Network analysis to understand side effects of UVB on skin through transcriptomic approach

Kim, Su Ji; Na, Hye Won; Jang, Yu Jin; Shin, Dong Yeop; Choi, Hyun Jung; Kim, Hyoung June; Seo, Young Rok

doi:10.1007/s13273-021-00189-8

Cited by 3 publications

(5 citation statements)

References 28 publications

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“…To identify consistently regulated genes upon UVB exposure of skin, eight gene expression profile datasets were collected from the GEO and SRA databases considering the inclusion criteria. Specifically, six different RNA-Seq datasets were used, including one previously published dataset ( 28 ) and two microarrays. Furthermore, various skin cell types, such as skin biopsies, fibroblasts, and keratinocytes, were used to prevent biases toward single cell types.…”

Section: Resultsmentioning

confidence: 99%

“…Cell viability was measured to select an appropriate UVB intensity. NHEKs were irradiated at 13 mJ cm −2 based on the results of the cell viability test in our previous study ( 28 ). For NHDFs, the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was performed to confirm cell viability.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…RNA from NHEKs and NHDFs was extracted and the quality control of RNA samples was performed as described previously ( 28 ). cDNA was synthesized and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed as described previously ( 28 ). The primer sequences of four genes, GAPDH , IL1B , CCL2 , and LIF , and the thermal cycling conditions are shown in Supplementary Table S2 .…”

Section: Methodsmentioning

confidence: 99%

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Integrative analysis of RNA-sequencing and microarray for the identification of adverse effects of UVB exposure on human skin

Jang,

Na,

Shin

et al. 2024

Front. Public Health

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BackgroundUltraviolet B (UVB) from sunlight represents a major environmental factor that causes toxic effects resulting in structural and functional cutaneous abnormalities in most living organisms. Although numerous studies have indicated the biological mechanisms linking UVB exposure and cutaneous manifestations, they have typically originated from a single study performed under limited conditions.MethodsWe accessed all publicly accessible expression data of various skin cell types exposed to UVB, including skin biopsies, keratinocytes, and fibroblasts. We performed biological network analysis to identify the molecular mechanisms and identify genetic biomarkers.ResultsWe interpreted the inflammatory response and carcinogenesis as major UVB-induced signaling alternations and identified three candidate biomarkers (IL1B, CCL2, and LIF). Moreover, we confirmed that these three biomarkers contribute to the survival probability of patients with cutaneous melanoma, the most aggressive and lethal form of skin cancer.ConclusionOur findings will aid the understanding of UVB-induced cutaneous toxicity and the accompanying molecular mechanisms. In addition, the three candidate biomarkers that change molecular signals due to UVB exposure of skin might be related to the survival rate of patients with cutaneous melanoma.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Integrative analysis of RNA-sequencing and microarray for the identification of adverse effects of UVB exposure on human skin

Jang,

Na,

Shin

et al. 2024

Front. Public Health

Self Cite

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show abstract

“…UVB provides energy to the skin and is required to produce vitamin D. However, UVB can cause skin disorders such as inflammation, wrinkle formation, pigmentation irregularities, and loss of hydration. [2][3][4] UV irradiation disrupts collagen composition in the skin dermis by triggering excessive expression of matrix metalloproteinases (MMPs), which act as collagenases, thereby accelerating the process of skin aging. [5,6] Roses are edible flowers that are used as dietary supplements to treat catamenia disorder, trauma, blood disorders, diarrhea, and maintain hemostasis.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Photoaging by Anthocyanin Metabolites Derived from Rose Petal Extract

Sim,

Lee,

Lee

et al. 2024

Molecular Nutrition Food Res

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ScopeRose petal extract (RPE) shows a significant antioxidant effect through its anthocyanin content. However, the mechanism underlying the anti‐aging effects of orally administered RPE remains unclear. This study aims to describe the anti‐aging effect and mechanism of action of orally administered RPE in ultraviolet (UV)B‐induced skin aging.Methods and resultsThis study evaluates the protein expression of collagen type I alpha 1 (COL1A1) and matrix metalloproteinase 1 (MMP‐1) and the mRNA expression of hyaluronic synthase 2 (HAS2) in human dermal fibroblasts. In addition, the hyaluronidase and collagenase inhibitory activities of RPE are confirmed. To evaluate the anti‐aging effects of RPE, SKH‐1 hairless mice are administered RPE daily for 12 weeks. Wrinkle formation, transepidermal water loss (TEWL), and skin moisture loss induced by UVB irradiation are suppressed in the dorsal skin of SKH‐1 hairless mice orally administered RPE. Oral administration of RPE suppresses UVB irradiation‐induced collagen disruption and reduction of hyaluronic acid. To find the bioactive compound in the RPE, serum protocatechuic acid (PCA), an anthocyanin metabolite, is analyzed using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS).ConclusionAnthocyanins in RPE are metabolized to PCA in the body and circulated through the bloodstream to exhibit anti‐aging effects on the skin.

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Anti-melanogenic effects of porcine placenta peptides through inhibition of tyrosinase expression and activity

Lim,

Sim,

Baek

et al. 2024

Mol. Cell. Toxicol.

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Network analysis to understand side effects of UVB on skin through transcriptomic approach

Cited by 3 publications

References 28 publications

Integrative analysis of RNA-sequencing and microarray for the identification of adverse effects of UVB exposure on human skin

Integrative analysis of RNA-sequencing and microarray for the identification of adverse effects of UVB exposure on human skin

Inhibition of Photoaging by Anthocyanin Metabolites Derived from Rose Petal Extract

Anti-melanogenic effects of porcine placenta peptides through inhibition of tyrosinase expression and activity

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