New Data on Variously Directed Dose-Response Relationships and the Combined Action Types for Different Outcomes of <i>in Vitro</i> Nanoparticle Cytotoxicity

Panov, Vladimir G.; Бушуева, Т. В.; Minigalieva, Ilzira A.; Naumova, Anna; Shur, V. Ya.; Шишкина, Е. В.; Sutunkova, Marina P.; Gurviсh, Vladimir; Privalova, Larisa I.; Katsnelson, Boris A.

doi:10.1177/15593258211052420

Cited by 5 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that the vast majority (85.7%) of dose-response relationships observed after exposure to various pollutants are nonlinear [51]. Such polyphase links have been repeatedly shown in in vitro experiments [52,53]. Thus, according to the main cytological indicator, i.e., the ratio of neutrophilic leukocytes to alveolar macrophages, the highest response following exposure to PbO NPs (Figure 8d) occurred at a dose of 0.2 mg per rat, which was not the highest among those tested by us.…”

Section: Relationship Between the Dose Of Nanoparticles And Their Cyt...mentioning

confidence: 90%

Comparative Evaluation of the Cytotoxic Effects of Metal Oxide and Metalloid Oxide Nanoparticles: An Experimental Study

Sutunkova

Klinova

Ryabova

et al. 2023

IJMS

View full text Add to dashboard Cite

Industrial production generates aerosols of complex composition, including an ultrafine fraction. This is typical for mining and metallurgical industries, welding processes, and the production and recycling of electronics, batteries, etc. Since nano-sized particles are the most dangerous component of inhaled air, in this study we aimed to establish the impact of the chemical nature and dose of nanoparticles on their cytotoxicity. Suspensions of CuO, PbO, CdO, Fe2O3, NiO, SiO2, Mn3O4, and SeO nanoparticles were obtained by laser ablation. The experiments were conducted on outbred female albino rats. We carried out four series of a single intratracheal instillation of nanoparticles of different chemical natures at doses ranging from 0.2 to 0.5 mg per animal. Bronchoalveolar lavage was taken 24 h after the injection to assess its cytological and biochemical parameters. At a dose of 0.5 mg per animal, cytotoxicity in the series of nanoparticles changed as follows (in decreasing order): CuO NPs > PbO NPs > CdO NPs > NiO NPs > SiO2 NPs > Fe2O3 NPs. At a lower dose of 0.25 mg per animal, we observed a different pattern of cytotoxicity of the element oxides under study: NiO NPs > Mn3O4 NPs > CuO NPs > SeO NPs. We established that the cytotoxicity increased non-linearly with the increase in the dose of nanoparticles of the same chemical element (from 0 to 0.5 mg per animal). An increase in the levels of intracellular enzymes (amylase, AST, ALT, LDH) in the supernatant of the bronchoalveolar lavage fluid indicated a cytotoxic effect of nanoparticles. Thus, alterations in the cytological parameters of the bronchoalveolar lavage and the biochemical characteristics of the supernatant can be used to predict the danger of new nanomaterials based on their comparative assessment with the available tested samples of nanoparticles.

show abstract

Section: Relationship Between the Dose Of Nanoparticles And Their Cyt...mentioning

confidence: 90%

Comparative Evaluation of the Cytotoxic Effects of Metal Oxide and Metalloid Oxide Nanoparticles: An Experimental Study

Sutunkova

Klinova

Ryabova

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…This combination is of particular scientific interest because both of these elements are essential and, therefore, exposure to nanoparticles of these species may be expected to cause not only harmful but also beneficial dose-dependent responses of the organism. Nevertheless, previously we demonstrated their cytotoxicity on a culture of fibroblast-like cells 1 as assessed by the effect of ATP-dependent chemoluminescence inhibition.…”

Section: Introductionmentioning

confidence: 98%

Dose Dependence of the Separate and Combined Impact of Copper-Oxide and Selenium-Oxide Nanoparticles on Oxygen Consumption by Cells In Vitro With or Without the Background Action of Some Modulators of the Mitochondrial Respiratory Function

et al. 2023

Self Cite

View full text Add to dashboard Cite

We had previously demonstrated on various stable cell cultures exposed to chemically different nanoparticles when assessing their cytotoxicity by different outcomes, dose-response relationships may be either monotonic or non-monotonic falling within an extended understanding of the hormesis paradigm. Presently, on human fibroblasts exposed to the copper-oxide and/or selenium-oxide nanoparticles, we assessed their cytotoxic effect by the inhibition of oxygen uptake against modulating the respiratory function of mitochondria (oligomycin, followed by carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone, and, finally, rotenone with antimycin A). It is hypothesized that a given type of this relationship is closely associated with the mitochondrial respiratory function. However, in only one case, this hypothesis was confirmed by finding that the monotonic dose-response relationship transformed into a non-monotonic one of the hormesis type under exposure to SeO-NP with the mitochondrial respiratory function fully inhibited by the effect of rotenone + antimycin А. In all other variants of the experiment, the shape of this relationship did not depend on the addition of the above agents to the cell culture. Neither did the effect of these modulators change the pattern of combined action of CuO-NP and SeO-NP, which was additive in all cases.

show abstract

The hormetic response of heart rate of fish embryos to contaminants – Implications for research and policy

Evgenios

2022

Science of The Total Environment

View full text Add to dashboard Cite

New Data on Variously Directed Dose-Response Relationships and the Combined Action Types for Different Outcomes of in Vitro Nanoparticle Cytotoxicity

Cited by 5 publications

References 13 publications

Comparative Evaluation of the Cytotoxic Effects of Metal Oxide and Metalloid Oxide Nanoparticles: An Experimental Study

Comparative Evaluation of the Cytotoxic Effects of Metal Oxide and Metalloid Oxide Nanoparticles: An Experimental Study

Dose Dependence of the Separate and Combined Impact of Copper-Oxide and Selenium-Oxide Nanoparticles on Oxygen Consumption by Cells In Vitro With or Without the Background Action of Some Modulators of the Mitochondrial Respiratory Function

The hormetic response of heart rate of fish embryos to contaminants – Implications for research and policy

Contact Info

Product

Resources

About