New Alkaloids of Claviceps paspali

New aspects of submerged fermentation of Claviceps paspali

“…2). Paspalic acid is isomerized to lysergic acid which gives rise to LAH I. Degradation of this substance affords ergine, which is converted to 8-hydroxyergine in a biooxidation reaction described earlier (Flieger et al 1989a). PAH and paspalic acid amide have not yet been determined and their existence is assumed.…”

“…Total alkaloids were determined in fermentation broth colorimetrically with the van Urk reagent (Robbers et al 1972). Analytical and semipreparative liquid chr~omatography was performed on a Perkin-Elmer (U.S.A.) instrument with gradient elution as described previously (Flieger et al 1989a).…”

“…Isolation, semipreparative HPLC, MS, 1H-and ~3C-NMR spectrometry were performed as described earlier (Flieger et al 1989a). Mass spectra contained an ion of m/z 284 with the ele- ..... ) during fermentation with Claviceps paspali MG-6 terized by the formation of LAH I and its epimeric products LAHs II, III, IV .…”

Detection of ergot alkaloids from Claviceps species in agricultural products by competitive ELISA using a monoclonal antibody

“…Vining (1973) listed 46 ergot alkaloids as having been identified in Claviceps species, including ergoline and lysergic acid derivatives. Since that time, the number of ergot alkaloids reported has grown as new members of the group have been added (Flieger et al, 1984(Flieger et al, , 1989. The ergot alkaloids include many with profound pharmacological activities which can be beneficial when administered in the proper context or derivatized or which can be toxic to humans and livestock when ingested in agricultural commodities (Floss et al, 1973).…”

Improved Method of Analysis for Ergovaline in Tall Fescue by High-Performance Liquid Chromatography

“…Since the ergot compounds with a double bond at position 9,10 fluoresce strongly in UV light, fluorescence detection offers both enhanced sensitivity and decreased background interference in the chromatogram, when compared to other methods of detection. HPLC has been used to detect ergot compounds in wheat or rye (Fajardo et al, 1994;Scott and Lawrence, 1980;Ware et al, 1986), pharmaceutical preparations (Cieri, 1987), plasma (Elund, 1981), and cultures (Flieger et al, 1989). The method was also adapted to detect ergovaline in tall fescue seeds and forage (Hill et al, 1993;Rottinghaus et al, 1991;Craig et al, 1994;Welty et al, 1994;Yates and Powell, 1988).…”