Neural Differentiation of Mesenchymal Stem Cells on Scaffolds for Nerve Tissue Engineering Applications

Quintiliano, Kerlin; Crestani, Thayane Antoniolli; Silveira, Davi; Helfer, Virgínia Etges; Rosa, André Ricardo Pereira da; Balbueno, Eduardo Álvares; Steffens, Daniela; Jotz, Geraldo Pereira; Pilger, Diogo André; Pranke, Patrícia

doi:10.1089/cell.2016.0024

Cited by 11 publications

(3 citation statements)

References 36 publications

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“…Scaffold generation is the next strategy to consider in order to increase the neural capacities of MSCs. The scaffold structure resembles the ECM environment as it mimics the cellular niche [115], which could enhance the potential differentiation of MSCs into neuroglial cells in vivo. The scaffold helps to graft cells directly to the injured area where cells could regenerate injured structures [116,117].…”

Section: D Neural Scaffold Of Mscsmentioning

confidence: 99%

Interaction of Neural Stem Cells (NSCs) and Mesenchymal Stem Cells (MSCs) as a Promising Approach in Brain Study and Nerve Regeneration

Kamińska

Radoszkiewicz

Rybkowska

et al. 2022

Cells

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Rapid developments in stem cell research in recent years have provided a solid foundation for their use in medicine. Over the last few years, hundreds of clinical trials have been initiated in a wide panel of indications. Disorders and injuries of the nervous system still remain a challenge for the regenerative medicine. Neural stem cells (NSCs) are the optimal cells for the central nervous system restoration as they can differentiate into mature cells and, most importantly, functional neurons and glial cells. However, their application is limited by multiple factors such as difficult access to source material, limited cells number, problematic, long and expensive cultivation in vitro, and ethical considerations. On the other hand, according to the available clinical databases, most of the registered clinical trials involving cell therapies were carried out with the use of mesenchymal stem/stromal/signalling cells (MSCs) obtained from afterbirth or adult human somatic tissues. MSCs are the multipotent cells which can also differentiate into neuron-like and glia-like cells under proper conditions in vitro; however, their main therapeutic effect is more associated with secretory and supportive properties. MSCs, as a natural component of cell niche, affect the environment through immunomodulation as well as through the secretion of the trophic factors. In this review, we discuss various therapeutic strategies and activated mechanisms related to bilateral MSC–NSC interactions, differentiation of MSCs towards the neural cells (subpopulation of crest-derived cells) under the environmental conditions, bioscaffolds, or co-culture with NSCs by recreating the conditions of the neural cell niche.

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Section: D Neural Scaffold Of Mscsmentioning

confidence: 99%

Interaction of Neural Stem Cells (NSCs) and Mesenchymal Stem Cells (MSCs) as a Promising Approach in Brain Study and Nerve Regeneration

Kamińska

Radoszkiewicz

Rybkowska

et al. 2022

Cells

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show abstract

“…Moreover, the protein-loaded scaffolds successfully promoted the adhesion, proliferation and neurite extension of PC12 cells (a rat adrenal phaeochromocytoma cell line widely used as a neuronal cell model, as their phenotype is similar to that of sympathetic ganglion neurons after NGF-mediated differentiation [113]). A similar approach was used in another study, with the polymer matrix composed of PLGA [114].…”

Section: Growth Factor Deliverymentioning

confidence: 99%

Protein encapsulation by electrospinning and electrospraying

Moreira¹,

Lawson

Onyekuru

et al. 2021

Journal of Controlled Release

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“…Much research has been done on MSC differentiation into other lineages; MSCs have been proposed to differentiate into lineage of all three germ layers, such as: muscle, heart, liver, and neuronal, to name a few examples (the reader is referred to a number of critical reviews on this topic: [188][189][190] ). This pluripotent differentiation has been proposed to be mechanosensitive [20] and is being investigated in different 3D scaffolds [191,192] . Figure 3.…”

Section: Future Outlookmentioning

confidence: 99%

Introducing height to mechanobiology

Zonderland¹

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Mechanobiology, translating mechanical signals into biological ones, greatly affects cellular behavior. Steering cellular behavior for cell-based regenerative medicine approaches requires a thorough understanding of the orchestrating molecular mechanisms, among which mechanotransducive ones are being more and more elucidated. Differentiation and proliferation of the most widely used stem cells for tissue engineering applications, mesenchymal stromal cells, is highly mechanotransduction dependent. While the mechanotransduction pathways are relatively well-studied in 2D, much remains unknown of the role and regulation of these pathways in 3D. Ultimately, cells need to be cultured in a 3D environment to create functional de novo tissue. In this review, we explore the literature on the roles of different material properties on cellular behavior and mechanobiology in 2D and 3D. For example, while stiffness plays a dominant role in 2D MSC differentiation, it seems to be of subordinate importance in 3D MSC differentiation, where matrix remodeling seems to be key. Also, the role and regulation of some of the main mechanotransduction players are discussed, focusing on mesenchymal stromal cells. [20-23, 25-27, 29] >30 kPa Flat 2D hydrogels Non-remodelable 2D substrates 3D Huebsch et al. [46] 12-25 kPa Alginate-RGD, agarose-RGD or PEG-RGD Remodelable, non-covalent matrix bounds Khetan et al. [48] 4.4 kPa MMP-degrable hyaluronic acid hydrogels Remodelable, MMP degradable motives Ferreira et al. [50] 1 kPa Hyaluronic acid hydrogels Remodelable, degradability Chaudhuri et al. [61] 17 kPa Alginate-RGD hydrogels Remodelable, fast stress relaxation Darnell et al. [56] 18 kPa Alginate-RGD hydrogels Remodelable, fast stress relaxation Nam et al. [57] 15 kPa Alginate-RGD hydrogels Remodelable, fast stress relaxation Lee et al. [55] 20 kPa Alginate-RGD hydrogels Remodelable, fast stress relaxation No/Little osteogenesis 2D [20-23, 25-27, 29] <30 kPa Flat 2D hydrogels Non-remodelable 2D substrates 3D Huebsch et al. [46] >100 kPa Alginate-RGD, agarose-RGD or PEG-RGD Non-remodelable, too high stiffness Huebsch et al. [46] 2.5-5 kPa Alginate-RGD, agarose-RGD or PEG-RGD Remodelable, non-covalent matrix bounds Khetan et al. [48] 4-91 kPa Crosslinked hyaluronic acid hydrogels Non-remodelable, covalent matrix bounds Khetan et al. [48] 20 kPa Crosslinked alginate-RGD Non-remodelable, covalent matrix bounds Chaudhuri et al. [61] 17 kPa Alginate-RGD hydrogels Non-remodelable, slow stress relaxation Chaudhuri et al. [61] 9 kPa Alginate-RGD hydrogels Remodelable, fast stress relaxation Darnell et al. [56] 18 kPa Alginate-RGD hydrogels Non-remodelable, slow stress relaxation Nam et al. [57] 15 kPa Alginate-RGD hydrogels Non-remodelable, slow stress relaxation Lee et al. [55] 20 kPa Alginate-RGD hydrogels Non-remodelable, slow stress relaxation Adipogenesis 2D [21-27] 0.3-3 kPa Flat 2D hydrogels Non-remodelable 2D substrates 3D Huebsch et al. [46] 2.5-5 kPa Alginate-RGD, agarose-RGD or PEG-RGD Remodelable, non-covalent matrix bounds K...

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Neural Differentiation of Mesenchymal Stem Cells on Scaffolds for Nerve Tissue Engineering Applications

Cited by 11 publications

References 36 publications

Interaction of Neural Stem Cells (NSCs) and Mesenchymal Stem Cells (MSCs) as a Promising Approach in Brain Study and Nerve Regeneration

Interaction of Neural Stem Cells (NSCs) and Mesenchymal Stem Cells (MSCs) as a Promising Approach in Brain Study and Nerve Regeneration

Protein encapsulation by electrospinning and electrospraying

Introducing height to mechanobiology

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