Neosporosis in Animals

Dubey, J. P.; Hemphill, Andrew; Calero‐Bernal, Rafael; Schares, Gereon

doi:10.1201/9781315152561

Cited by 125 publications

(167 citation statements)

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“…The in vitro cultivation of N. caninum in 1988 in USA made it possible to develop diagnostic tests for neosporosis (DUBEY et al, 2017). However, unlike T. gondii, it is difficult to culture N. caninum (see later discussion) and only one isolate (NC-1) was available initially at the USDA laboratory in Beltsville, Maryland, USA.…”

Section: History Of Neosporosis In Brazilmentioning

confidence: 99%

“…For example, in the indirect fluorescent antibody test (IFAT) and the Neospora agglutination test (NAT) whole tachyzoites are used detecting antibodies to surface proteins whereas in the enzyme-linked immunoabsorbent assay (ELISA) different antigens are used, some of them using crude tachyzoite extract while others used soluble antigens (Table 2a). The standardization of serological tests was based on studies in other countries and a full discussion of these is beyond the scope of this review-this subject was recently reviewed (DUBEY et al, 2017). One of the problems with serological testing is the availability of standardized sera from experimentally infected animals.…”

Section: Epidemiologymentioning

confidence: 99%

“…In addition, to provide these Neospora isolates for biological and genetic studies to researchers in other countries (DUBEY et al, 2017), García-Melo in association with researchers from Spain did the first microsatellite typing data for Nc-Goiás isolated from clinically healthy cattle. Although the isolate had most of the alleles already identified, unique alleles were described for this strain at the MS5 and MS10 loci, using 12 microsatellite markers (GARCÍA-MELO et al, 2009).…”

Section: Viable N Caninum Isolates and Genetic Diversitymentioning

confidence: 99%

“…Considerable progress has been made in understanding the biology of N. caninum. A recent book on neosporosis (DUBEY et al, 2017) listed more than 2100 citations and most reports (>200) from Brazil. Many of the reports from Brazil are scattered in local journals.…”

Section: Introductionmentioning

confidence: 99%

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All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

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Neospora caninum is protozoan parasite with domestic and wild dogs, coyotes and grey wolves as the definitive hosts and many warm-blooded animals as intermediate hosts. It was cultivated and named in 1988. Neosporosis is a major disease of cattle and has no public health significance. Since 1990's N. caninum has emerged as a major cause of abortion in cattle worldwide, including in Brazil. N. caninum also causes clinical infections in several other animal species. Considerable progress has been made in understanding the biology of N. caninum and there are more than 200 papers on this subject from Brazil. However, most of the reports on neosporosis from Brazil are serological surveys. Overall, little is known of clinical neosporosis in Brazil, particularly cattle. The few reports pertain to sporadic cases of abortion with no information on epidemics or storms of abortion. The objective of the present review is to summarize all reports from Brazil and suggest topic for further research, including prevalence of N. caninum oocysts in soil or in canine feces, and determining if there are additional definitive hosts, other than the domestic dog. There is need for a national survey in cattle using defined parameters. Future researches should focus on molecular characterization of N. caninum strains, possibility of vaccine production and relationship between wildlife and livestock epidemiology.Keywords: Neospora caninum, neosporosis, domestic animals, wild animals, Brazil. ResumoNeospora caninum é um protozoário parasita que possui os canídeos domésticos e selvagens, coiotes e lobos cinzentos como hospedeiros definitivos e vários animais de sangue quente como hospedeiros intermediários. Foi cultivado e nomeado em 1988. A neosporose é uma das principais doenças em bovinos e não tem significância em saúde pública. Desde 1990, N. caninum tem emergido como uma das principais causas de aborto em bovinos em todo o mundo, inclusive no Brasil. N. caninum também causa infecções clínicas em várias outras espécies animais. Consideráveis avanços foram feitos na compreensão da biologia desse parasita e há mais de 200 trabalhos sobre o assunto no Brasil. No entanto, a maioria dos relatos de neosporose do Brasil são relacionados a sorologia. Em geral, pouco se sabe sobre a neosporose clínica no Brasil, particularmente em bovinos. Os poucos relatos referem-se a casos esporádicos de aborto sem informações sobre epidemias ou surtos de aborto. O objetivo da presente revisão é resumir todos os relatos sobre N. caninum no Brasil e sugerir tópicos para pesquisas futuras, incluindo a prevalência de oocistos de N. caninum no solo ou em fezes caninas e determinar se há hospedeiros definitivos adicionais, exceto o cão doméstico no país. Uma pesquisa nacional em bovinos usando parâmetros definidos seria de grande importância. Pesquisas futuras deveriam ser focadas na caracterização de cepas de N. caninum, possibilidade de produção de vacinas e a relação epidemiológica entre a vida selvagem e o gado.Palavras-chave: Neospora caninum, neospo...

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Section: History Of Neosporosis In Brazilmentioning

confidence: 99%

Section: Epidemiologymentioning

confidence: 99%

Section: Viable N Caninum Isolates and Genetic Diversitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…The protozoa Toxoplasma gondii, Neospora spp., and Sarcocystis neurona belong to the phylum Apicomplexa and have been correlated with animal diseases (DUBEY, 2010;DUBEY et al, 2016;DUBEY et al, 2017), furthermore T. gondii is also globally correlated with human disease (DUBEY, 2010).…”

mentioning

confidence: 99%

Occurrences of antibodies against Toxoplasma gondii, Neospora spp., and Sarcocystis neurona in horses and dogs in the municipality of Pauliceia, São Paulo, Brazil

Oliveira

Silva

Silveira

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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Serum samples from 116 horses and 47 dogs from the municipality of Paulicéia, in the state of São Paulo, Brazil, were examined for anti-Toxoplasma gondii, -Neospora spp. and -Sarcocystis neurona antibodies by means of the indirect fluorescent antibody test (IFAT). The results showed that only one horse was seropositive for T. gondii (0.9%) and anti-Neospora spp. antibodies were detected in three out of the 116 horses tested (2.6%). However, 27 horses showed antibodies against S. neurona (23.8%). Amongst the serum samples from the dogs, 10 out of the 47 dogs showed antibodies against T. gondii (21.3%) and three dogs showed antibodies against Neospora caninum (6.4%). This study reports that in the municipality of Paulicéia dogs in both the rural and the urban area were exposed to T. gondii and N. caninum, while horses in the rural area were exposed to all three protozoa studied, with high occurrence of anti-S. neurona antibodies. Keywords: Toxoplasmosis. Neosporosis. Equine protozoal myeloencephalitis. IFAT. ResumoAmostras de soro de 116 equinos e 47 cães provenientes do município de Paulicéia, São Paulo, foram testadas para detecção de anticorpos anti-Toxoplasma gondii, -Neospora spp. e -Sarcocystis neurona por meio da reação de imunofluorescência indireta (RIFI). Apenas um equino, entre 116 equinos testados, teve diagnóstico soropositivo para T. gondii (0,9%), e três deles (2,6%) apresentaram anticorpos anti-Neospora spp. Entretanto, 27 equinos apresentaram anticorpos anti-S. neurona (23,8%). Nas amostras de cães, dez dos 47 animais apresentaram anticorpos anti-T. gondii (21,3%) e três tiveram diagnóstico soropositivo para Neospora caninum (6,4%). Este estudo relata que no município de Paulicéia os cães das áreas urbana e rural foram expostos a T. gondii e N. caninum, enquanto os equinos da área rural foram expostos aos três protozoários estudados, com alta ocorrência de anticorpos anti-S. neurona. Palavras-chave: Toxoplasmose. Neosporose. Mieloencefalite protozoária equina. RIFI.

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First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia

Amdouni

Abedennebi²,

Amairia

et al. 2022

Veterinary Medicine & Sci

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Background Neospora caninum has been documented to infect most domestic wildlife but is known to primarily infect dogs and cattle and is considered an important cause of abortion in camels. Objective The aim of this study was to estimate the molecular detection of Neospora caninum in tissues of naturally infected camelids. Methods Brain, tongue (bottom and tip) and masseter muscles from 35 slaughtered camelids from Tataouine and Médenine regions were collected (n = 140 samples). PCR was used to amplify and detect N. caninum DNA in tissues samples followed by sequencing of some PCR products. A phylogenetic tree was then constructed to compare the partial sequences of the ITS1 gene with GenBank sequences. Histopathology examination was used to detect Neospora spp. cysts, but no lesions were observed. Results The overall molecular detection of N. caninum in camelids was 34.3% (12/35). The highest molecular detection of N. caninum was recorded in animals of more than 3 years old (6/9) and in animals aged between 1 and 3 years old (4/12). Whilst, the lowest molecular detection (2/14) was observed in animals 1 year or younger (p = 0.035). There were no significant differences in molecular detection of N. caninum according to both locality and gender (p > 0.05). Similarly, there was no difference of prevalence between different anatomical locations. Comparison of the partial sequences of the ITS1 gene revealed 100–95.5% similarity among our N. caninum amplicon (MW551566) and those deposited in GenBank. Conclusion These results highlight the presence of a risk infection by N. caninum in camels. For preventing N. caninum infection further studies are needed to improve our knowledge about the epidemiology of neosporosis in North Africa.

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Neosporosis in Animals

Cited by 125 publications

References 0 publications

All about neosporosis in Brazil

All about neosporosis in Brazil

Occurrences of antibodies against Toxoplasma gondii, Neospora spp., and Sarcocystis neurona in horses and dogs in the municipality of Pauliceia, São Paulo, Brazil

First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia

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