Nephelometric Assay of Immunoglobulin G Chemically Bound to Chloromethyl Styrene Beads

Abstract

Monodisperse core–shell stable latexes with reactive methylchloride surface functionalities were prepared at two different reaction temperatures. The reaction temperature played an important role in the amount of reactive functional groups. The covalent coupling had an efficiency of more than 50%. Antibodies covalently bound to functionalized polystyrene beads were used to detect corresponding antigens by nephelometry.

Computational Design of a Carbon Nanotube Fluorofullerene Biosensor

“…The surface of the soluble polymer beads will be functionalized with antibodies or complementary Fab fragments to that on the fluorofullerene with synthesis according to the procedures described by Uchida et al [56], van Erp et al [57], Sarobe et al [58], and Kawaguchi [55]. For example, Sarobe et al [58] covalently coupled immunoglobulin G (IgG) to chloromethylstyrene beads to create stable sensitized latex beads. Uchida et al [56] immobilized both intact IgG or Fab fragments of antibovine serum albumin antibody to polymer microspheres using an oxidized dextran as a binder.…”

Computational Design of a Carbon Nanotube Fluorofullerene Biosensor

“…The surface of the soluble polymer beads will be functionalized with antibodies or complementary Fab fragments to that on the fluorofullerene with synthesis according to the procedures described by Uchida et al [56], van Erp et al [57], Sarobe et al [58], and Kawaguchi [55]. For example, Sarobe et al [58] covalently coupled immunoglobulin G (IgG) to chloromethylstyrene beads to create stable sensitized latex beads. Uchida et al [56] immobilized both intact IgG or Fab fragments of antibovine serum albumin antibody to polymer microspheres using an oxidized dextran as a binder.…”

Identification and Pharmacognostic Control of the Species: Mentha X Villosa, Lippia Alba, Cymbopogon Citratus and Melissa Officinalis

“…Covalent binding was investigated by synthesisizing of surface-modified latex particles. The incorporation of monomers containing functional groups such as --CH 2 Cl or -NH 2 has been targeted for covalent binding of -NH 2 or -COOH groups [41]; [43,[45][46]), from biomolecules. Modification of amino groups with poly (maleic anhydride-alt-methyl vinyl ether) (MAMVE) has been performed to study the oriented binding of peptides modified with a cationic tail [49].…”

Studies about polymers, their interactions with the immune system and applications in immunotechnology in Uruguay: a systematic review / Estudos sobre polímeros, suas interações com o sistema imunológico e aplicações em imunotecnologia no Uruguai: uma revisão sistemática

“…or -NH2 has been targeted for covalent binding of -NH2 or -COOH groups [41];43,45-46), from biomolecules. Modification of amino groups with poly(maleic anhydride-altmethyl vinyl ether) (MAMVE) has been performed to study the oriented binding of peptides modified with a cationic tail [49].…”

Computational Design of a Carbon Nanotube Fluorofullerene Biosensor

“…The surface of the soluble polymer beads will be functionalized with antibodies or complementary Fab fragments to that on the fluorofullerene with synthesis according to the procedures described by Uchida et al [56], van Erp et al [57], Sarobe et al [58], and Kawaguchi [55]. For example, Sarobe et al [58] covalently coupled immunoglobulin G (IgG) to chloromethylstyrene beads to create stable sensitized latex beads. Uchida et al [56] immobilized both intact IgG or Fab fragments of antibovine serum albumin antibody to polymer microspheres using an oxidized dextran as a binder.…”

Identification and Pharmacognostic Control of the Species: Mentha X Villosa, Lippia Alba, Cymbopogon Citratus and Melissa Officinalis

“…Covalent binding was investigated by synthesisizing of surface-modified latex particles. The incorporation of monomers containing functional groups such as --CH 2 Cl or -NH 2 has been targeted for covalent binding of -NH 2 or -COOH groups [41]; [43,[45][46]), from biomolecules. Modification of amino groups with poly (maleic anhydride-alt-methyl vinyl ether) (MAMVE) has been performed to study the oriented binding of peptides modified with a cationic tail [49].…”

Studies about polymers, their interactions with the immune system and applications in immunotechnology in Uruguay: a systematic review / Estudos sobre polímeros, suas interações com o sistema imunológico e aplicações em imunotecnologia no Uruguai: uma revisão sistemática

“…or -NH2 has been targeted for covalent binding of -NH2 or -COOH groups [41];43,45-46), from biomolecules. Modification of amino groups with poly(maleic anhydride-altmethyl vinyl ether) (MAMVE) has been performed to study the oriented binding of peptides modified with a cationic tail [49].…”

Computational Design of a Carbon Nanotube Fluorofullerene Biosensor

“…The surface of the soluble polymer beads will be functionalized with antibodies or complementary Fab fragments to that on the fluorofullerene with synthesis according to the procedures described by Uchida et al [56], van Erp et al [57], Sarobe et al [58], and Kawaguchi [55]. For example, Sarobe et al [58] covalently coupled immunoglobulin G (IgG) to chloromethylstyrene beads to create stable sensitized latex beads. Uchida et al [56] immobilized both intact IgG or Fab fragments of antibovine serum albumin antibody to polymer microspheres using an oxidized dextran as a binder.…”

Identification and Pharmacognostic Control of the Species: Mentha X Villosa, Lippia Alba, Cymbopogon Citratus and Melissa Officinalis

“…Covalent binding was investigated by synthesisizing of surface-modified latex particles. The incorporation of monomers containing functional groups such as --CH 2 Cl or -NH 2 has been targeted for covalent binding of -NH 2 or -COOH groups [41]; [43,[45][46]), from biomolecules. Modification of amino groups with poly (maleic anhydride-alt-methyl vinyl ether) (MAMVE) has been performed to study the oriented binding of peptides modified with a cationic tail [49].…”

Studies about polymers, their interactions with the immune system and applications in immunotechnology in Uruguay: a systematic review / Estudos sobre polímeros, suas interações com o sistema imunológico e aplicações em imunotecnologia no Uruguai: uma revisão sistemática

“…or -NH2 has been targeted for covalent binding of -NH2 or -COOH groups [41];43,45-46), from biomolecules. Modification of amino groups with poly(maleic anhydride-altmethyl vinyl ether) (MAMVE) has been performed to study the oriented binding of peptides modified with a cationic tail [49].…”