New developments reproductive technologies in deer

Berg, D.K.; Asher, G. W.

doi:10.1016/s0093-691x(02)01272-4

Cited by 45 publications

(29 citation statements)

References 33 publications

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“…Following the electrical stimulus, the reconstructed embryos were washed in H-DSOF þ 10% FCS and checked for fusion by microscopic examination 30 min later. Reconstructed SCNT embryos were artificially activated 3.5 h (range: 3-5.5 h) after fusion, using a combination of 5 lM ionomycin in HEPES-buffered IVF-DSOF [58] for 5 min and 2 mM 6-dimethylaminopurine in early DSOF for 3 h. Following three washes in H-DSOF, SCNT reconstructs were placed into in vitro culture (IVC).…”

Section: Nt and Artificial Activationmentioning

confidence: 99%

Red Deer Cloned from Antler Stem Cells and Their Differentiated Progeny1

Berg¹,

Li²,

Asher³

et al. 2007

Biology of Reproduction

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The significance of donor cell differentiation status for successful cloning by somatic cell nuclear transfer (SCNT) is unclear. Here, we cloned a new species, red deer (Cervus elaphus), from multipotent antler stem cells and their differentiated progeny. Cultured donor cell lines from male antlerogenic periosteum (AP) were left undifferentiated or chemically induced to initiate osteogenesis or adipogenesis. Based on their morphology and marker gene expression profile, donor cells were classified as undifferentiated AP cells, presumptive osteoblasts, or adipocytes. Adipocytes upregulated adipogenic markers procollagen type I alpha 2 (COL1A2), peroxisome proliferator-activated receptor gamma 2 (PPARG), and gylceraldehyde-3-phosphate dehydrogenase (GAPDH), and downregulated antlerogenic transcripts POU-domain class 5 transcription factor (POU5F1) and parathyroid hormone (PTH)-like hormone (PTHLH). Despite differences prior to NT, transcript abundance of donor-specific markers COL1A2, PPARG, GAPDH, and POU5F1 did not differ significantly in cloned blastocysts (P = 0.10, 0.50, 0.61, and 0.16, respectively). However, donor cell and blastocyst expression levels were completely different for most genes analyzed, indicating their successful reprogramming. The type of donor cell used for NT (AP, bone, and fat cells), had no effect on in vitro development to blastocysts (93 [38%] of 248 vs. 32 [44%] of 73 vs. 59 [32%] of 183, respectively). Likewise, development to weaning was not significantly different between the three cell types (2 [4%] of 46 vs. 2 [29%] of 7 vs. 4 [13%] of 31, for AP vs. bone vs. fat, respectively). Microsatellite DNA analysis confirmed that the eight cloned red deer calves were genetically identical to the cells used for NT.

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Section: Nt and Artificial Activationmentioning

confidence: 99%

Red Deer Cloned from Antler Stem Cells and Their Differentiated Progeny1

Berg¹,

Li²,

Asher³

et al. 2007

Biology of Reproduction

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“…The resulting mature oocytes are therefore of inferior quality when compared to in vivo matured oocytes. In seasonal animals, oocytes collected out of the season may show resistance to IVM and IVF (Spindler et al, 2000;Berg & Asher, 2003;Comizzoli et al, 2003). In red deer for example, while about 15% of cleaved oocytes collected during the season (April-July) developed in vitro to blastocysts, none have developed if collected after July (Berg & Asher, 2003).…”

Section: Oocyte Cryopreservationmentioning

confidence: 99%

“…In seasonal animals, oocytes collected out of the season may show resistance to IVM and IVF (Spindler et al, 2000;Berg & Asher, 2003;Comizzoli et al, 2003). In red deer for example, while about 15% of cleaved oocytes collected during the season (April-July) developed in vitro to blastocysts, none have developed if collected after July (Berg & Asher, 2003). Comizzoli et al (2003) showed that anti-oxidants and FSH in the culture media can overcome this problem in the domestic cat model they have studied.…”

Section: Oocyte Cryopreservationmentioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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“…In terms of maturational ability, oocytes collected during the quiescent season(s) of the year are likely to be resistant to development in vitro. Examples of this phenomenon are already available from the domestic cat (Spindler et al 2000;Comizzoli et al 2003) and red deer (Berg and Asher 2003), wherein oocytes recovered in the non-breeding season are compromised compared with those evaluated during the peak breeding time of the year. The result is lower or non-existent embryo production during most of the year.…”

Section: Based On In Vivo-and In Vitro-matured Oocytesmentioning

confidence: 99%

Applications of emerging technologies to the study and conservation of threatened and endangered species

Pukazhenthi

Comizzoli

Travis

et al. 2006

Reprod. Fertil. Dev.

140

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Abstract. Sustaining viable populations of all wildlife species requires the maintenance of habitat, as well as an understanding of the behaviour and physiology of individual species. Despite substantial efforts, there are thousands of species threatened by extinction, often because of complex factors related to politics, social and environmental conditions and economic needs. When species become critically endangered, ex situ recovery programmes that include reproductive scientists are the usual first line of defence. Despite the potential of reproductive technologies for rapidly increasing numbers in such small populations, there are few examples of success. This is not the result of a failure on the part of the technologies per se, but rather is due to a lack of knowledge about the fundamental biology of the species in question, information essential for allowing reproductive technologies to be effective in the production of offspring. In addition, modern conservation concepts correctly emphasise the importance of maintaining heterozygosity to sustain genetic vigour, thereby limiting the practical usefulness of some procedures (such as nuclear transfer). However, because of the goal of maintaining all extant gene diversity and because, inevitably, many species are (or will become) 'critically endangered', it is necessary to explore every avenue for a potential contributory role. There are many 'emerging technologies' emanating from the study of livestock and laboratory animals. We predict that a subset of these may have application to the rescue of valuable genes from individual endangered species and eventually to the genetic management of entire populations or species. The present paper reviews the potential candidate techniques and their potential value (and limitations) to the study and conservation of rare wildlife species.

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New developments reproductive technologies in deer

Cited by 45 publications

References 33 publications

Red Deer Cloned from Antler Stem Cells and Their Differentiated Progeny1

Red Deer Cloned from Antler Stem Cells and Their Differentiated Progeny1

Genome Banking for Vertebrates Wildlife Conservation

Applications of emerging technologies to the study and conservation of threatened and endangered species

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