Nerve Growth Factor (NGF)—Receptor Survival Axis in Head and Neck Squamous Cell Carcinoma

Dudás, József; Dietl, Wolfgang; Romani, Angela; Reinold, Susanne; Glueckert, Rudolf; Schrott‐Fischer, Anneliese; Dejaco, Daniel; Chacko, Lejo Johnson; Tuertscher, Raphaela; Schartinger, Volker Hans; Riechelmann, Herbert

doi:10.3390/ijms19061771

Cited by 25 publications

(34 citation statements)

References 53 publications

(81 reference statements)

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“…Pretreatment tumor samples were obtained during diagnostic panendoscopy. Patient samples were paraffin embedded, sectioned, and immuno-stained as published before [26].…”

Section: Patient Samples Immunohistochemistrymentioning

confidence: 99%

“…The immunofluorescent-stained slides were cell nuclei counterstained by DAPI (Molecular Probes) and covered in Vectashield Vibrance (Vector Laboratories, Burlingame, CA, USA). Immunohistochemical reactions were acquired in TissueFaxs system and quantified using TissueQuest software as published before [26].…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Routinely cultured cell lines and mixed cultures were collected by centrifugation and embedded as cell pellets in agarose as published before [26,30] and modified as follows: Cells were harvested by centrifugation at 290 × g for 10 min at 4°C, and the resulting pellet was fixed in 10 mL neutral buffered 4% formaldehyde solution (SAV Liquid Production Ltd., Flintsbach am Inn, Germany). After fixation the cells were centrifuged at 400 × g for 10 min at room temperature.…”

Section: Paraffin Embedding Of Cultured Cellsmentioning

confidence: 99%

“…After that, the tube was placed on ice, the cell pellet was trimmed, and it was placed in embedding cassette. The cell pellet in the cassette was stored in PBS containing 0.05-0.1% sodium azide until embedded in paraffin as published in detail before [26].…”

Section: Paraffin Embedding Of Cultured Cellsmentioning

confidence: 99%

“…The cell sections were stained immunohistochemically identical to the tissue sections. The percentage of positive cells for the required reaction was identified after scanning the sections in the TissueFaxs system and evaluating with Tissuequest software [26].…”

Section: Paraffin Embedding Of Cultured Cellsmentioning

confidence: 99%

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Pleiotropic Effects of Epithelial Mesenchymal Crosstalk on Head and Neck Cancer: EMT and beyond

Steinbichler

Savic

Dejaco

et al. 2019

Cancer Microenvironment

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Epithelial mesenchymal crosstalk (EMC) describes the interaction of the tumor stroma and associated fibroblasts with epithelial cancer cells. In this study we analysed the effects of EMC on head and neck cancer cells. In tumor cell lines EMC was induced using media conditioned from a mix-culture of cancer cells and fibroblasts. Cell proliferation and chemotherapy response were assessed using direct cell counting. Flow cytometry, immunohistochemistry of markers of epithelial-mesenchymal transition (EMT) and subsequent TissueFaxs™ acquisition and quantification and western blot analysis were performed. Holotomographic microscopy imaging was used to visualize the effects of EMC on Cisplatin response of SCC-25 cells. EMC induced a hybrid epithelial-mesenchymal phenotype in SCC-25 cells with co-expression of vimentin and cytokeratin. This hybrid phenotype was associated with chemotherapy resistance and increased proliferation of the cells. The EMC conditioned medium led to an activation of the IL-6/STAT3 pathway with subsequent phosphorylation of STAT3. EMC induced a hybrid epithelialmesenchymal phenotype in HNSCC cells accompanied by increased therapy resistance and cell proliferation. The IL-6/ STAT3 pathway might be one of the major pathways involved in these EMC-related effects.

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“…Pretreatment tumor samples were obtained during diagnostic panendoscopy. Patient samples were paraffin embedded, sectioned, and immuno-stained as published before [26].…”

Section: Patient Samples Immunohistochemistrymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Paraffin Embedding Of Cultured Cellsmentioning

confidence: 99%

Section: Paraffin Embedding Of Cultured Cellsmentioning

confidence: 99%

Section: Paraffin Embedding Of Cultured Cellsmentioning

confidence: 99%

See 3 more Smart Citations

Pleiotropic Effects of Epithelial Mesenchymal Crosstalk on Head and Neck Cancer: EMT and beyond

Steinbichler

Savic

Dejaco

et al. 2019

Cancer Microenvironment

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Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study

Giotakis

Dudás

Glueckert

et al. 2020

Histochem Cell Biol

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Epithelial, connective tissue and immune cells contribute in various ways to the pathophysiology of chronic rhinosinusitis (CRS). However, data of their distribution in upper airway mucosa are sparse. We aimed to provide quantitative, purely informative data on the distribution of these cell lineages and their coexpression patterns, which might help identifying, e.g., cells in the epithelium undergoing through epithelial–mesenchymal transition (EMT). For this purpose, we used immunofluorescence multichannel image cytometry (IMIC). We examined fixed paraffin-embedded tissue samples (FFPE) of six patients with chronic rhinosinusitis (CRS) and of three patients without CRS (controls). The direct-conjugated antibodies pancytokeratin, vimentin and CD45/CD18 were used for coexpression analysis in epithelial layer and lamina propria. Image acquisition and analysis were performed with TissueFAXS and StrataQuest, respectively. To distinguish positive from negative expression, a ratio between cell-specific immunostaining intensity and background was developed. Isotype controls were used as negative controls. Per patient, a 4.5-mm2 tissue area was scanned and a median of 14,875 cells was recognized. The most common cell types were cytokeratin-single-positive (26%), vimentin-single-positive (13%) and CD45/CD18-single-positive with CD45/CD18–vimentin-double-positive cells (29%). In the patients with CRS, CD45/CD18-single-positive cells were 3–6 times higher compared to the control patients. In the epithelial layer, cytokeratin–vimentin-double-positive EMT cells were observed 3–5 times higher in the patients with CRS than in the control patients. This study provided quantitative data for the distribution of crucial cell types in CRS. Future studies may focus on the distribution and coexpression patterns of different immune cells in CRS or even cancer tissue.

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Early appearance of key transcription factors influence the spatiotemporal development of the human inner ear

et al. 2019

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Expression patterns of transcription factors leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), transforming growth factor-β-activated kinase-1 (TAK1), SRY (sex-determining region Y)-box 2 (SOX2), and GATA binding protein 3 (GATA3) in the developing human fetal inner ear were studied between the gestation weeks 9 and 12. Further development of cochlear apex between gestational weeks 11 and 16 (GW11 and GW16) was examined using transmission electron microscopy.LGR5 was evident in the apical poles of the sensory epithelium of the cochlear duct and the vestibular end organs at GW11. Immunostaining was limited to hair cells of the organ of Corti by GW12. TAK1 was immune positive in inner hair cells of the organ of Corti by GW12 and colocalized with p75 neurotrophic receptor expression. Expression for SOX2 was confined primarily to the supporting cells of utricle at the earliest stage examined at GW9. Intense expression for GATA3 was presented in the cochlear sensory epithelium and spiral ganglia at GW9. Expression of GATA3 was present along the midline of both the utricle and saccule in the zone corresponding to the striolar reversal zone where the hair cell phenotype switches from type I to type II. The spatiotemporal gradient of the development of the organ of Corti was also evident with the apex of the cochlea forming by GW16. It seems that highly specific staining patterns of several transcriptions factors are critical in guiding the genesis of the inner ear over development. Our findings suggest that the spatiotemporal gradient in cochlear development extends at least until gestational week 16.

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Nerve Growth Factor (NGF)—Receptor Survival Axis in Head and Neck Squamous Cell Carcinoma

Cited by 25 publications

References 53 publications

Pleiotropic Effects of Epithelial Mesenchymal Crosstalk on Head and Neck Cancer: EMT and beyond

Pleiotropic Effects of Epithelial Mesenchymal Crosstalk on Head and Neck Cancer: EMT and beyond

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study

Early appearance of key transcription factors influence the spatiotemporal development of the human inner ear

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