Neuroprotective Role of Heat Shock Protein 70 in the Rostral Ventrolateral Medulla during Acute Mevinphos Intoxication in the Rat

Chang, Chia‐Hung; Chang, Alice Y.W.; Chan, Samuel H.H.

doi:10.1159/000081821

Cited by 3 publications

(2 citation statements)

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“…Indeed, a number of the heat shock proteins (HSPs) are affected by both anaesthetics and, given that HSP70, HSP84b and HSP 90 are known to have neuroprotective roles (7,23,25,26,35,36), this could pose significant risks to neurons during and after anaesthesia. Modulation of proteins like syntaxin B2 and dihydropyrimidinaselike proteins, which are involved in synaptic structure and function (1,2,18,21,22,39), could also have long-term effects on brain circuitry, so it is vital that we understand the outcomes of using particular anaesthetic agents.…”

Section: Discussionmentioning

confidence: 99%

Propofol anaesthesia alters the cerebral proteome differently from sevoflurane anaesthesia

Tsuboko

Sakamoto

2011

Biomed. Res.

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Previous studies suggest that propofol and sevoflurane anaesthesia in rats may have variable effects on the proteome. Brains from untreated rats and rats anaesthetised with intravenous propofol infusion or inhaled sevoflurane were collected at various time points post-anaesthesia and subjected to global protein expression profiling using two-dimensional gel electrophoresis. Significant changes in protein spot intensity (i.e. expression) between the propofol and sevoflurane groups demonstrated clear similarities and differences in proteomic regulation by these anaesthetics. The proteins regulated were broadly classified into groups involved in cytoskeletal/neuronal growth, cellular metabolism, signalling, and cell stress/death responses. Proteins concerned with cell death and stress responses were down-regulated by both agents, but the anaesthetics had variable effects on proteins in the other groups. Importantly, proteins such as Ulip2 and dihydropyrimidinaselike-2 were regulated in opposite directions by propofol and sevoflurane. Moreover, the timecourse of regulation of proteins varied depending on the agent used. These data suggest different underlying mechanisms of proteomic regulation. We found that sevoflurane anaesthesia had more pronounced effects, on a wider range of proteins, and over an apparently longer duration than propofol. Thus, sevoflurane could be considered a more disruptive anaesthetic agent. Our findings show that protein expression is regulated differentially according to the anaesthetic agent and the method of delivery support and extend our previous observations of differential genomic regulation by anaesthetics in the brain. This study highlights the power of proteomic studies in assessing the effects of certain anaesthetics on the integrity of neuronal structure and function.The utility of general anaesthetics is unquestioned, and their harmlessness with regard to mortality and morbidity has been evaluated and endorsed by clinical outcomes (3,(10)(11)(12)24). Little is known, however, regarding their comprehensive influence at the genomic or proteomic level, which is not reflected in mortality and morbidity. A number of genomic studies have been published, and we have shown that general anaesthesia alters gene expression, especially expression of genes involved in circadian rhythms and drug metabolism. For instance, we found persistent suppression of the expression of several genes implicated in circadian rhythms (e.g., Per2, Dbp, Egr1, Krox20 and NGF1-B) following treatment of rats with sevoflurane (20) and propofol or dexmedetomidine (41). We have also reported changes in the expression of drug metabolising enzymes in rats (e.g., Cyp2b15, Por, Nr1i2, Ces2, Ugt1a7, Abcb1a and Abcc2) in response to sevoflurane, isoflurane, propofol, or dexmedetomidine anaesthesia (30, 37) The observed changes varied depending on the identity of the anaesthetic used, and also on the mode of administration (inhaled vs. intravenous). Therefore, it is clear that anaesthetics have significant effects...

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Section: Discussionmentioning

confidence: 99%

Propofol anaesthesia alters the cerebral proteome differently from sevoflurane anaesthesia

Tsuboko

Sakamoto

2011

Biomed. Res.

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“…Within the HSP family, HSP70 is the major inducible member that confers protection against cell death [56]. Chang et al [6] investigated whether HSP70 plays a neuroprotective role at the rostral ventrolateral medulla (RVLM), the origin of sympathetic neurogenic vasomotor tone in the medulla oblongata where the organophosphate insecticide mevinphos (Mev) acts to elicit cardiovascular toxicity [73]. Mev induces an increase in HSP70 level in the rat ventrolateral medulla.…”

Section: Neuroprotection By Hsp70 Against Organophosphate Intoxicationmentioning

confidence: 99%

Biomedical Vignette

2004

J Biomed Sci

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Biomedical vignette

2004

J Biomed Sci

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Although non-invasive identification of specific cerebral surface structures is crucial to clinical diagnosis and intervention, conventional MRI often fails to provide satisfactory outcome data due to the crude resolution of the tomographic slices [23]. Gong et al. [23] reported a new technique using MR-traced diffusion-weighted imaging that displays clear structures of the cerebral surface in normal subjects. They also obtained excellent results in mapping cerebral infarctions and their relation with cortical sulci/gyri in patients with acute or chronic cerebral infarcts. The high level of clarity in threedimensional visualization of cerebral surface structures offered by this new technique provides an additional dimension for diagnostic assessment and biomedical research, Dextromethorphan Potentiates Morphine-Induced AntinociceptionThe non-opioid antitussive, dextromethorphan, potentiates the analgesic effect of morphine and decreases the requirement of morphine for acute postoperative pain in rats [28] and patients [13]. Chow et al.[I6] studied the pharmacological mechanism that underlies these actions. Whereas potentiation of dextromethorphan on morphine-induced antinociception is observed via both intrathecaI and intracerebroventricular routes, neither 5-HT2 receptor nor %-adrenergic receptor antagonist elicits significant effect on this potentiation. These results suggest that serotoninergic or adrenergic descending paininhibitory pathways do not mediate the potentiating effect of dextromethorphan. Opioid Receptors Participate in Cardioprotection Induced by Cold-Restraint Stress or MorphineCold exposure is known to confer cardioprotection against ischemic insults. Whereas g-opioid receptor has been implicated in this process [33], the contribution of K-and b~-opioid receptors remains to be elucidated. Wu et aI. [70] demonstrated that cardioprotection conferred by cold and restraint stress or morphine is abolished by pretreatment with opioid receptor antagonists. Inhibition ot protein kinase C or blockade of mitochondrial KArp channel (both are hmwn [38,45] to play a role in preconditioning-induced cardioprotection) also reduces infarct size induced by myocardial ischemia/reperfusion. These results indicate the participation of all three opioid receptors, protein kinase C and mitochondriat KAr~ channel in cardioprotection induced by cold-restraint stress in the rat. Involvement of the Locus Ceruleus in Behavioral Changes Associated with Organophosphate InsecticidesExposures to organophosphate insecticides induce undesirable behavioral changes in humans, including anxiety and irritability, depression, cognitive disturbances, and sleep disorders [35]. Zhu et al.[75] delineated the underlying neural mechanisms by studying the role of the locus ceruleus (LC), a pontine site that contains high levels of acetylcholinesterase and is involved in regulation of the sleep-wake cycIe, attention, arousal, memory, and pathological processes, including anxiety and depression [22]. They reported that methyl parathion, a co...

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Neuroprotective Role of Heat Shock Protein 70 in the Rostral Ventrolateral Medulla during Acute Mevinphos Intoxication in the Rat

Cited by 3 publications

References 29 publications

Propofol anaesthesia alters the cerebral proteome differently from sevoflurane anaesthesia

Propofol anaesthesia alters the cerebral proteome differently from sevoflurane anaesthesia

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