Neuropeptide S Reinstates Cocaine-Seeking Behavior and Increases Locomotor Activity through Corticotropin-Releasing Factor Receptor 1 in Mice

Pañeda, Covadonga; Huitrón‐Reséndiz, Salvador; Frago, Laura M.; Chowen, Julie A.; Picetti, Roberto; Lecea, Luı́s de; Roberts, Amanda J.

doi:10.1523/jneurosci.5256-08.2009

Cited by 96 publications

(101 citation statements)

References 35 publications

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“…1,2 Recent evidence from rodent models indicates that the NPS system is critically involved in the regulation of unconditioned and conditioned fear behaviors. 1,[3][4][5][6][7] In humans, the locus for the NPS receptor gene NPSR1 maps to a chromosomal region that has reproducibly been linked to PD, with those markers creating the highest lod scores lying in close vicinity to he NPSR1 locus. [8][9][10] NPSR1 contains a frequent A>T polymorphism (rs324981) that codes for an Asn/Ile change at position 107.…”

Section: Introductionmentioning

confidence: 99%

A neuropeptide S receptor variant associated with overinterpretation of fear reactions: a potential neurogenetic basis for catastrophizing

et al. 2010

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Section: Introductionmentioning

confidence: 99%

A neuropeptide S receptor variant associated with overinterpretation of fear reactions: a potential neurogenetic basis for catastrophizing

et al. 2010

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“…In addition, Pañeda and coworkers reported that ICV NPS itself increased relapse-like behavior in mice previously trained to self-administer cocaine. This latter effect was blocked by CRF1R antagonism and was absent in CRF1 receptor KO mice, suggesting a stress-like effect of NPS under the experimental conditions used (5).…”

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confidence: 85%

Neuropeptide S facilitates cue-induced relapse to cocaine seeking through activation of the hypothalamic hypocretin system

Kallupi

Cannella

Economidou

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

102

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Drug addiction is a chronic relapsing disorder characterized by compulsive drug seeking and use. Environmental conditioning factors are among the major determinants of relapse in abstinent cocaine users. Here we describe a role of the neuropeptide S (NPS) system in regulating relapse. In rats with a history of cocaine selfadministration, presentation of stimuli predictive of drug availability reinstates drug seeking, triggering relapse. Intracerebroventricular (ICV) injection of NPS increased conditioned reinstatement of cocaine seeking, whereas peripheral administration of the NPS receptor antagonist SHA 68 reduced it. Manipulation of the NPS receptor system did not modify cocaine self-administration. We also found that ICV NPS administration activates c-Fos expression in hypocretin-1/orexin-A (Hcrt-1/Ox-A) immunoreactive neurons in the lateral hypothalamus (LH) and in the perifornical area (PeF). Of note, intra-LH and intra-PeF administration of NPS increased conditioned reinstatement of cocaine responding, an effect that was selectively blocked with the Hcrt-1/Ox-A receptor selective antagonist SB334867. Finally, results showed that intra-LH injection of the NPS antagonist [D-Cys(tBu) (5)]NPS blocked cue-induced cocaine seeking, indicating a role for this system in the pathophysiology of drug relapse. I n 2004, Xu and colleagues reported the deorphanization of a novel neuropeptide system named neuropeptide S (NPS). NPS binds to Gs and Gq protein-coupled receptors, previously identified as GPR154 and now referred to as NPSR (1, 2). NPS peptide transcript is expressed predominantly in a small group of neurons located between the locus ceruleus (LC), the Barrington nucleus, and the parabrachial nuclei. NPSR mRNA is expressed throughout the central nervous system with the highest concentration in olfactory structures, the amygdaloid complex, the paraventricular thalamic nucleus, the subiculum, and the lateral (LH), dorsomedial (DMH), and ventromedial hypothalamus (VMH) (1, 2).Activation of NPSR by intracerebroventricular (ICV) injection of NPS stimulates locomotor activity, increases arousal, and suppresses all stages of sleep (1). ICV injection of NPS has also been shown to stimulate hypothalamic-pituitary-adrenal axis (HPA) activity, enhancing plasma adrenocorticotropic hormone (ACTH) and corticosterone levels (3). Our group has recently found that intra-LH injections of NPS facilitate conditioned reinstatement of alcohol seeking (4). In addition, Pañeda and coworkers reported that ICV NPS itself increased relapse-like behavior in mice previously trained to self-administer cocaine. This latter effect was blocked by CRF1R antagonism and was absent in CRF1 receptor KO mice, suggesting a stress-like effect of NPS under the experimental conditions used (5).In the present study, to examine further the significance of the NPS system in the pathophysiology of cocaine craving and relapse, we studied the effect of NPS and of the selective NPSR antagonists SHA 68 (3-oxo-1,1-diphenyl-tetrahydro-oxazolo[3,4-a]pyrazine-7-ca...

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“…In this respect, it would be highly interesting to study the possible link between the NPS system, alcohol withdrawal, and anxiety, given that on the one hand, NPS is related to anxiolytic-like actions (Leonard et al, 2008;Vitale et al, 2008), while on the other, it activates the HPA axis and increases relapse to alcohol and cocaine seeking elicited by conditioning factors and drug priming, respectively (Cannella et al, 2009;Paneda et al, 2009). …”

Section: N Europeptide S (Nps) Is the Recently Discovered Endogenousmentioning

confidence: 99%

Neuropeptide S Receptor Gene Expression in Alcohol Withdrawal and Protracted Abstinence in Postdependent Rats

Ruggeri

Braconi

Cannella

et al. 2009

Alcoholism Clin & Exp Res

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Background: Alcoholism is a chronic disease characterized by frequent intoxications followed by withdrawal episodes and relapse to alcohol use. Neuroplastic changes associated with these intoxication and withdrawal cycles are thought to play a key role in disease progression. Recently, it has been shown that neuropeptide S (NPS), a newly deorphanized neuropeptide receptor system, facilitates relapse to alcohol seeking in laboratory animals. Given that a history of ethanol intoxication may increase vulnerability to alcohol addiction, we sought to determine whether NPS receptor (NPSR) gene expression is altered during withdrawal.Methods: Rats were subjected to 1 week of intoxication by oral alcohol administration. NPSR gene expression was analyzed by in situ hybridization in rats 12 hours and 7 days after the last alcohol administration. To investigate the functional significance of NPSR system adaptation following protracted withdrawal 7 days after intoxication, we tested the anxiolytic-like properties of NPS in nondependent and postdependent rats using the shock probe defensive burying test (DB).Results: At both time points, increased NPSR gene expression was observed in several brain areas, including the endopiriform nucleus, the motor cortex, and the medial amygdaloid nucleus. Moderate increases in gene expression were also found in the lateral hypothalamus, paraventricular nucleus, basolateral and central amygdala. Differences from control animals were more pronounced after 7 days of abstinence. The upregulation of the NPSR system at this time point was confirmed by functional data indicating that intracerebroventricular (ICV) NPS administration (0.0, 0.3, and 0.1 nmol ⁄ rat) elicits more pronounced anxiolytic effects in postdependent animals than in controls subjected to the electric shock probe DB test.Conclusions: Neuropeptide S receptor mRNA expression is increased in different brain areas of postdependent rats; as shown in the DB test, this expression change is functionally relevant.

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Neuropeptide S Reinstates Cocaine-Seeking Behavior and Increases Locomotor Activity through Corticotropin-Releasing Factor Receptor 1 in Mice

Cited by 96 publications

References 35 publications

A neuropeptide S receptor variant associated with overinterpretation of fear reactions: a potential neurogenetic basis for catastrophizing

A neuropeptide S receptor variant associated with overinterpretation of fear reactions: a potential neurogenetic basis for catastrophizing

Neuropeptide S facilitates cue-induced relapse to cocaine seeking through activation of the hypothalamic hypocretin system

Neuropeptide S Receptor Gene Expression in Alcohol Withdrawal and Protracted Abstinence in Postdependent Rats

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