Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1

Abstract

Cell surface molecules have been implicated in cell interactions which underlie formation of the nervous system. The analysis of the functional properties of such molecules has profited from the combined use of antibodies and cell culture systems. It has been suggested that the interplay between these molecules modulates cell-to-cell interaction at critical developmental stages. In the mouse, N-CAM and L1 antigen have been shown to mediate Ca2+-independent adhesion among neural cells. N-CAM plays a role in fasciculation of neurites and formation of neuromuscular junction. L1 is apparently not involved in synaptogenesis, but in migration of granule cell neurones in the developing mouse cerebellar cortex. The two antigens are distinct molecular and functional entities which act synergistically in aggregation of neuroblastoma and early postnatal cerebellar cells. In view of a certain similarity in function between the two groups of molecules, it was not surprising to find that structural similarities are detectable by the monoclonal antibody L2. We show here that a carbohydrate moiety recognized by L2 and HNK-1 monoclonal antibodies, is present in mouse N-CAM and L1. The L2 epitope appears on all major neural cell types but not all N-CAM molecules express it. This heterogeneity points to a previously undetected molecular diversity which may have functional implications for modulating cell adhesion during development.

Specific Enzyme Complex of β-1,4-Galactosyltransferase-II and Glucuronyltransferase-P Facilitates Biosynthesis of N-linked Human Natural Killer-1 (HNK-1) Carbohydrate

“…1D, three major splicing isoforms of NCAMs (NCAM-120, NCAM-140, and NCAM-180) are detected, and no difference in expression levels of each NCAM isoform (Blot: NCAM) or in reactivity with Ricinus communis agglutinin (Blot: R. communis agglutinin was observed among three genotypes. In contrast, HNK-1 is predominantly expressed on NCAM-180 and NCAM-140 rather than on NCAM120 in wildtype mice brain, which is consistent with a previous report (30). The HNK-1 on NCAM disappeared in ␤4GalT-II-deficient mice (Blot: HNK-1).…”

Gene expression of CD24 core peptide molecule in developing brain and developing non‐neural tissues

“…Several adhesion molecules, such as N-cadherin, E-cadherin, P-cadherin, N-CAM, and Ng-CAM are suggested to be integrated in the interaction between migrating neuroblasts and radial glial cells, but no direct correlation between the localization of these adhesion molecules and the formation of the cortex structure in developing brain has been demonstrated (Hynes and Lander, 1992). Monoclonal antibody L2, which has been found to disrupt granule cell migration in vitro (Chuong et al, 1987), recognized the sugar structure of glycoproteins including neural cell adhesion molecule (N-CAM), L1 adhesion molecule, J1 glycoprotein, and the myelin-associated glycoprotein (MAG) (Kruse et al, 1984). The distribution of this epitope exhibits higher correlation to the brain regions of cortex formation while periaxonal localization of MAG suggests the possible role of this carbohydrate structure in the interactions between axons and myelinating cells (Sternberger et al, 1979).…”

CD57+/CD28− T cells in untreated hemato-oncological patients are expanded and display a Th1-type cytokine secretion profile, ex vivo cytolytic activity and enhanced tendency to apoptosis

“…3 The exact role of CD57 is unknown, although there is evidence for its involvement in cell-cell and cell-substrate interactions during ontogeny 1 and leukocyte trafficking via Pand L-selectin. 4 Within the lymphocyte compartment, CD57 is found on a subset of natural killer (NK) cells, as well as on subsets of CD4 + and CD8 + T lymphocytes.…”