NeuroD1 overexpression in spinal neurons accelerates axonal regeneration after sciatic nerve injury

Lai, Muhua; Pan, Mengjie; Ge, Longjiao; Liu, Jingmin; Deng, Junyao; Wang, Xianghai; Li, Lixia; Wen, Jinkun; Tan, Dandan; Zhang, Haowen; Hu, Xiaofang; Fu, Lanya; Xu, Yizhou; Li, Zhenlin; Qiu, Xiaozhong; Chen, Gong; Guo, Jiasong

doi:10.1016/j.expneurol.2020.113215

Cited by 15 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…investigation. NeuroD1 overexpression has shown promises in regenerative therapy following spinal cord injury and sciatic nerve injuries (Puls et al, 2020;Lai et al, 2020). Given that NeuroD1 partners with other bHLH TFs, discovering such partner TFs may increase success in efforts towards regenerative medicine.…”

Section: Discussionmentioning

confidence: 99%

“…Many of these TFs are transcriptional repressors or activators, inhibitors of bHLH dimer formation as well as a component of chromatin remodelling complexes; hence, they are interesting candidates for future investigation. NeuroD1 overexpression has shown promises in regenerative therapy following spinal cord injury and sciatic nerve injuries ( Puls et al, 2020 ; Lai et al, 2020 ). Given that NeuroD1 partners with other bHLH TFs, discovering such partner TFs may increase success in efforts towards regenerative medicine.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tcf12 and NeuroD1 cooperatively drive neuronal migration during cortical development

et al. 2022

View full text Add to dashboard Cite

Corticogenesis consists of a series of synchronised events, including fate transition of cortical progenitors, neuronal migration, specification and connectivity. NeuroD1, a basic helix-loop-helix (bHLH) transcription factor (TF), contributes to all of these events, but how it coordinates these independently is still unknown. Here, we demonstrate that NeuroD1 expression is accompanied by a gain of active chromatin at a large number of genomic loci. Interestingly, transcriptional activation of these loci relied on a high local density of adjacent bHLH TFs motifs, including, predominantly, Tcf12. We found that activity and expression levels of Tcf12 were high in cells with induced levels of NeuroD1 that spanned the transition of cortical progenitors from proliferative to neurogenic divisions. Moreover, Tcf12 forms a complex with NeuroD1 and co-occupies a subset of NeuroD1 target loci. This Tcf12-NeuroD1 cooperativity is essential for gaining active chromatin and targeted expression of genes involved in cell migration. By functional manipulation in vivo, we further show that Tcf12 is essential during cortical development for the correct migration of newborn neurons and, hence, for proper cortical lamination.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tcf12 and NeuroD1 cooperatively drive neuronal migration during cortical development

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Since AAV9 transduces both the neuronal and glial cells, utilizing neuron-specific synapsin promoter in the viral vector is necessary. Synapsin promoter driven AAV9 viral vectors have been applied by intravenous, intracerebroventricular, and spinal cord injections [32,44,45]. In this study, the viral vectors AAV9.hSyn.YFP-IMPs were inoculated in the injury-conditioned DRG culture.…”

Section: Discussionmentioning

confidence: 99%

Neuron-Specific IMP2 Overexpression by Synapsin Promoter-Driven AAV9: A Tool to Study Its Role in Axon Regeneration

Blizard

Park

O'Toole

et al. 2021

Cells

View full text Add to dashboard Cite

Insulin-like growth factor II mRNA-binding protein (IMP) 2 is one of the three homologues (IMP1-3) that belong to a conserved family of mRNA-binding proteins. Its alternative splice product is aberrantly expressed in human hepatocellular carcinoma, and it is therefore identified as HCC. Previous works have indicated that IMP1/ZBP1 (zipcode binding protein) is critical in axon guidance and regeneration by regulating localization and translation of specific mRNAs. However, the role of IMP2 in the nervous system is largely unknown. We used the synapsin promoter-driven adeno-associated viral (AAV) 9 constructs for transgene expression both in vitro and in vivo. These viral vectors have proven to be effective to transduce the neuron-specific overexpression of IMP2 and HCC. Applying this viral vector in the injury-conditioned dorsal root ganglion (DRG) culture demonstrates that overexpression of IMP2 significantly inhibits axons regenerating from the neurons, whereas overexpression of HCC barely interrupts the process. Quantitative analysis of binding affinities of IMPs to β-actin mRNA reveals that it is closely associated with their roles in axon regeneration. Although IMPs share significant structural homology, the distinctive functions imply their different ability to localize specific mRNAs and to regulate the axonal translation.

show abstract

“…Six hours after the final behavioral test, the rats were anesthetized with 180 mg /kg tribromoethanol, and electrophysiological testing was performed (Pan et al, 2017;Li et al, 2019;Hu et al, 2020;Lai et al, 2020). Briefly, a pair of needle electrodes was inserted to stimulate the sciatic nerve 3 mm proximal to the crush site, and a pair of recording electrodes was inserted subcutaneously into the middle of the intrinsic foot muscle.…”

Section: Behavioral Assessment and Electrophysiological Testingmentioning

confidence: 99%

“…To assess the role of microtubule dynamics on nerve regeneration, rats were subjected to sciatic nerve crush injury and immediately treated with paclitaxel, nocodazole, or saline (vehicle). Three days later, immunohistochemistry for GAP43 (a marker of axonal regeneration (Romeo-Guitart et al, 2020)) or SCG10 (a marker for regenerating sensory axons (Lai et al, 2020)) was performed on the injured nerves to detect regenerating axons. On cross sections taken 5 mm distal to the injured site, we found the highest density of both GAP43-and SCG10-positive axons in the paclitaxel group and the lowest density in the nocodazole group (P < 0.05; Figure 6A-D).…”

Section: Effects Of Paclitaxel and Nocodazole On Nerve Regeneration After Sciatic Nerve Crush Injurymentioning

confidence: 99%

Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury

Liu

Zou

et al. 2022

Neural Regen Res

Self Cite

View full text Add to dashboard Cite

NeuroD1 overexpression in spinal neurons accelerates axonal regeneration after sciatic nerve injury

Cited by 15 publications

References 42 publications

Tcf12 and NeuroD1 cooperatively drive neuronal migration during cortical development

Tcf12 and NeuroD1 cooperatively drive neuronal migration during cortical development

Neuron-Specific IMP2 Overexpression by Synapsin Promoter-Driven AAV9: A Tool to Study Its Role in Axon Regeneration

Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury

Contact Info

Product

Resources

About