New Bioactive Fungal Molecules with High Antioxidant and Antimicrobial Capacity Isolated fromCerrena unicolorIdiophasic Cultures

Abstract

Three bioactive fractions, extracellular laccase (ex-LAC), crude endopolysaccharides (c-EPL), and a low molecular subfraction of secondary metabolites (ex-LMS), were isolated from the idiophasic cultures of the white rot fungus Cerrena unicolor. For the first time, we determined the antioxidant properties of these samples by chemiluminometric measurement (a) and assessment of the scavenging effect on ABTS (b) and the DPPH reduction rate (c). The highest reducing capability was found for the ex-LMS fraction: 39–90% for (a), 20–90% for (b), and 10–59% for (c) at the concentration of 6.25–800 µg/mL. The scavenging abilities of the C. unicolor c-EPL were between 36 and 70% for (a), 2 and 60% for (b), and 28 and 32% for (c) at the concentration of 6.25–800 µg/mL. A very high prooxidative potential was observed for the ex-LAC probes. The preliminary toxicity tests were done using the Microtox system and revealed the following percentage of the toxic effect against Vibrio fischeri: 85.37% for c-EPL, 50.67% for ex-LAC, and 99.8% for ex-LMS, respectively. The ex-LAC sample showed the antibacterial activity against Escherichia coli, c-EPL against Staphylococcus aureus, and ex-LMS against both bacterial strains, respectively, but the stronger inhibitory effect was exerted on S. aureus.

Dennettia tripetala seeds inhibit ferrous sulphate-induced oxidative stress in rat tissues in vitro

“…The antiradical scavenging activity of the D. tripetala aqueous seed extracts mentioned in this study is owing to the presence of phenols that usually possesess high metal-chelating activity. This result correlates with the reports of Jazek et al [30], Sadi et al, [31] and Ojo et al [32,33]. Tables 1 and 2].…”

Fungus Cerrena unicolor as an effective source of new antiviral, immunomodulatory, and anticancer compounds

“…the subject of the present work, had been reported mainly as a very efficient laccase producer [8]. Recently, appropriate data describing the possibility of using this species as a source of biologically active fractions have also been published [4]. Anti-or prooxidative potential and antimicrobial activities were described for three fractions: (a) proteins: represented by laccase (LAC) (b) carbohydrate: represented by crude endopolysaccharides (c-EPS) and (c) low molecular weight components: represented by sub-fraction of extracellular secondary metabolites (ex-LMS).…”

“…It seems that fungi occupy a special place in the extremely wide range of organisms used in the studies of carcinogenesis. Numerous fungal bioactive compounds including proteins, low molecular weight substances, polysaccharides, or polysaccharide-protein complexes have been isolated and investigated towards their antitumor, immunomodulatory as well as antioxidative, antiviral, and antimicrobial effects [1][2][3][4]. Wood decaying fungi belonging to the phyllum Basidiomycetes have become one of the most interesting groups of organisms used in red biotechnology [2].…”

An extracellular laccase with potent dye decolorizing ability from white rot fungus Trametes sp. LAC-01

“…It was first obtained from the Japanese lacquer tree (Rhus vernicifera) in 1883 and subsequently isolated from fungi in 1896, which makes it one of the oldest enzymes ever described [2]. Laccases are widely distributed in plants, bacteria, insects, and especially fungi [3,4]. They can be roughly divided into two major groups, i.e.…”