Network structure of collagen layers absorbed on LB film

Chen, Qibin; Xu, Shouhong; Li, Rong; Liang, Xiaodong; Liu, Honglai

doi:10.1016/j.jcis.2007.07.059

Cited by 17 publications

(10 citation statements)

References 40 publications

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“…When the value of surface pressure is 30 mN/m, the worm-like structures appear, which are considered as the assembly of dots. A similar result was reported in our previous study on another Gemini surfactant with a rigid spacer [39]. The AFM images indicate that the 18-3-18,2Br −1 molecules have difficulty in forming the homogeneous film at the air/water interface.…”

Section: Afm Studies Of the Lb Monolayers Of 18-3-182br −1 And Sasupporting

confidence: 90%

Mixed monolayers of Gemini surfactants and stearic acid at the air/water interface

Chen

Zhang

et al. 2008

Journal of Colloid and Interface Science

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Section: Afm Studies Of the Lb Monolayers Of 18-3-182br −1 And Sasupporting

confidence: 90%

Mixed monolayers of Gemini surfactants and stearic acid at the air/water interface

Chen

Zhang

et al. 2008

Journal of Colloid and Interface Science

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“…In contrast to LS films, no fiber-like oriented networks were detected with Col I and Col IV dipcoated controls in IF stainings, implying that the fiber-like organization of the collagen networks is due to the application of the collagen solution on the air-subphase interface and subsequent compression [33]. The presence of the Col I and Col IV LS films in double layer LS structures was confirmed with SEM and IF analysis, in which the two oriented collagen layers could be distinguished by the different angle between the oriented fiber layers.…”

Section: Discussionmentioning

confidence: 71%

“…This reduction in expected thickness could be due to a lower binding affinity of the subsequent layers onto the preceding collagen layer governed by differences between the collagen-collagen interactions and those between collagen and the substrate [33,49]. Furthermore, loss of collagen during washing steps between the layer depositions as well as desorption of the salt crystals of the subphase during washing procedure could have also reduced the film thickness.…”

Section: Discussionmentioning

confidence: 99%

“…The Langmuir-Blodgett (LB) technique is an attractive approach for obtaining artificial biomimetic models with a well-characterized molecular organization without the use of additives and bio-incompatible materials [30,33,34]. This approach is based on the presence of collagen in its molecular form in acidic solution and its ability to form fibers resulting from the increase of the solvent pH when it is spread onto the subphase surface [35].…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

et al. 2015

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Skottman, H. (2015). Biomimetic collagen I and IV double layer Langmuir-Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells. BIOMATERIALS, 51, 257-269. DOI: 10.1016/j.biomaterials.2015 AbstractThe environmental cues received by the cells from synthetic substrates in vitro are very different from those they receive in vivo. In this study, we applied the Langmuir-Schaeffer (LS) deposition, a variant of Langmuir-Blodgett technique, to fabricate a biomimetic microenvironment mimicking the structure and organization of native Bruch's membrane for the production of the functional human embryonic stem cell derived retinal pigment epithelial (hESC-RPE) cells. Surface pressure-area isotherms were measured simultaneously with Brewster angle microscopy to investigate the self-assembly of human collagens type I and IV on airsubphase interface. Furthermore, the structure of the prepared collagen LS films was characterized with scanning electron microscopy, atomic force microscopy, surface plasmon resonance measurements and immunofluorescent staining. The integrity of hESC-RPE on double layer LS films was investigated by measuring transepithelial resistance and permeability of small molecular weight substance. Maturation and functionality of hESC-RPE cells on double layer collagen LS films was further assessed by RPE-specific gene and protein expression, growth factor secretion, and phagocytic activity. Here, we demonstrated that the prepared collagen LS films have layered structure with oriented fibers corresponding to architecture of the uppermost layers of Bruch's membrane and result in increased barrier properties and functionality of hESC-RPE cells as compared to the commonly used dip-coated controls.

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“…The Langmuir-Blodgett (LB) transfer has shown to be exceptional in the production of biomimetic models with well-controlled molecular organisation, such as collagen substrates [23,24]. The principle is that collagen at low pH occurs in its molecular form, but forms fibres when applied to the interface between air and phosphate buffered saline solution, since the charge neutralisation triggers the association between molecules.…”

Section: Introductionmentioning

confidence: 99%

Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering

et al. 2017

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Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye -the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch's membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability.Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE.Throughout the study, microporous films consistently showed better results as cell culture materials for 2 hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch's membranes for hESC-RPE transplantation.

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Network structure of collagen layers absorbed on LB film

Cited by 17 publications

References 40 publications

Mixed monolayers of Gemini surfactants and stearic acid at the air/water interface

Mixed monolayers of Gemini surfactants and stearic acid at the air/water interface

Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells

Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering

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