Neuroprotective Effect of Hydroalcoholic Extract of Areca Catechu Linn on β-Amyloid (25-35) Induced Cognitive Dysfunction in Mice

Abstract

Alzheimer's disease is the most common form of dementia in elderly. There is currently no cure for Alzheimer's disease. But some category of drugs like AchE inhibitors and NMDA antagonists were used along with some antioxidants and some other supportive therapy. There is a possibility to slow down the brain's degeneration caused by Alzheimer's with natural treatments. In the present study animals were divided randomly into five groups of six animals each. Group I animals were given 0.1 % w/v CMC orally by using intra-gastric catheter at dose (10 ml/kg), Group III and Group IV animals were pretreated with hydroalcoholic extract of Areca catechu Linn (HAEAC) for a period of 3 weeks (200 and 400 mg/ kg b.w) and Group V animals were treated with donepezil (1.5 mg/kg/b.w i.p) and were kept in light/dark cycle. During this period the animals were trained in water-maze, Y-maze, exploratory behaviour and passive avoidance apparatus for memory. Amnesia is induced by intra cerebro ventricular injection (I.C.V) of βamyloid (25-35). I.C.V injection for the 2 nd , 3 rd , 4 th and 5 th groups were performed on the 21 st day of the pretreated animals and continued for 5 days. The last dose was given 60 minutes prior to behavioral testing and on 30 th day scarification of animals was done for in-vitro studies. Hydroalcoholic extract of Areca catechu Linn showed significant protective effect on neurodegeneration and also showed improvement on memory retention activity when compared with β-amyloid (25-35) induced animals (Group II).

Investigation of anti-Alzheimer’s activity of aqueous extract of areca nuts (Areca catechu L.): In vitro and in vivo studies

“…The role of arecoline in increasing the level of acetylcholine in the Central Nervous System (CNS) of animals and AD patients have been fully discussed in the literature (Peng et al, 2015;Volgin et al, 2019). Also, significant neuroprotective activity of hydroalcoholic extract of betel nuts at a dose of 200 mg/kg on Aβ-induced cognitive dysfunction in mice has been reported by Kannan et al (2013). However, other biological activities involved in AD have not been investigated and in this study, they were evaluated.…”

Monoamine Oxidase Inhibitory Effects of Medicinal Plants in Management of Alzheimer's Disease

“…Ethanolic extract of arecanut (4-80 mg/kg) inhibits monoamine oxidase in albino rats (64). In mice brain, treatment of 200 and 400 mg/kg hydroalcoholic extract of arecanut reduce MAO level (65). The dichloromethane fraction of arecanut elevates serotonin and dopamine in the brain by inhibiting MAO-A (66).…”

Cognitive Enhancing, Anti Acetylcholinesterase and Antioxidant Properties of Tagetes Erecta Against Diazepam Induced Amnesia in Rodents

“…The movement of animal interrupts a beam of light falling on a photocell during which a count was recorded and displayed. [15] Cook's pole climbing method The basic operational mode of this method is that following an auditory warning stimulus, the animal learns to avoid the foot shock delivered through the cage floor by jumping to pole. This method has long been accepted as a reliable technique to evaluate learning and memory in experimental animals.…”