Neutralizing antibodies against Omicron BA.4/5 after COVID-19 vaccination in SARS-CoV-2 experienced versus naïve individuals in the general population

A machine learning model for predicting serum neutralizing activity against Omicron SARS‐CoV‐2 BA.2 and BA.4/5 sublineages in the general population

“…Anti‐SARS‐CoV‐2 nucleocapsid protein (N) IgG antibodies and anti‐RBD total antibodies were measured by the Elecsys® Anti‐SARS‐CoV‐2 N IgG assay and the Elecsys® Anti‐SARS‐CoV‐2 S assay (Roche Diagnostics), as previously reported 17 . NtAbs against Omicron BA.2 and BA4/5 were measured using a GFP‐expressing vesicular stomatitis virus pseudotyped with the Omicron BA.2 and BA.4/5 S protein, as described elsewhere 18–20 …”

“…17 NtAbs against Omicron BA.2 and BA4/5 were measured using a GFP-expressing vesicular stomatitis virus pseudotyped with the Omicron BA.2 and BA.4/5 S protein, as described elsewhere. [18][19][20]…”

Determinants of reinfection with SARS-CoV-2 Omicron variant

“…We read with interest the study published by Camacho et al in the Journal of Infection showing that individuals who were vaccinated prior to SARS-CoV-2 infection displayed lower neutralizing antibody titers against both Wuhan-Hu-1 and Omicron BA.4/5 variants than individuals who were vaccinated after experiencing SARS-CoV-2 infection irrespective of when SARS-CoV-2 infection was documented. 1 These findings could imply that vaccination after infection provided better protection against Omicron variant reinfection than vaccination before first infection. Nevertheless, a recent study that addressed the magnitude of protection conferred by previous infection against Omicron variant reinfection found that both previous infection (alone or combined with previous vaccination) conferred rapidly waning protection against reinfection with the omicron variant.…”