Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions

Abstract

Many neural disorders are characterized by the loss of one or several types of neural cells. Human umbilical cord-derived mesenchymal cells (hUCMs) are capable of differentiating into neuron, astroglia-like and oligodendrocyte cell types. However, a reliable means of inducing the selective differentiation of hUCMs into neural cells in vitro has not yet been established. For induction of neural differentiation, hUCMs were seeded onto sterile glass slides and six various cocktails using a base medium (DMEM/LG) supplemented with 10 % FBS, retinoic acid (RA), dimethyl sulfoxide (DMSO), epidermal growth factor (EGF) and fibroblast growth factor (FGF) were used to compare their effect on neuronal, astrocyte and oligodandrocyte differentiation. The hUCMs were positive for mesenchymal markers, while they were negative for hematopoietic markers. Differentiation to adipogenic and osteogenic lineage was detected in these cells. Our data revealed that the cocktail consisting of DMEM/LG, FBS, RA, FGF, and EGF (DF/R/Fg/E group) induced hUCM cells to express the highest percentage of nestin, ß-tubulin III, neurofilament, and CNPase. The DF/Ds/Fg/E group led to the highest percentage of GFAP expression. While the expression levels of NF, GFAP, and CNPase were the lowest in the DF group. The least percentage of nestin and ß-tubulin III expression was observed in the DF/Ds group. We may conclude that FGF and EGF are important inducers for differentiation of hUCMs into neuron, astrocyte and oligodendrocyte. RA can induce hUCMs to differentiate into neuron and oligodendrocyte while for astrocyte differentiation DMSO had a pivotal role.

Deferoxamine Preconditioning of Neural-Like Cells Derived from Human Wharton’s Jelly Mesenchymal Stem Cells as a Strategy to Promote Their Tolerance and Therapeutic Potential: An In Vitro Study

“…For neural differentiation, according to the procedure described before (Salehinejad et al 2014), the HWJ-MSCs at the fourth passage were seeded into 75-cm 2 Costar culture flasks in a humidified atmosphere of 95 % air/5 % CO 2 at a density of 1 9 10 4 cells/ml with basic medium for 24 h. Then the cells were treated with 10 -2 M RA (retinol acetate; Sigma), 20 ng/ml EGF (epidermal growth factor; sigma), and 20 ng/ml bFGF (basic fibroblast growth factor; sigma) for 2 weeks.…”

Coadministration of the Human Umbilical Cord Matrix-Derived Mesenchymal Cells and Aspirin Alters Postischemic Brain Injury in Rats

“…7 Lu et al 8 has reported successful differentiation of bone marrow (BMS) cells into neural lineage including astrocytes and neurons. Also, Salehinejad et al 9 reported that hUCMs could successfully differentiate into various neural cells under certain conditions. Cell transplantation could have lead to the repair of damaged brain tissue and functional recovery in preclinical stroke models.…”

Effects of the two types of human cells on outgrowth of human glioma in rats

“…Immunocytochemistry procedure of the OECs was obtained from Salehinejad et al (36). Briefly, cultured cells were fixated, washed, incubated with primary antibodies, including mouse monoclonal anti-S 100 β (Chemicon) and mouse anti-p75 (Abcam, USA).…”