Neuromuscular physiology of giant muscle fibers of a barnacle, Balanus nubilus Darwin

Hoyle, Graham; Smyth, Thomas

doi:10.1016/0010-406x(63)90229-9

Cited by 136 publications

(65 citation statements)

References 6 publications

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“…The figures in the first column give concentrations per kilo wet weight while those in the second column show concentrations per liter of internal water estimated from the observed water content of the fiber (76.7 -0.5 per cent of the wet weight). These values of the I66 internal K+ concentration are smaller than those found for the squid giant axon (Steinbach and Spiegelman, 1943;Keynes and Lewis, 1951) but show a good agreement with those found for some marine crustacean muscle fibers (Shaw, 1955). Since the internal Na concentration is small the major internal cations are probably organic as in the case of crab muscle fiber (Shaw, 1955).…”

Section: Internal Ionic Composition Of the Normal Fibersupporting

confidence: 85%

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The Effects of Various Ions on Resting and Spike Potentials of Barnacle Muscle Fibers

Hagiwara

Chichibu

Naka

1964

The Journal of General Physiology

134

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Effects of monovalent cations and some anions on the electrical properties of the barnacle muscle fiber membrane were studied when the intraor extracellular concentrations of those ions were altered by longitudinal intracellular injection. The resting potential of the normal fiber decreases linearly with increase of logarithm of [K+] the internal K+ concentration is decreased the overshoot of the spike potential increases and the time course of the spike potential becomes more prolonged. In substituting for the internal K+, Na+ and sucrose affect the resting and spike potentials similarly. Some organic cations (guanidine, choline, tris, and TMA) behave like sucrose while some other organic cations (TEA, TPA, and TBA) have a specific effect and prolong the spike potential if they are applied intracellularly or extracellularly. In all cases the active membrane potential increases linearly with the logarithm of [Ca++]out/[K+]i n and the increment is about 29 mv for tenfold increase in this ratio. The fiber membrane is permeable to C1-and other smaller anions (Br-and I-) but not to acetate-and larger anions (citrate, sulfate-, and methanesulfonate-).

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Section: Internal Ionic Composition Of the Normal Fibersupporting

confidence: 85%

“…Recently giant muscle fibers with diameters of 0.5 to 2.5 mm have been described in the barnacle, Balanus nubilus by Hoyle and Smyth (1963 a, b). Because of the large size the internal ionic composition can be altered by intracellular injection.…”

Section: The Journal Of General Physiologymentioning

confidence: 99%

The Effects of Various Ions on Resting and Spike Potentials of Barnacle Muscle Fibers

Hagiwara

Chichibu

Naka

1964

The Journal of General Physiology

134

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“…Preparation of the muscle bundles was described by Hoyle & Smyth (1963). The depressor 8cutorum rostralts were used most frequently because these fibres presented a more circular cross-section.…”

Section: Methodsmentioning

confidence: 99%

Effect of temperature on membrane potential and ionic fluxes in intact and dialysed barnacle muscle fibres

DiPolo¹,

Latorre²

1972

The Journal of Physiology

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SUMMARY1. The temperature-dependent component of the resting potential in intact, cannulated and dialysed fibres from the muscle of the barnacle Balanus nubilus was studied under a variety of different experimental conditions. A decrease in temperature from 22 to 120 C produced a mean depolarization of 10 mV.2. Neither addition of strophanthidin, nor replacement of external sodium by lithium affect the voltage shift induced by temperature. However, the magnitude of the voltage shift depends on the external chloride and potassium concentration.3. The dialysis technique was applied to measure the potassium, chloride and sodium fluxes as a function of temperature. The QL0 for the passive fluxes of these ions was 1-9, 1P7, and 1P4 respectively.4. The temperature-dependent changes in the passive ionic fluxes combined with the inability of inhibitors of the sodium pump to alter the temperature dependence of the resting potential suggest that the change induced by temperature on the resting potential is primarily caused by a change in the passive permeability ratios, and is not related to active ion transport.

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“…The myofibrils were prepared from single muscle fibres isolated from the lateral depressor muscles of the acorn barnacle Balanus nubilus Darwin (Hoyle & Smyth, 1963). The single muscle fibre was blotted to remove excess saline and placed on the bottom of a small Perspex cell.…”

Section: Dis8ectionmentioning

confidence: 99%

Effect of changing the composition of the bathing solution upon the isometric tension—pCa relationship in bundles of crustacean myofibrils

Ashley¹,

Moisescu²

1977

The Journal of Physiology

170

123

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SUMMARY1. The relative isometric tension-pCa relationship has been determined for isolated bundles of barnacle myofibrils under a variety of ionic conditions using [Ca2+]-buffered solutions which also contained an ATP regenerating system (creatine phosphate and creatine kinase).2. The results are in better agreement with the 'consecutive' scheme of reaction rather than with the 'independent' alternative (Ashley & Moisescu, 1972) for the co-operative action of two Ca2+ ions in the process of tension activation in crustacean skeletal muscle.3. Variations in the pH of the activating solutions did have a marked effect on the relative tension-pCa curve, although no effect was observed on the absolute maximum value for isometric tension. A shift in pH by 0-5 u. in the range 6-6-7-6 shifted the Ca2+-activation curve by 0.5 log u. towards lower free Ca2+ concentrations.4. Changes in the free Mg2+ concentration of the activating solutions in the millimolar range produced a pronounced shift of the relative tension-pCa curve along the pCa axis. Increasing [Mg2+] from 1 to 5 mM shifted the curve by about 0-7 log u. to higher free Ca2+ concentrations, without significantly modifying its steepness.5. Changes in the MgATP concentration of the activating solutions in the range of 1-13 mm had no significant effect on the relative tension-pCa relationship.6. Varying the K+ concentration in the activating solutions was also

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Neuromuscular physiology of giant muscle fibers of a barnacle, Balanus nubilus Darwin

Cited by 136 publications

References 6 publications

The Effects of Various Ions on Resting and Spike Potentials of Barnacle Muscle Fibers

The Effects of Various Ions on Resting and Spike Potentials of Barnacle Muscle Fibers

Effect of temperature on membrane potential and ionic fluxes in intact and dialysed barnacle muscle fibres

Effect of changing the composition of the bathing solution upon the isometric tension—pCa relationship in bundles of crustacean myofibrils

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