the passive inhalation device 21 . In this experiment, the option was made to use the method proposed by Cendon et al 22 because, due to its simplicity, low cost and short duration, this technique proved to be a useful model for experiments in the area and animal model used.…”

Changes of vitamin D receptors (VDR) and MAPK activation in cytoplasmic and nuclear fractions following exposure to cigarette smoke with or without filter in rats

“…Rats in each group were sub-divided into 7 and 14 days of exposure groups (n ¼ 5 in each subgroup). The exposure system was composed of five components; air compressor, cigarette filter articulation, the inhalation chamber, cigarette container, ash and smoke storage box (Figure 1) as previously described [14].…”