Neuropilin-1 distinguishes natural and inducible regulatory T cells among regulatory T cell subsets in vivo

Abstract

Neuropilin-1 is identified as a surface marker to distinguish different Foxp3+ T reg cell subsets under homeostatic conditions.

Differential Postselection Proliferation Dynamics of αβ T Cells, Foxp3+ Regulatory T Cells, and Invariant NKT Cells Monitored by Genetic Pulse Labeling

“…Up to now, no solid marker to discriminate thymus-derived and peripherally induced Tregs was found. Recently, Nrp-1 was proposed to be expressed at high levels on thymus-derived opposed to peripherally induced Tregs (40,41). In this study, we found lower H2BeGFP levels in Nrp1 high compared with Nrp1 low Tregs.…”

“…Next, we sought to investigate the proliferation history of Treg populations defined by Treg-specific markers. The transcription factor Helios (39) and the surface molecule Nrp1 (40,41) were recently proposed to discriminate thymic-derived and peripherally induced Tregs. As revealed by H2BeGFP levels of the respective Treg populations in secondary lymphoid organs, Helios + and Nrp1 high Tregs had proliferated more than Helios 2 or Nrp1 low Tregs (Fig.…”

The role of all-trans retinoic acid in the biology of Foxp3+ regulatory T cells

“…This suggests that the overwhelming majority of thymus-derived, natural Treg cells express Nrp-1. 27 Similarly, Helios provides an additional marker for the discrimination of nTreg cells from iTreg cells, although its specificity remains a concern. 28,29 Nrp-1 also identifies Foxp3 1 cell stability because Nrp-1 1 nTreg cells are more stable compared with Nrp-1 2 nTreg cells.…”

HCV epitope, homologous to multiple human protein sequences, induces a regulatory T cell response in infected patients

“…In mice, CD304 expression differentiates natural (CD304 + ), from inducible (CD304 -), T reg cells [22]. While a similar distinction has yet to be reported in humans, it is pertinent to note that the bulk of …”

“…This finding indicates that HCV_G1_p7_794 induces the conversion of conventional CD4 + FoxP3 -T cells to T reg cells, i.e., infectious tolerance, a suggestion supported by studies demonstrating the inability of nT reg cells to proliferate in response to their cognate antigen in vitro [34]. Furthermore, in contrast to Ab + VL + PBMCs cultured in medium alone, only a minority of CD3 + CD4 + FoxP3 + cells cultured in the presence of HCV_G1_p7_794 expressed CD304 (neuropilin), which is expressed by a subset of FoxP3 + T reg cells in humans and associated specifically with nT reg cells in mice [21,22]. While it has been suggested alternatively that the expanded T reg cell population in chronic, HCV infected patients is composed of cells phenotypically similar to nT reg or iT reg cells [11,20], our results concur with the consensus that the expanded T reg cell population in chronic HCV-infected patients is heterogeneous, composed of both T reg cell subsets.…”