Neural precursors express multiple chondroitin sulfate proteoglycans, including the lectican family

Kabos, Peter; Matundan, Harry H.; Zandian, Mandana; Bertolotto, Corine; Robinson, Michael L.; Davy, Brian E.; Yu, John S.; Krueger, Richard C.

doi:10.1016/j.bbrc.2004.04.114

Cited by 56 publications

(53 citation statements)

References 44 publications

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“…1B) A unique CS-DS structure containing CS-A, CS-D and DS patterns is recognized by the monoclonal antibody 473HD (Clement et al, 1998;Ito et al, 2005) as a marker for radial glial cells (Kabos et al, 2004). To investigate the influence of Chst11 or Chst14 depletion on the expression of CS-DS structures in neurospheres, western blot analysis was performed using the 473HD antibody on protein extracts of neurospheres originating from the embryonic forebrain of Chst11 2/2 and Chst14 2/2 mice in comparison with their wild-type littermates.…”

Section: Resultsmentioning

confidence: 99%

“…Chondroitin sulfate (CS) and dermatan sulfate (DS) polysaccharide chains are important glycosaminoglycans (GAGs) of the extracellular matrix (ECM). They are composed of the alternating disaccharides Nacetylgalactosamine (GalNAc) and glucuronic acid (GlcA) or iduronic acid (IdoA) (Bulow and Hobert, 2006), which can be sulfated at different positions and have been shown to be present in the neurogenic regions of the embryonic and adult central nervous system (Ida et al, 2006;Kabos et al, 2004;Sugahara and Mikami, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells

Bian

Akyüz

Bernreuther

et al. 2011

Journal of Cell Science

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SummaryChondroitin sulfates (CSs) and dermatan sulfates (DSs) are enriched in the microenvironment of neural stem cells (NSCs) during development and in the adult neurogenic niche, and have been implicated in mechanisms governing neural precursor migration, proliferation and differentiation. In contrast to previous studies, in which a chondroitinaseABC-dependent unselective deglycosylation of both CSs and DSs was performed, we used chondroitin 4-O-sulfotransferase-1 (Chst11/C4st1)-and dermatan 4-O-sulfotransferase-1 (Chst14/D4st1)-deficient NSCs specific for CSs and DSs, respectively, to investigate the involvement of specific sulfation profiles of CS and DS chains, and thus the potentially distinct roles of CSs and DSs in NSC biology. In comparison to wild-type controls, deficiency for Chst14 resulted in decreased neurogenesis and diminished proliferation of NSCs accompanied by increased expression of GLAST and decreased expression of Mash-1, and an upregulation of the expression of the receptors for fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). By contrast, deficiency in Chst11 did not influence NSC proliferation, migration or differentiation. These observations indicate for the first time that CSs and DSs play distinct roles in the self-renewal and differentiation of NSCs.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells

Bian

Akyüz

Bernreuther

et al. 2011

Journal of Cell Science

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“…2 and 3). Recently, Kabos et al (14) reported that aggrecan proteins as well as phosphacan protein was detected in the culture medium of neurospheres. These results suggest that neural stem/progenitor cells participate in the construction of their own milieu by synthesizing these CSPGs and depositing them in their surroundings.…”

Section: Table 2 Disaccharide Composition Of Hs In Gag Fractions Prepmentioning

confidence: 99%

“…However, very few studies have been done on the structure and function of CS-GAG/CSPGs in the brain primordium. Recently, neural precursor cells have been shown to synthesize and secrete CSPGs, including some lectican family members (14). In addition, it has been reported that cultured tissue segments of E13 mouse brains synthesize CS rich in a disaccharide unit containing 4,6-disulfated N-acetylgalactosamine (E-unit) and that the CS preparation has an affinity for midkine, a heparin-binding growth factor (15).…”

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confidence: 99%

Identification and Functions of Chondroitin Sulfate in the Milieu of Neural Stem Cells

Ida

Shuo

Hirano

et al. 2006

Journal of Biological Chemistry

125

105

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The behavior of cells is generally considered to be regulated by environmental factors, but the molecules in the milieu of neural stem cells have been little studied. We found by immunohistochemistry that chondroitin sulfate (CS) existed in the surroundings of nestin-positive cells or neural stem/progenitor cells in the rat ventricular zone of the telencephalon at embryonic day 14. Brain-specific chondroitin sulfate proteoglycans (CSPGs), including neurocan, phosphacan/receptor-type protein-tyrosine phosphatase ␤, and neuroglycan C, were detected in the ventricular zone. Neurospheres formed by cells from the fetal telencephalon also expressed these CSPGs and NG2 proteoglycan. To examine the structural features and functions of CS polysaccharides in the milieu of neural stem cells, we isolated and purified CS from embryonic day 14 telencephalons. The CS preparation consisted of two fractions differing in size and extent of sulfation: small CS polysaccharides with low sulfation and large CS polysaccharides with high sulfation. Interestingly, both CS polysaccharides and commercial preparations of dermatan sulfate CS-B and an E-type of highly sulfated CS promoted the fibroblast growth factor-2-mediated proliferation of neural stem/progenitor cells. None of these CS preparations promoted the epidermal growth factor-mediated neural stem cell proliferation. These results suggest that these CSPGs are involved in the proliferation of neural stem cells as a group of cell microenvironmental factors.

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“…NSPCs by themselves participate in the construction of their own milieu by synthesizing CS-PGs, including lectican PG family members (aggrecan, versican, neurocan, and brevican) (76), and depositing them in their surroundings. Consistent with these observations, several CS-PGs were detected in neurospheres, which are cellular aggregates that grow in suspension and are composed of NSPCs and differentiating progeny.…”

Section: Cs/ds-pgs Expressing Functional "Wobble Oligosaccharide Motimentioning

confidence: 99%

Chondroitin Sulfate “Wobble Motifs” Modulate Maintenance and Differentiation of Neural Stem Cells and Their Progeny

Purushothaman

Sugahara

Faissner

2012

Journal of Biological Chemistry

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Chondroitin sulfate/dermatan sulfate (CS/DS) proteoglycans, major components of the central nervous system, have the potential to interact with a wide range of growth factors and neurotrophic factors that influence neuronal migration, axon guidance pathways, and neurite outgrowth. Recent studies have also revealed the role of CS/DS chains in the orchestration of the neural stem/progenitor cell micromilieu. Individual functional proteins recognize a set of multiple overlapping oligosaccharide sequences decorated to give different sulfation patterns, which are termed here "wobble CS/DS oligosaccharide motifs," and induce signaling pathways essential for the proliferation, selfrenewal, and cell lineage commitment of neural stem/progenitor cells.The discovery of populations of multipotent self-renewing neural stem cells within fetal and adult brains has raised the hope of developing new therapeutic strategies for CNS disorders. Neural stem/progenitor cells (NSPCs) 3 are defined as self-renewing multipotential cells that can generate all types of neural cells, including neurons and glia (astrocytes and oligodendrocytes). In an adult brain, NSPCs are present in two distinct regions: in the subgranular zone of the dentate gyrus of the hippocampus and in the subventricular zone (SVZ) of the lateral ventricles (1-4). During brain development, the neuroepithelial cells of the neural tube expand and self-renew by symmetric division. With increasing thickness of the neuroectoderm, radial glial cells emerge and fulfill the role of neural stem cells. In the first wave, these cells self-renew by symmetrical divisions. In parallel, an asymmetric division pattern develops in which each division cycle gives rise to a radial glial cell and a neuronal progenitor. This phase of neurogenesis is followed by a phase of gliogenesis. In many regions of the CNS, oligodendrocytes precede the formation of astrocytes, which constitute the final population that is formed in the developing CNS (5). The radial glial cells can transform into astrocytes, and the subpopulation of astrocytes in the SVZ has been identified as NSPCs in the adult brain (6). Thereafter, the radial glia recede. Adult forms of radial glia are preserved as Bergmann glia and Müller glia solely in the cerebellum and retina, respectively (7, 8). Thus, NSPCs, which are characterized by their high proliferative potential while retaining self-renewal and pluripotency, encompass neuroepithelial cells, radial glial cells, and SVZ astrocytes (9, 10). The self-renewal and differentiation properties of NSPCs are modulated by intrinsic factors such as transcription factors, intercellular interactions, and extrinsic factors present in the extracellular matrix (ECM). Understanding how these factors regulate the differentiation of NSPCs is essential to exploit potential therapeutic applications to treating various neurodegenerative disorders and spinal cord injuries. Neural Stem Cell NicheThe microenvironment where the NSPCs reside and maintain their self-renewal, proliferation, and d...

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Neural precursors express multiple chondroitin sulfate proteoglycans, including the lectican family

Cited by 56 publications

References 44 publications

Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells

Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells

Identification and Functions of Chondroitin Sulfate in the Milieu of Neural Stem Cells

Chondroitin Sulfate “Wobble Motifs” Modulate Maintenance and Differentiation of Neural Stem Cells and Their Progeny

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