New Amphiphilic Branched Copolymers of N-Vinylpyrrolidone with Methacrylic Acid for Biomedical Applications

New Nanosized Systems Doxorubicin—Amphiphilic Copolymers of N-Vinylpyrrolidone and (Di)methacrylates with Antitumor Activity

“…The copolymer ability to temperature respond was maintained in slightly acidic (рН 5) and neutral (рН 6.8-7.4) media, in contrast to the VP-MAA-TEGDM terpolymer. The intensity of light scattering by neutral/weakly alkaline and alkaline aqueous solutions of VP-MAA-TEGDM in the studied temperature interval remained almost invariable, and polymer particles with the hydrodynamic radius smaller than 10 nm were presenting in these solutions [52]. Here, we showed DLS data for the nanosized DOX system based on this terpolymer in water, i.e., slightly acidic medium.…”

“…We used a VP-TEGDM copolymer (CPL1) obtained by radical copolymerization in toluene at a monomer molar ratio of 100:5 as described in [25], and a VP-MAА-TEGDM copolymer (CPL2) synthesized from a 98:2:2 monomer mixture in ethanol [52]. The copolymers were isolated from the reaction mixture by precipitation with a 10-fold excess of hexane.…”

“…The copolymers were isolated from the reaction mixture by precipitation with a 10-fold excess of hexane. The obtained copolymers were described in detail elsewhere [42,52]. The aim of this work is to prepare and characterize via different physical chemical methods new nanosized DOX systems based on amphiphilic branched copolymers of N-vinylpyrrolidone with triethylene glycol dimethacrylate (VP-TEGDM), and VP-MAA-TEGDM where MAA is methacrylic acid, and to perform quantum-chemical modeling of the structure of DOX-copolymer complexes and study the biological activity of prepared structures in vitro on HeLa tumor cells.…”

“…The copolymers were isolated from the reaction mixture by precipitation with a 10-fold excess of hexane. The obtained copolymers were described in detail elsewhere [42,52].…”

“…The copolymer synthesis is complicated, and its cost is rising; therefore, special cleaning methods are required. The branched nature of the VP-MAA-TEGDM copolymer was demonstrated in [52] through an analysis of the dependence of M w on the eluent volume V r and root-mean-square radii of gyration on M w values and comparison with those for linear PVP. The VP-TEGDM copolymer was characterized by IR spectroscopy and NMR spectroscopy [53].…”