Neural Stem/Progenitor Cells of Human Olfactory Mucosa for the Treatment of Chronic Spinal Cord Injuries

Воронова, А Д; Степанова, О. В.; Valikhov, Marat P.; Чадин, А. В.; Semkina, Alevtina S.; Чехонин, В. П.

doi:10.1007/s10517-020-04749-1

Cited by 12 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potential therapeutic benefits of OE-MSCs were also tested in animal models of various injuries/pathologies affecting the peripheral or central nervous system. Promising results have been reported in models replicating some of the symptoms/mechanisms observed in Alzheimer’s disease ( Hong et al, 2022 ), Parkinson’s disease ( Murrell et al, 2008 ; Farhadi et al, 2021 ; Simorgh et al, 2021 ), Huntington’s disease ( Bayat et al, 2021 ), multiple sclerosis ( Lindsay et al, 2010 , 2022 ; Wu et al, 2013 ), cerebellar ataxia ( Moghaddam et al, 2022 ) ischemic stroke ( Liu et al, 2020 ; Zhuo et al, 2021 ), spinal cord injury ( Lindsay et al, 2017 ; Stamegna et al, 2018 ; Voronova et al, 2020 ; Hamidabadi et al, 2021 ), amnesia ( Nivet et al, 2011 ), hearing loss ( Pandit et al, 2011 ). In regard to mechanisms, it has been reported that OE-MSCs secrete trophic factors, modulate the inflammatory response, phagocyte inclusions or deleterious molecules, regulate oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Human nasal olfactory stem cells, purified as advanced therapy medicinal products, improve neuronal differentiation

et al. 2022

View full text Add to dashboard Cite

BackgroundOlfactory ecto-mesenchymal stem cells (OE-MSC) are mesenchymal stem cells derived from the lamina propria of the nasal mucosa. They display neurogenic and immunomodulatory properties and were shown to induce recovery in animal models of spinal cord trauma, hearing loss, Parkinsons’s disease, amnesia, and peripheral nerve injury. As a step toward clinical practice, we sought to (i) devise a culture protocol that meets the requirements set by human health agencies and (ii) assess the efficacy of stem cells on neuron differentiation.MethodsNasal olfactory mucosa biopsies from three donors were used to design and validate the good manufacturing process for purifying stem cells. All processes and procedures were performed by expert staff from the cell therapy laboratory of the public hospital of Marseille (AP-HM), according to aseptic handling manipulations. Premises, materials and air were kept clean at all times to avoid cross-contamination, accidents, or even fatalities. Purified stem cells were cultivated for 24 or 48 h and conditioned media were collected before being added to the culture medium of the neuroblastoma cell line Neuro2a.ResultsCompared to the explant culture-based protocol, enzymatic digestion provides higher cell numbers more rapidly and is less prone to contamination. The use of platelet lysate in place of fetal calf serum is effective in promoting higher cell proliferation (the percentage of CFU-F progenitors is 15.5%), with the optimal percentage of platelet lysate being 10%. Cultured OE-MSCs do not show chromosomal rearrangement and, as expected, express the usual phenotypic markers of mesenchymal stem cells. When incorporated in standard culture medium, the conditioned medium of purified OE-MSCs promotes cell differentiation of Neuro2a neuroblastoma cells.ConclusionWe developed a safer and more efficient manufacturing process for clinical grade olfactory stem cells. With this protocol, human OE-MSCs will soon be used in a Phase I clinical based on their autologous transplantation in digital nerves with a neglected injury. However, further studies are required to unveil the underlying mechanisms of action.

show abstract

Section: Discussionmentioning

confidence: 99%

Human nasal olfactory stem cells, purified as advanced therapy medicinal products, improve neuronal differentiation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…NSCs from olfactory mucosa have been the subject of special attention because of their potential to avoid graft-versus-host disease when transplanted autologously. As for extraction, in 2020 Voronova et al developed a protocol that can enrich NSCs from human olfactory mucosa, facilitating studies concerning transplantation therapies ( Voronova A. D. et al, 2020 ). Preliminary results showed that transplanting NSCs from human olfactory mucosa into recipient rats with chronic SCI may improve the motor activity of hind limbs.…”

Section: Sources Of Nscsmentioning

confidence: 99%

“…NSCs can be conveniently extracted from human participants instead of experimental animals. These sources of NSCs have proved their capabilities for significant, although limited, functional recovery in a rat contusion SCI model at T9–T10 level ( Voronova A. et al, 2020 ).…”

Section: Applications Of Nsc Transplantation Therapy To Treat Scimentioning

confidence: 99%

The roles and applications of neural stem cells in spinal cord injury repair

Guo

Zhang

Zhai

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Spinal cord injury (SCI), which has no current cure, places a severe burden on patients. Stem cell-based therapies are considered promising in attempts to repair injured spinal cords; such options include neural stem cells (NSCs). NSCs are multipotent stem cells that differentiate into neuronal and neuroglial lineages. This feature makes NSCs suitable candidates for regenerating injured spinal cords. Many studies have revealed the therapeutic potential of NSCs. In this review, we discuss from an integrated view how NSCs can help SCI repair. We will discuss the sources and therapeutic potential of NSCs, as well as representative pre-clinical studies and clinical trials of NSC-based therapies for SCI repair.

show abstract

“…Some researchers have attempted to transplant stem cells at the chronic stage. Wertheim et al removed the scar of the chronic stage and transplanted hydrogel-encapsulated iPSCs to the injured site, resulting in good functional recovery, and they also transplanted neural stem cells/progenitor cells from human olfactory epithelial mucosa, resulting in improved hind limb motor function of rats ( Voronova et al, 2020 ).…”

Section: Pathological Changes In the Immune Microenvironment In Spina...mentioning

confidence: 99%

The immune microenvironment and tissue engineering strategies for spinal cord regeneration

Yuan

Peng

Jie

et al. 2022

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Regeneration of neural tissue is limited following spinal cord injury (SCI). Successful regeneration of injured nerves requires the intrinsic regenerative capability of the neurons and a suitable microenvironment. However, the local microenvironment is damaged, including insufficient intraneural vascularization, prolonged immune responses, overactive immune responses, dysregulated bioenergetic metabolism and terminated bioelectrical conduction. Among them, the immune microenvironment formed by immune cells and cytokines plays a dual role in inflammation and regeneration. Few studies have focused on the role of the immune microenvironment in spinal cord regeneration. Here, we summarize those findings involving various immune cells (neutrophils, monocytes, microglia and T lymphocytes) after SCI. The pathological changes that occur in the local microenvironment and the function of immune cells are described. We also summarize and discuss the current strategies for treating SCI with tissue-engineered biomaterials from the perspective of the immune microenvironment.

show abstract

Neural Stem/Progenitor Cells of Human Olfactory Mucosa for the Treatment of Chronic Spinal Cord Injuries

Cited by 12 publications

References 15 publications

Human nasal olfactory stem cells, purified as advanced therapy medicinal products, improve neuronal differentiation

Human nasal olfactory stem cells, purified as advanced therapy medicinal products, improve neuronal differentiation

The roles and applications of neural stem cells in spinal cord injury repair

The immune microenvironment and tissue engineering strategies for spinal cord regeneration

Contact Info

Product

Resources

About