Neuronal differentiation of mesenchymal stem cells by polyvinyl alcohol/Gelatin/crocin and beta-carotene

Asghari, Niloofar; Irani, Shiva; Pezeshki-Moddaress, Mohamad; Zandi, Mojgan; Mohamadali, Marjan

doi:10.1007/s11033-022-07123-8

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…More and more studies reported that since electrospinning fibers-based scaffolds required appropriate mechanical strength and topographical cures, it is common to select polymers from natural or synthetic sources to prepare composite scaffolds for neural tissue engineering. For example, Asghari and colleagues reported a composite nanofiber scaffold and explored its application in nerve tissue repair and regeneration [16]. They fabricated nanofibers with polyvinyl alcohol, gelatin and crocin by electrospinning technology, and investigated the effects of crocin concentration on proliferation and differentiation of mesenchymal stem cells (MSCs).…”

Section: Electrospinningmentioning

confidence: 99%

Recent progresses in neural tissue engineering using topographic scaffolds

Han

2024

Am J Stem Cells

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Neural tissue engineering as alternatives to recover damaged tissues and organs is getting more and more attention due to the lack of regeneration ability of natural tissue nervous system after injury. Particularly, topographic scaffolds are one of the critical elements to guide nerve orientation and reconnection with characteristics of mimic the natural extracellular matrix. This review focuses on scaffolds preparation technologies, topographical features, scaffolds-based encapsulations delivery strategies for neural tissue regeneration, biological functions on nerve cell guidance and regeneration, and applications of topographic scaffolds in vivo and in vitro. Here, the recent developments in topographic scaffolds for neural tissue engineering by simulating neural cell topographic orientation and differentiation are presented. We also explore the challenges and future perspectives of topographical scaffolds in clinical trials and practical applications.

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Section: Electrospinningmentioning

confidence: 99%

Recent progresses in neural tissue engineering using topographic scaffolds

Han

2024

Am J Stem Cells

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“…Therefore, βC increases neural differentiation by improving the phosphorylation of extracellular signal-regulated kinases (ERK) [34]. The ability of βC to induce neurogenesis for stem cells has been reported recently [30][31][32][33][34].…”

Section: Neural Differentiationmentioning

confidence: 99%

“…Because of conjugated double bonds, βC has electrical activity [28,29]. The ability of βC to induce neurogenesis for stem cells has been reported recently [30][31][32][33][34]. This research aimed to fabricate electroconductive nanofibers based on PANi/PAN via the electrospinning process and then surface-functionalization with CMC.…”

Section: Introductionmentioning

confidence: 99%

Carboxymethyl Chitosan-Functionalized Polyaniline/Polyacrylonitrile Nano-Fibers for Neural Differentiation of Mesenchymal Stem Cells

Solimani

Irani

Mohamadali

et al. 2023

Appl Biochem Biotechnol

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Electroconductive scaffolds based on polyaniline (PANi)/polyacrylonitrile (PAN) were fabricated and surface-functionalized by carboxymethyl chitosan (CMC) as efficient scaffolds for nerve tissue regeneration. The results of scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, and water contact angle measurement approved the successful fabrication of CMC-functionalized PANi/PAN-based scaffolds. Human adipose-derived mesenchymal stem cells (hADMSCs) were cultured on the scaffolds for 10 d in the presence or absence of β-carotene (βC, 20 µM) as a natural neural differentiation agent. The MTT and SEM results confirmed the attachment and proliferation of hADMSCs on the scaffolds. The expression of MAP2 at the mRNA and protein levels showed the synergic neurogenic induction effect of CMC-functionalization and βC for hADMSCs on the scaffolds. The CMC-functionalized nanofibrous PANi/PAN-based scaffolds are potential candidates for nerve tissue engineering.

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Effects of crocin on the enhancement of in vitro neurogenesis: Involvement of Notch and CREB/BDNF signaling pathways

Vafaei,

Mirzaie,

Baghalishahi

et al. 2023

Preprint

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Introduction: Adult neurogenesis, the process of generating new neuron cells in adult CNS, continues throughout life, despite past perceptions. Unfortunately, this process is insufficient in pathological conditions and needs to be promoted by nutritional and pharmacological stimulants. Crocin, the active component of Saffron, is a carotenoid that affects neurogenesis in vivo and in vitro. Our aim in the present study was to investigate the enhancing effects of crocin on the neurogenesis of adipose-derived mesenchymal stem cells (ADSC) in the presence of retinoic acid, as well as the molecular pathways involved. Material and methods: Stemness potential and differentiation capacity of harvested ADSC cells were evaluated. The optimum dose of crocin was assessed with an MTT assay. Crocin, retinoic acid, CREB/BDNF, and Notch inhibitors alone and in combination were added to the cell culture medium. Jag1, Hes1, Notch, and BDNF gene expression were analyzed by q-RTPCR on days 7, 14, and 21, while CREB, DCX, SOX2, and NeuN expression in the different groups were analyzed by Immunofluorescence (IF) method. Results: Expression of mesenchymal CD markers as well as adipogenic and osteogenic differentiation confirmed the origin and properties of ADSCs. The optimal dose of crocin for in-vitro use was 1mM. Administration of crocin significantly (P<0.05) increased, while administration of inhibitors (DATP & Naphthol) significantly (P<0.05) decreased in Jag1, Hes1, Notch, and BDNF expression. Immunofluorescent assessments showed that expression of DCX, BDNF, NeuN, and Sox2 proteins increased significantly (P<0.05) after crocin administration and decreased significantly (P<0.05) after administration of the inhibitor. Conclusion: It can be concluded that crocin can be used as an enhancer for neural differentiation of MSCs in-vitro in the presence of retinoic acid. The mechanism is proposed through Notch and CREB/BDNF signaling pathways. Whether these effects can occur in vivo requires more extensive studies.

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Neuronal differentiation of mesenchymal stem cells by polyvinyl alcohol/Gelatin/crocin and beta-carotene

Cited by 4 publications

References 33 publications

Recent progresses in neural tissue engineering using topographic scaffolds

Recent progresses in neural tissue engineering using topographic scaffolds

Carboxymethyl Chitosan-Functionalized Polyaniline/Polyacrylonitrile Nano-Fibers for Neural Differentiation of Mesenchymal Stem Cells

Effects of crocin on the enhancement of in vitro neurogenesis: Involvement of Notch and CREB/BDNF signaling pathways

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